Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3170 and EX28DRAFT_3171 are separated by 106 nucleotides EX28DRAFT_3171 and EX28DRAFT_3172 are separated by 46 nucleotides
EX28DRAFT_3170: EX28DRAFT_3170 - Predicted glutathione S-transferase, at 470,477 to 471,463
_3170
EX28DRAFT_3171: EX28DRAFT_3171 - Predicted membrane protein, at 471,570 to 471,935
_3171
EX28DRAFT_3172: EX28DRAFT_3172 - Transcriptional regulator, at 471,982 to 472,884
_3172
Position (kb) 471
472 Strain fitness (log2 ratio) -1
0
1
2
3 at 470.648 kb on + strand, within EX28DRAFT_3170 at 470.648 kb on + strand, within EX28DRAFT_3170 at 470.649 kb on - strand, within EX28DRAFT_3170 at 470.701 kb on + strand, within EX28DRAFT_3170 at 470.702 kb on - strand, within EX28DRAFT_3170 at 470.702 kb on - strand, within EX28DRAFT_3170 at 470.835 kb on - strand, within EX28DRAFT_3170 at 470.855 kb on + strand, within EX28DRAFT_3170 at 470.984 kb on + strand, within EX28DRAFT_3170 at 471.092 kb on + strand, within EX28DRAFT_3170 at 471.093 kb on - strand, within EX28DRAFT_3170 at 471.233 kb on + strand, within EX28DRAFT_3170 at 471.234 kb on - strand, within EX28DRAFT_3170 at 471.247 kb on + strand, within EX28DRAFT_3170 at 471.248 kb on - strand, within EX28DRAFT_3170 at 471.276 kb on - strand, within EX28DRAFT_3170 at 471.308 kb on + strand, within EX28DRAFT_3170 at 471.397 kb on + strand at 471.400 kb on + strand at 471.400 kb on + strand at 471.401 kb on - strand at 471.401 kb on - strand at 471.401 kb on - strand at 471.401 kb on - strand at 471.465 kb on + strand at 471.465 kb on + strand at 471.466 kb on - strand at 471.466 kb on - strand at 471.513 kb on - strand at 471.584 kb on - strand at 471.590 kb on - strand at 471.600 kb on + strand at 471.601 kb on - strand at 471.601 kb on - strand at 471.601 kb on - strand at 471.637 kb on - strand, within EX28DRAFT_3171 at 471.744 kb on + strand, within EX28DRAFT_3171 at 471.745 kb on - strand, within EX28DRAFT_3171 at 471.968 kb on + strand at 471.976 kb on - strand at 471.991 kb on - strand at 471.991 kb on - strand at 471.991 kb on - strand at 471.992 kb on + strand at 471.993 kb on - strand at 471.993 kb on - strand at 471.993 kb on - strand at 472.072 kb on - strand at 472.072 kb on - strand at 472.092 kb on - strand, within EX28DRAFT_3172 at 472.230 kb on + strand, within EX28DRAFT_3172 at 472.296 kb on - strand, within EX28DRAFT_3172 at 472.365 kb on - strand, within EX28DRAFT_3172 at 472.383 kb on - strand, within EX28DRAFT_3172 at 472.582 kb on + strand, within EX28DRAFT_3172 at 472.583 kb on - strand, within EX28DRAFT_3172 at 472.583 kb on - strand, within EX28DRAFT_3172 at 472.583 kb on - strand, within EX28DRAFT_3172 at 472.766 kb on + strand, within EX28DRAFT_3172 at 472.766 kb on + strand, within EX28DRAFT_3172 at 472.878 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 470,648 + EX28DRAFT_3170 0.17 +0.3 470,648 + EX28DRAFT_3170 0.17 +0.0 470,649 - EX28DRAFT_3170 0.17 +0.0 470,701 + EX28DRAFT_3170 0.23 -0.6 470,702 - EX28DRAFT_3170 0.23 -0.5 470,702 - EX28DRAFT_3170 0.23 +0.0 470,835 - EX28DRAFT_3170 0.36 -0.7 470,855 + EX28DRAFT_3170 0.38 -0.6 470,984 + EX28DRAFT_3170 0.51 -0.3 471,092 + EX28DRAFT_3170 0.62 +2.3 471,093 - EX28DRAFT_3170 0.62 -0.9 471,233 + EX28DRAFT_3170 0.77 -1.2 471,234 - EX28DRAFT_3170 0.77 -1.6 471,247 + EX28DRAFT_3170 0.78 +0.0 471,248 - EX28DRAFT_3170 0.78 -0.3 471,276 - EX28DRAFT_3170 0.81 +0.4 471,308 + EX28DRAFT_3170 0.84 +0.3 471,397 + -0.3 471,400 + +0.6 471,400 + -0.8 471,401 - +0.0 471,401 - -0.3 471,401 - -0.1 471,401 - +0.5 471,465 + -0.5 471,465 + -0.0 471,466 - -0.5 471,466 - +0.0 471,513 - -0.4 471,584 - +2.9 471,590 - +0.4 471,600 + +0.4 471,601 - -0.1 471,601 - +0.3 471,601 - +0.8 471,637 - EX28DRAFT_3171 0.18 -0.6 471,744 + EX28DRAFT_3171 0.48 +1.7 471,745 - EX28DRAFT_3171 0.48 -1.3 471,968 + +0.5 471,976 - -0.6 471,991 - +0.3 471,991 - -0.2 471,991 - -0.1 471,992 + +0.3 471,993 - +0.2 471,993 - +0.3 471,993 - +0.1 472,072 - -0.2 472,072 - +0.2 472,092 - EX28DRAFT_3172 0.12 +0.2 472,230 + EX28DRAFT_3172 0.27 +0.6 472,296 - EX28DRAFT_3172 0.35 -0.3 472,365 - EX28DRAFT_3172 0.42 -0.5 472,383 - EX28DRAFT_3172 0.44 -0.3 472,582 + EX28DRAFT_3172 0.66 -0.8 472,583 - EX28DRAFT_3172 0.67 -0.0 472,583 - EX28DRAFT_3172 0.67 +0.8 472,583 - EX28DRAFT_3172 0.67 +0.3 472,766 + EX28DRAFT_3172 0.87 -0.2 472,766 + EX28DRAFT_3172 0.87 +0.3 472,878 + +0.5
Or see this region's nucleotide sequence