Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3279 and EX28DRAFT_3280 are separated by 15 nucleotides EX28DRAFT_3280 and EX28DRAFT_3281 are separated by 300 nucleotides EX28DRAFT_3281 and EX28DRAFT_3282 are separated by 189 nucleotides EX28DRAFT_3282 and EX28DRAFT_3283 are separated by 171 nucleotides
EX28DRAFT_3279: EX28DRAFT_3279 - Ribosomal protein S9, at 577,175 to 577,567
_3279
EX28DRAFT_3280: EX28DRAFT_3280 - LSU ribosomal protein L13P, at 577,583 to 578,011
_3280
EX28DRAFT_3281: EX28DRAFT_3281 - Predicted ATPase, at 578,312 to 579,436
_3281
EX28DRAFT_3282: EX28DRAFT_3282 - Uncharacterized protein conserved in bacteria, at 579,626 to 580,024
_3282
EX28DRAFT_3283: EX28DRAFT_3283 - periplasmic serine protease, Do/DeqQ family, at 580,196 to 581,563
_3283
Position (kb) 578
579
580 Strain fitness (log2 ratio) -2
-1
0
1 at 578.209 kb on + strand at 578.279 kb on + strand at 578.313 kb on + strand at 578.320 kb on + strand at 578.321 kb on - strand at 578.321 kb on - strand at 578.337 kb on + strand at 578.337 kb on + strand at 578.338 kb on - strand at 578.338 kb on - strand at 578.338 kb on - strand at 578.418 kb on + strand at 578.419 kb on - strand at 578.420 kb on + strand at 578.420 kb on + strand at 578.420 kb on + strand at 578.421 kb on - strand at 578.421 kb on - strand at 578.421 kb on - strand at 578.421 kb on - strand at 578.421 kb on - strand at 578.421 kb on - strand at 578.463 kb on + strand, within EX28DRAFT_3281 at 578.528 kb on + strand, within EX28DRAFT_3281 at 578.529 kb on - strand, within EX28DRAFT_3281 at 578.574 kb on + strand, within EX28DRAFT_3281 at 578.574 kb on + strand, within EX28DRAFT_3281 at 578.574 kb on + strand, within EX28DRAFT_3281 at 578.603 kb on + strand, within EX28DRAFT_3281 at 578.725 kb on + strand, within EX28DRAFT_3281 at 578.725 kb on + strand, within EX28DRAFT_3281 at 578.948 kb on + strand, within EX28DRAFT_3281 at 578.949 kb on - strand, within EX28DRAFT_3281 at 579.113 kb on - strand, within EX28DRAFT_3281 at 579.113 kb on - strand, within EX28DRAFT_3281 at 579.161 kb on - strand, within EX28DRAFT_3281 at 579.163 kb on - strand, within EX28DRAFT_3281 at 579.211 kb on + strand, within EX28DRAFT_3281 at 579.211 kb on + strand, within EX28DRAFT_3281 at 579.212 kb on - strand, within EX28DRAFT_3281 at 579.212 kb on - strand, within EX28DRAFT_3281 at 579.212 kb on - strand, within EX28DRAFT_3281 at 579.212 kb on - strand, within EX28DRAFT_3281 at 579.213 kb on + strand, within EX28DRAFT_3281 at 579.213 kb on + strand, within EX28DRAFT_3281 at 579.213 kb on + strand, within EX28DRAFT_3281 at 579.213 kb on + strand, within EX28DRAFT_3281 at 579.213 kb on + strand, within EX28DRAFT_3281 at 579.214 kb on - strand, within