Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2973 and EX28DRAFT_2974 are separated by 341 nucleotides EX28DRAFT_2974 and EX28DRAFT_2975 are separated by 224 nucleotides
EX28DRAFT_2973: EX28DRAFT_2973 - erythrose-4-phosphate dehydrogenase, at 282,874 to 283,893
_2973
EX28DRAFT_2974: EX28DRAFT_2974 - transketolase, bacterial and yeast, at 284,235 to 286,226
_2974
EX28DRAFT_2975: EX28DRAFT_2975 - Zn-dependent protease with chaperone function, at 286,451 to 287,257
_2975
Position (kb)
284
285
286
287 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 283.313 kb on + strand, within EX28DRAFT_2973 at 283.314 kb on - strand, within EX28DRAFT_2973 at 283.746 kb on - strand, within EX28DRAFT_2973 at 283.832 kb on - strand at 283.833 kb on - strand at 283.848 kb on + strand at 283.848 kb on + strand at 283.849 kb on - strand at 283.893 kb on + strand at 284.079 kb on - strand at 284.087 kb on + strand at 284.088 kb on - strand at 284.117 kb on + strand at 284.172 kb on + strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.226 kb on + strand at 284.227 kb on - strand at 284.236 kb on + strand at 284.237 kb on - strand at 284.362 kb on + strand at 284.363 kb on - strand at 284.938 kb on + strand, within EX28DRAFT_2974 at 284.966 kb on + strand, within EX28DRAFT_2974 at 284.966 kb on + strand, within EX28DRAFT_2974 at 285.000 kb on + strand, within EX28DRAFT_2974 at 285.017 kb on - strand, within EX28DRAFT_2974 at 285.030 kb on - strand, within EX28DRAFT_2974 at 285.047 kb on + strand, within EX28DRAFT_2974 at 285.331 kb on - strand, within EX28DRAFT_2974 at 285.682 kb on + strand, within EX28DRAFT_2974 at 285.792 kb on + strand, within EX28DRAFT_2974 at 285.792 kb on + strand, within EX28DRAFT_2974 at 285.793 kb on - strand, within EX28DRAFT_2974 at 285.941 kb on + strand, within EX28DRAFT_2974 at 286.010 kb on + strand, within EX28DRAFT_2974 at 286.137 kb on - strand at 286.215 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.332 kb on - strand at 286.423 kb on - strand at 286.423 kb on - strand at 286.423 kb on - strand at 286.423 kb on - strand at 286.427 kb on - strand at 286.468 kb on + strand at 286.469 kb on - strand at 286.469 kb on - strand at 286.477 kb on + strand at 286.515 kb on + strand at 286.657 kb on + strand, within EX28DRAFT_2975 at 286.658 kb on - strand, within EX28DRAFT_2975 at 286.664 kb on - strand, within EX28DRAFT_2975 at 286.699 kb on + strand, within EX28DRAFT_2975 at 286.699 kb on + strand, within EX28DRAFT_2975 at 286.700 kb on - strand, within EX28DRAFT_2975 at 286.700 kb on - strand, within EX28DRAFT_2975 at 286.704 kb on - strand, within EX28DRAFT_2975 at 286.778 kb on + strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.779 kb on - strand, within EX28DRAFT_2975 at 286.833 kb on - strand, within EX28DRAFT_2975 at 287.038 kb on + strand, within EX28DRAFT_2975 at 287.081 kb on + strand, within EX28DRAFT_2975 at 287.081 kb on + strand, within EX28DRAFT_2975 at 287.081 kb on + strand, within EX28DRAFT_2975 at 287.088 kb on - strand, within EX28DRAFT_2975 at 287.114 kb on - strand, within EX28DRAFT_2975 at 287.221 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 283,313 + EX28DRAFT_2973 0.43 -1.0 283,314 - EX28DRAFT_2973 0.43 -0.5 283,746 - EX28DRAFT_2973 0.85 -1.2 283,832 - -0.5 283,833 - +0.0 283,848 + -0.2 283,848 + +0.2 283,849 - +0.2 283,893 + +0.2 284,079 - +0.4 284,087 + +0.4 284,088 - +0.2 284,117 + +0.5 284,172 + +0.3 284,173 - +0.1 284,173 - +0.6 284,173 - -0.5 284,173 - +0.1 284,173 - -0.1 284,226 + -0.5 284,227 - +1.1 284,236 + -0.5 284,237 - -0.6 284,362 + +0.4 284,363 - +0.6 284,938 + EX28DRAFT_2974 0.35 -0.4 284,966 + EX28DRAFT_2974 0.37 -1.2 284,966 + EX28DRAFT_2974 0.37 -0.8 285,000 + EX28DRAFT_2974 0.38 -2.0 285,017 - EX28DRAFT_2974 0.39 -0.4 285,030 - EX28DRAFT_2974 0.40 -1.4 285,047 + EX28DRAFT_2974 0.41 -0.6 285,331 - EX28DRAFT_2974 0.55 -0.8 285,682 + EX28DRAFT_2974 0.73 -1.2 285,792 + EX28DRAFT_2974 0.78 -0.4 285,792 + EX28DRAFT_2974 0.78 -0.4 285,793 - EX28DRAFT_2974 0.78 -1.1 285,941 + EX28DRAFT_2974 0.86 -1.6 286,010 + EX28DRAFT_2974 0.89 -2.3 286,137 - -1.8 286,215 - -0.4 286,332 - +0.2 286,332 - -0.5 286,332 - +1.8 286,332 - -0.8 286,332 - -0.0 286,423 - -1.0 286,423 - +0.0 286,423 - -0.5 286,423 - -0.2 286,427 - -0.2 286,468 + -0.2 286,469 - +0.7 286,469 - -0.3 286,477 + -0.2 286,515 + +0.1 286,657 + EX28DRAFT_2975 0.26 +0.9 286,658 - EX28DRAFT_2975 0.26 -0.3 286,664 - EX28DRAFT_2975 0.26 +0.8 286,699 + EX28DRAFT_2975 0.31 +0.3 286,699 + EX28DRAFT_2975 0.31 +0.3 286,700 - EX28DRAFT_2975 0.31 -0.8 286,700 - EX28DRAFT_2975 0.31 -0.1 286,704 - EX28DRAFT_2975 0.31 -0.1 286,778 + EX28DRAFT_2975 0.41 +0.5 286,779 - EX28DRAFT_2975 0.41 -0.6 286,779 - EX28DRAFT_2975 0.41 -0.4 286,779 - EX28DRAFT_2975 0.41 +1.2 286,779 - EX28DRAFT_2975 0.41 -0.4 286,779 - EX28DRAFT_2975 0.41 +0.6 286,833 - EX28DRAFT_2975 0.47 +0.5 287,038 + EX28DRAFT_2975 0.73 -0.7 287,081 + EX28DRAFT_2975 0.78 -0.4 287,081 + EX28DRAFT_2975 0.78 -0.8 287,081 + EX28DRAFT_2975 0.78 +0.0 287,088 - EX28DRAFT_2975 0.79 +0.4 287,114 - EX28DRAFT_2975 0.82 +1.2 287,221 + +0.6
Or see this region's nucleotide sequence