Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3539 and EX28DRAFT_3540 are separated by 182 nucleotides EX28DRAFT_3540 and EX28DRAFT_3541 overlap by 29 nucleotides
EX28DRAFT_3539: EX28DRAFT_3539 - 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component, at 202,589 to 204,151
_3539
EX28DRAFT_3540: EX28DRAFT_3540 - 4-hydroxyphenylacetate catabolism regulatory protein HpaA, at 204,334 to 205,266
_3540
EX28DRAFT_3541: EX28DRAFT_3541 - 4-hydroxyphenylacetate permease, at 205,238 to 206,590
_3541
Position (kb) 204
205
206 Strain fitness (log2 ratio) -2
-1
0
1
2 at 203.544 kb on + strand, within EX28DRAFT_3539 at 203.628 kb on + strand, within EX28DRAFT_3539 at 203.732 kb on - strand, within EX28DRAFT_3539 at 203.757 kb on + strand, within EX28DRAFT_3539 at 203.985 kb on + strand, within EX28DRAFT_3539 at 204.057 kb on + strand at 204.057 kb on + strand at 204.061 kb on + strand at 204.061 kb on + strand at 204.063 kb on + strand at 204.063 kb on + strand at 204.063 kb on + strand at 204.063 kb on + strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.092 kb on + strand at 204.151 kb on + strand at 204.793 kb on - strand, within EX28DRAFT_3540 at 204.933 kb on - strand, within EX28DRAFT_3540 at 205.237 kb on - strand at 205.363 kb on + strand at 205.363 kb on + strand at 205.364 kb on - strand at 205.503 kb on - strand, within EX28DRAFT_3541 at 205.809 kb on + strand, within EX28DRAFT_3541 at 206.129 kb on - strand, within EX28DRAFT_3541
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 203,544 + EX28DRAFT_3539 0.61 +0.2 203,628 + EX28DRAFT_3539 0.66 -0.2 203,732 - EX28DRAFT_3539 0.73 -1.0 203,757 + EX28DRAFT_3539 0.75 -0.5 203,985 + EX28DRAFT_3539 0.89 +0.1 204,057 + -0.4 204,057 + -0.9 204,061 + -0.1 204,061 + -0.1 204,063 + -1.1 204,063 + -2.4 204,063 + +1.2 204,063 + +0.4 204,064 - -0.4 204,064 - -0.2 204,064 - +0.6 204,064 - -0.7 204,064 - -0.6 204,092 + +0.3 204,151 + -0.3 204,793 - EX28DRAFT_3540 0.49 -0.9 204,933 - EX28DRAFT_3540 0.64 -1.0 205,237 - +0.4 205,363 + -0.2 205,363 + +1.2 205,364 - -0.2 205,503 - EX28DRAFT_3541 0.20 +1.8 205,809 + EX28DRAFT_3541 0.42 -0.2 206,129 - EX28DRAFT_3541 0.66 -0.2
Or see this region's nucleotide sequence