EX28DRAFT_3281 at 579.214 kb on - strand, within EX28DRAFT_3281 at 579.214 kb on - strand, within EX28DRAFT_3281 at 579.214 kb on - strand, within EX28DRAFT_3281 at 579.214 kb on - strand, within EX28DRAFT_3281 at 579.442 kb on + strand at 579.448 kb on + strand at 579.449 kb on - strand at 579.449 kb on - strand at 579.503 kb on + strand at 579.504 kb on - strand at 579.518 kb on + strand at 579.546 kb on - strand at 579.555 kb on - strand at 579.555 kb on - strand at 579.638 kb on + strand at 579.638 kb on + strand at 579.845 kb on + strand, within EX28DRAFT_3282 at 579.854 kb on - strand, within EX28DRAFT_3282 at 580.098 kb on - strand at 580.105 kb on + strand at 580.106 kb on - strand at 580.106 kb on - strand at 580.135 kb on + strand at 580.170 kb on - strand at 580.170 kb on - strand at 580.234 kb on - strand at 580.270 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 578,209 + +0.2 578,279 + -0.4 578,313 + -0.0 578,320 + +0.6 578,321 - -0.3 578,321 - +0.3 578,337 + -2.5 578,337 + -0.1 578,338 - +0.5 578,338 - -0.1 578,338 - -0.3 578,418 + +0.1 578,419 - -1.0 578,420 + -0.8 578,420 + -0.1 578,420 + -0.4 578,421 - -0.2 578,421 - -0.2 578,421 - -1.1 578,421 - +0.7 578,421 - -1.1 578,421 - -0.5 578,463 + EX28DRAFT_3281 0.13 -0.7 578,528 + EX28DRAFT_3281 0.19 -0.5 578,529 - EX28DRAFT_3281 0.19 -0.6 578,574 + EX28DRAFT_3281 0.23 -0.1 578,574 + EX28DRAFT_3281 0.23 -0.4 578,574 + EX28DRAFT_3281 0.23 -0.6 578,603 + EX28DRAFT_3281 0.26 +1.0 578,725 + EX28DRAFT_3281 0.37 -0.7 578,725 + EX28DRAFT_3281 0.37 -0.2 578,948 + EX28DRAFT_3281 0.57 -1.0 578,949 - EX28DRAFT_3281 0.57 -0.5 579,113 - EX28DRAFT_3281 0.71 -0.3 579,113 - EX28DRAFT_3281 0.71 -0.4 579,161 - EX28DRAFT_3281 0.75 -1.5 579,163 - EX28DRAFT_3281 0.76 -1.4 579,211 + EX28DRAFT_3281 0.80 -0.4 579,211 + EX28DRAFT_3281 0.80 -1.3 579,212 - EX28DRAFT_3281 0.80 -0.6 579,212 - EX28DRAFT_3281 0.80 -0.4 579,212 - EX28DRAFT_3281 0.80 +0.1 579,212 - EX28DRAFT_3281 0.80 -2.3 579,213 + EX28DRAFT_3281 0.80 -0.1 579,213 + EX28DRAFT_3281 0.80 -0.5 579,213 + EX28DRAFT_3281 0.80 -1.4 579,213 + EX28DRAFT_3281 0.80 -0.1 579,213 + EX28DRAFT_3281 0.80 -1.2 579,214 - EX28DRAFT_3281 0.80 -0.9 579,214 - EX28DRAFT_3281 0.80 +0.1 579,214 - EX28DRAFT_3281 0.80 -1.1 579,214 - EX28DRAFT_3281 0.80 -0.6 579,214 - EX28DRAFT_3281 0.80 -0.8 579,442 + +0.5 579,448 + -0.3 579,449 - +0.9 579,449 - +0.5 579,503 + -0.6 579,504 - -0.2 579,518 + -0.3 579,546 - -0.1 579,555 - -1.2 579,555 - -1.2 579,638 + +0.5 579,638 + -0.6 579,845 + EX28DRAFT_3282 0.55 +0.9 579,854 - EX28DRAFT_3282 0.57 +0.0 580,098 - -0.5 580,105 + -1.1 580,106 - +0.9 580,106 - +0.9 580,135 + -1.0 580,170 - -0.1 580,170 - +0.5 580,234 - -0.6 580,270 - -0.0
Or see this region's nucleotide sequence