Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3400 and EX28DRAFT_3401 are separated by 190 nucleotides EX28DRAFT_3401 and EX28DRAFT_3402 overlap by 7 nucleotides EX28DRAFT_3402 and EX28DRAFT_3403 are separated by 324 nucleotides
EX28DRAFT_3400: EX28DRAFT_3400 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 68,823 to 70,766
_3400
EX28DRAFT_3401: EX28DRAFT_3401 - 3'(2'),5'-bisphosphate nucleotidase, bacterial, at 70,957 to 71,697
_3401
EX28DRAFT_3402: EX28DRAFT_3402 - conserved hypothetical protein YtfJ-family, TIGR01626, at 71,691 to 72,248
_3402
EX28DRAFT_3403: EX28DRAFT_3403 - Protein of unknown function (DUF1107), at 72,573 to 72,779
_3403
Position (kb) 70
71
72 Strain fitness (log2 ratio) -2
-1
0
1
2 at 70.039 kb on + strand, within EX28DRAFT_3400 at 70.040 kb on - strand, within EX28DRAFT_3400 at 70.144 kb on + strand, within EX28DRAFT_3400 at 70.145 kb on - strand, within EX28DRAFT_3400 at 70.394 kb on - strand, within EX28DRAFT_3400 at 70.394 kb on - strand, within EX28DRAFT_3400 at 70.439 kb on + strand, within EX28DRAFT_3400 at 70.440 kb on - strand, within EX28DRAFT_3400 at 70.465 kb on + strand, within EX28DRAFT_3400 at 70.466 kb on - strand, within EX28DRAFT_3400 at 70.510 kb on + strand, within EX28DRAFT_3400 at 70.510 kb on + strand, within EX28DRAFT_3400 at 70.608 kb on + strand at 70.643 kb on - strand at 70.646 kb on - strand at 70.692 kb on - strand at 70.701 kb on + strand at 70.754 kb on + strand at 70.804 kb on + strand at 70.804 kb on + strand at 70.808 kb on + strand at 70.809 kb on - strand at 70.903 kb on - strand at 70.908 kb on - strand at 71.452 kb on + strand, within EX28DRAFT_3401 at 71.452 kb on + strand, within EX28DRAFT_3401 at 71.644 kb on + strand at 71.646 kb on + strand at 71.646 kb on + strand at 71.692 kb on + strand at 71.693 kb on - strand at 72.050 kb on + strand, within EX28DRAFT_3402 at 72.051 kb on - strand, within EX28DRAFT_3402 at 72.051 kb on - strand, within EX28DRAFT_3402 at 72.089 kb on + strand, within EX28DRAFT_3402 at 72.103 kb on + strand, within EX28DRAFT_3402 at 72.104 kb on - strand, within EX28DRAFT_3402 at 72.104 kb on - strand, within EX28DRAFT_3402 at 72.108 kb on - strand, within EX28DRAFT_3402 at 72.114 kb on - strand, within EX28DRAFT_3402 at 72.233 kb on + strand at 72.233 kb on + strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.240 kb on + strand at 72.299 kb on + strand at 72.299 kb on + strand at 72.300 kb on - strand at 72.300 kb on - strand at 72.300 kb on - strand at 72.305 kb on + strand at 72.305 kb on + strand at 72.306 kb on - strand at 72.364 kb on + strand at 72.364 kb on + strand at 72.365 kb on - strand at 72.388 kb on + strand at 72.454 kb on - strand at 72.474 kb on + strand at 72.485 kb on + strand at 72.485 kb on + strand at 72.486 kb on - strand at 72.486 kb on - strand at 72.488 kb on - strand at 72.551 kb on - strand at 72.567 kb on - strand at 72.591 kb on + strand at 72.592 kb on - strand at 72.592 kb on - strand at 72.615 kb on + strand, within EX28DRAFT_3403 at 72.616 kb on - strand, within EX28DRAFT_3403 at 72.645 kb on + strand, within EX28DRAFT_3403 at 72.645 kb on + strand, within EX28DRAFT_3403 at 72.645 kb on + strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403 at 72.646 kb on - strand, within EX28DRAFT_3403
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 70,039 + EX28DRAFT_3400 0.63 -0.2 70,040 - EX28DRAFT_3400 0.63 +0.2 70,144 + EX28DRAFT_3400 0.68 -0.1 70,145 - EX28DRAFT_3400 0.68 -0.1 70,394 - EX28DRAFT_3400 0.81 -0.1 70,394 - EX28DRAFT_3400 0.81 -0.5 70,439 + EX28DRAFT_3400 0.83 +1.0 70,440 - EX28DRAFT_3400 0.83 -0.2 70,465 + EX28DRAFT_3400 0.84 -0.2 70,466 - EX28DRAFT_3400 0.85 -0.2 70,510 + EX28DRAFT_3400 0.87 +1.8 70,510 + EX28DRAFT_3400 0.87 -0.5 70,608 + -0.3 70,643 - -0.6 70,646 - -0.5 70,692 - +0.2 70,701 + +0.3 70,754 + +1.0 70,804 + -0.6 70,804 + -0.2 70,808 + +1.1 70,809 - -0.4 70,903 - -0.5 70,908 - -0.1 71,452 + EX28DRAFT_3401 0.67 -1.6 71,452 + EX28DRAFT_3401 0.67 -1.2 71,644 + +0.8 71,646 + -1.0 71,646 + -0.3 71,692 + -2.1 71,693 - +0.2 72,050 + EX28DRAFT_3402 0.64 +0.8 72,051 - EX28DRAFT_3402 0.65 +0.4 72,051 - EX28DRAFT_3402 0.65 -0.2 72,089 + EX28DRAFT_3402 0.71 -0.7 72,103 + EX28DRAFT_3402 0.74 -0.0 72,104 - EX28DRAFT_3402 0.74 +0.0 72,104 - EX28DRAFT_3402 0.74 -0.1 72,108 - EX28DRAFT_3402 0.75 +0.2 72,114 - EX28DRAFT_3402 0.76 +0.6 72,233 + -0.2 72,233 + -0.3 72,237 - +0.5 72,237 - -0.4 72,240 + -0.7 72,299 + -0.0 72,299 + +0.6 72,300 - +1.2 72,300 - -0.4 72,300 - +0.2 72,305 + +0.4 72,305 + -0.0 72,306 - -0.6 72,364 + -0.3 72,364 + +0.2 72,365 - +0.0 72,388 + -0.7 72,454 - -0.3 72,474 + +0.7 72,485 + +0.4 72,485 + +0.6 72,486 - +0.3 72,486 - -0.4 72,488 - +0.8 72,551 - +0.1 72,567 - -0.3 72,591 + -0.4 72,592 - -0.7 72,592 - -0.2 72,615 + EX28DRAFT_3403 0.20 +0.1 72,616 - EX28DRAFT_3403 0.21 +0.2 72,645 + EX28DRAFT_3403 0.35 -0.1 72,645 + EX28DRAFT_3403 0.35 +0.2 72,645 + EX28DRAFT_3403 0.35 +0.1 72,646 - EX28DRAFT_3403 0.35 +1.2 72,646 - EX28DRAFT_3403 0.35 +0.3 72,646 - EX28DRAFT_3403 0.35 -0.2 72,646 - EX28DRAFT_3403 0.35 +0.1
Or see this region's nucleotide sequence