Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3420 and EX28DRAFT_3421 are separated by 95 nucleotides EX28DRAFT_3421 and EX28DRAFT_3422 are separated by 95 nucleotides EX28DRAFT_3422 and EX28DRAFT_3423 are separated by 41 nucleotides EX28DRAFT_3423 and EX28DRAFT_3424 are separated by 332 nucleotides
EX28DRAFT_3420: EX28DRAFT_3420 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase, at 91,550 to 92,929
_3420
EX28DRAFT_3421: EX28DRAFT_3421 - Uncharacterized protein conserved in bacteria, at 93,025 to 93,576
_3421
EX28DRAFT_3422: EX28DRAFT_3422 - Predicted Zn-dependent proteases and their inactivated homologs, at 93,672 to 95,024
_3422
EX28DRAFT_3423: EX28DRAFT_3423 - Soluble cytochrome b562, at 95,066 to 95,452
_3423
EX28DRAFT_3424: EX28DRAFT_3424 - PRD domain protein EF_0829/AHA_3910, at 95,785 to 96,105
_3424
Position (kb)
93
94
95
96 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 92.722 kb on + strand, within EX28DRAFT_3420 at 93.571 kb on - strand at 93.655 kb on + strand at 93.655 kb on + strand at 93.671 kb on + strand at 93.691 kb on + strand at 93.844 kb on + strand, within EX28DRAFT_3422 at 93.845 kb on - strand, within EX28DRAFT_3422 at 93.882 kb on + strand, within EX28DRAFT_3422 at 93.883 kb on - strand, within EX28DRAFT_3422 at 93.977 kb on + strand, within EX28DRAFT_3422 at 93.978 kb on - strand, within EX28DRAFT_3422 at 94.170 kb on + strand, within EX28DRAFT_3422 at 94.284 kb on + strand, within EX28DRAFT_3422 at 94.665 kb on + strand, within EX28DRAFT_3422 at 94.934 kb on + strand at 94.968 kb on - strand at 94.973 kb on + strand at 94.974 kb on - strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.975 kb on + strand at 94.976 kb on - strand at 94.976 kb on - strand at 94.976 kb on - strand at 94.976 kb on - strand at 94.976 kb on - strand at 94.976 kb on - strand at 94.991 kb on - strand at 95.025 kb on + strand at 95.042 kb on + strand at 95.042 kb on + strand at 95.313 kb on - strand, within EX28DRAFT_3423 at 95.368 kb on + strand, within EX28DRAFT_3423 at 95.516 kb on - strand at 95.519 kb on + strand at 95.520 kb on - strand at 95.597 kb on + strand at 95.629 kb on - strand at 95.648 kb on + strand at 95.649 kb on - strand at 95.649 kb on - strand at 95.708 kb on + strand at 95.835 kb on + strand, within EX28DRAFT_3424 at 95.835 kb on + strand, within EX28DRAFT_3424 at 95.835 kb on + strand, within EX28DRAFT_3424 at 95.851 kb on + strand, within EX28DRAFT_3424 at 95.851 kb on + strand, within EX28DRAFT_3424 at 95.891 kb on + strand, within EX28DRAFT_3424
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 92,722 + EX28DRAFT_3420 0.85 -0.8 93,571 - -0.8 93,655 + +0.7 93,655 + -0.1 93,671 + -0.4 93,691 + +0.9 93,844 + EX28DRAFT_3422 0.13 +0.7 93,845 - EX28DRAFT_3422 0.13 +0.6 93,882 + EX28DRAFT_3422 0.16 +0.7 93,883 - EX28DRAFT_3422 0.16 -1.2 93,977 + EX28DRAFT_3422 0.23 -0.3 93,978 - EX28DRAFT_3422 0.23 -0.9 94,170 + EX28DRAFT_3422 0.37 -0.2 94,284 + EX28DRAFT_3422 0.45 +2.4 94,665 + EX28DRAFT_3422 0.73 +0.4 94,934 + +0.3 94,968 - -0.4 94,973 + +0.0 94,974 - -0.2 94,975 + -0.6 94,975 + -0.2 94,975 + -0.3 94,975 + -0.2 94,975 + -0.2 94,975 + -0.4 94,975 + +0.0 94,975 + +0.9 94,975 + -0.8 94,975 + -0.2 94,976 - -0.1 94,976 - -0.5 94,976 - +0.3 94,976 - -0.3 94,976 - -0.7 94,976 - +0.1 94,991 - +0.8 95,025 + +0.0 95,042 + -0.2 95,042 + +0.1 95,313 - EX28DRAFT_3423 0.64 -0.4 95,368 + EX28DRAFT_3423 0.78 +0.8 95,516 - -0.5 95,519 + +0.3 95,520 - -0.3 95,597 + -0.3 95,629 - -1.1 95,648 + -0.5 95,649 - -0.1 95,649 - -1.9 95,708 + +0.0 95,835 + EX28DRAFT_3424 0.16 -0.6 95,835 + EX28DRAFT_3424 0.16 +0.9 95,835 + EX28DRAFT_3424 0.16 +0.2 95,851 + EX28DRAFT_3424 0.21 +0.1 95,851 + EX28DRAFT_3424 0.21 -0.5 95,891 + EX28DRAFT_3424 0.33 -0.3
Or see this region's nucleotide sequence