Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2943 and EX28DRAFT_2944 are separated by 5 nucleotides EX28DRAFT_2944 and EX28DRAFT_2945 are separated by 28 nucleotides EX28DRAFT_2945 and EX28DRAFT_2946 are separated by 101 nucleotides EX28DRAFT_2946 and EX28DRAFT_2947 are separated by 6 nucleotides
EX28DRAFT_2943: EX28DRAFT_2943 - single-stranded-DNA-specific exonuclease RecJ, at 253,527 to 255,260
_2943
EX28DRAFT_2944: EX28DRAFT_2944 - Protein-disulfide isomerase, at 255,266 to 255,979
_2944
EX28DRAFT_2945: EX28DRAFT_2945 - tyrosine recombinase XerD, at 256,008 to 256,904
_2945
EX28DRAFT_2946: EX28DRAFT_2946 - flavodoxin, long chain, at 257,006 to 257,527
_2946
EX28DRAFT_2947: EX28DRAFT_2947 - Protein of unknown function (DUF1434), at 257,534 to 257,944
_2947
Position (kb) 256
257 Strain fitness (log2 ratio) -2
-1
0
1 at 255.268 kb on - strand at 255.272 kb on - strand at 255.503 kb on - strand, within EX28DRAFT_2944 at 255.585 kb on + strand, within EX28DRAFT_2944 at 255.673 kb on + strand, within EX28DRAFT_2944 at 255.763 kb on - strand, within EX28DRAFT_2944 at 255.934 kb on + strand at 255.979 kb on + strand at 256.170 kb on + strand, within EX28DRAFT_2945 at 256.477 kb on + strand, within EX28DRAFT_2945 at 256.478 kb on - strand, within EX28DRAFT_2945 at 256.543 kb on - strand, within EX28DRAFT_2945 at 256.936 kb on + strand at 256.996 kb on + strand at 257.001 kb on - strand at 257.011 kb on + strand at 257.012 kb on - strand at 257.025 kb on - strand at 257.141 kb on + strand, within EX28DRAFT_2946 at 257.142 kb on - strand, within EX28DRAFT_2946 at 257.187 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.357 kb on + strand, within EX28DRAFT_2946 at 257.597 kb on - strand, within EX28DRAFT_2947 at 257.738 kb on + strand, within EX28DRAFT_2947 at 257.744 kb on - strand, within EX28DRAFT_2947 at 257.830 kb on + strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.888 kb on + strand, within EX28DRAFT_2947
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 255,268 - -1.1 255,272 - +1.4 255,503 - EX28DRAFT_2944 0.33 -0.5 255,585 + EX28DRAFT_2944 0.45 -0.4 255,673 + EX28DRAFT_2944 0.57 -0.1 255,763 - EX28DRAFT_2944 0.70 +0.6 255,934 + -0.1 255,979 + -0.0 256,170 + EX28DRAFT_2945 0.18 +0.2 256,477 + EX28DRAFT_2945 0.52 +1.2 256,478 - EX28DRAFT_2945 0.52 -0.8 256,543 - EX28DRAFT_2945 0.60 -2.7 256,936 + -0.1 256,996 + +0.9 257,001 - +0.3 257,011 + -0.9 257,012 - -0.2 257,025 - -0.6 257,141 + EX28DRAFT_2946 0.26 +0.5 257,142 - EX28DRAFT_2946 0.26 -0.1 257,187 + EX28DRAFT_2946 0.35 -0.6 257,206 + EX28DRAFT_2946 0.38 -0.5 257,206 + EX28DRAFT_2946 0.38 +0.4 257,357 + EX28DRAFT_2946 0.67 +0.6 257,597 - EX28DRAFT_2947 0.15 -0.3 257,738 + EX28DRAFT_2947 0.50 -0.2 257,744 - EX28DRAFT_2947 0.51 +0.6 257,830 + EX28DRAFT_2947 0.72 -0.0 257,831 - EX28DRAFT_2947 0.72 +0.4 257,831 - EX28DRAFT_2947 0.72 -0.4 257,831 - EX28DRAFT_2947 0.72 +0.5 257,831 - EX28DRAFT_2947 0.72 +0.6 257,833 - EX28DRAFT_2947 0.73 -0.3 257,833 - EX28DRAFT_2947 0.73 -0.8 257,888 + EX28DRAFT_2947 0.86 -0.0
Or see this region's nucleotide sequence