Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2955 and EX28DRAFT_2956 are separated by 124 nucleotides EX28DRAFT_2956 and EX28DRAFT_2957 are separated by 23 nucleotides EX28DRAFT_2957 and EX28DRAFT_2958 are separated by 397 nucleotides
EX28DRAFT_2955: EX28DRAFT_2955 - glycine dehydrogenase (decarboxylating), at 263,937 to 266,810
_2955
EX28DRAFT_2956: EX28DRAFT_2956 - glycine cleavage system H protein, at 266,935 to 267,324
_2956
EX28DRAFT_2957: EX28DRAFT_2957 - glycine cleavage system T protein, at 267,348 to 268,442
_2957
EX28DRAFT_2958: EX28DRAFT_2958 - Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family, at 268,840 to 270,042
_2958
Position (kb) 267
268
269 Strain fitness (log2 ratio) -2
-1
0
1 at 266.477 kb on - strand, within EX28DRAFT_2955 at 266.477 kb on - strand, within EX28DRAFT_2955 at 266.561 kb on - strand at 266.567 kb on + strand at 266.567 kb on + strand at 266.567 kb on + strand at 266.698 kb on - strand at 266.797 kb on - strand at 266.843 kb on - strand at 266.843 kb on - strand at 266.929 kb on - strand at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.251 kb on + strand, within EX28DRAFT_2956 at 267.251 kb on + strand, within EX28DRAFT_2956 at 267.736 kb on + strand, within EX28DRAFT_2957 at 267.767 kb on + strand, within EX28DRAFT_2957 at 267.878 kb on - strand, within EX28DRAFT_2957 at 268.048 kb on + strand, within EX28DRAFT_2957 at 268.136 kb on - strand, within EX28DRAFT_2957 at 268.976 kb on - strand, within EX28DRAFT_2958
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 266,477 - EX28DRAFT_2955 0.88 -1.3 266,477 - EX28DRAFT_2955 0.88 -0.8 266,561 - -0.8 266,567 + +0.4 266,567 + -0.3 266,567 + -0.5 266,698 - +0.6 266,797 - -1.4 266,843 - -0.2 266,843 - +0.7 266,929 - +1.6 267,113 + EX28DRAFT_2956 0.46 -1.0 267,113 + EX28DRAFT_2956 0.46 -0.5 267,113 + EX28DRAFT_2956 0.46 -0.0 267,113 + EX28DRAFT_2956 0.46 -0.0 267,113 + EX28DRAFT_2956 0.46 -0.3 267,113 + EX28DRAFT_2956 0.46 -1.1 267,113 + EX28DRAFT_2956 0.46 -0.3 267,114 - EX28DRAFT_2956 0.46 +0.3 267,114 - EX28DRAFT_2956 0.46 -0.4 267,114 - EX28DRAFT_2956 0.46 -0.1 267,114 - EX28DRAFT_2956 0.46 -0.4 267,251 + EX28DRAFT_2956 0.81 +1.2 267,251 + EX28DRAFT_2956 0.81 +0.6 267,736 + EX28DRAFT_2957 0.35 -1.3 267,767 + EX28DRAFT_2957 0.38 -1.0 267,878 - EX28DRAFT_2957 0.48 +1.2 268,048 + EX28DRAFT_2957 0.64 -2.0 268,136 - EX28DRAFT_2957 0.72 +0.6 268,976 - EX28DRAFT_2958 0.11 -1.0
Or see this region's nucleotide sequence