Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2824 and EX28DRAFT_2825 overlap by 1 nucleotides EX28DRAFT_2825 and EX28DRAFT_2826 are separated by 16 nucleotides EX28DRAFT_2826 and EX28DRAFT_2827 are separated by 5 nucleotides EX28DRAFT_2827 and EX28DRAFT_2828 are separated by 175 nucleotides EX28DRAFT_2828 and EX28DRAFT_2829 are separated by 113 nucleotides
EX28DRAFT_2824: EX28DRAFT_2824 - ABC-type Fe3+-siderophore transport system, permease component, at 127,709 to 128,746
_2824
EX28DRAFT_2825: EX28DRAFT_2825 - ABC-type cobalamin/Fe3+-siderophores transport systems, ATPase components, at 128,746 to 129,507
_2825
EX28DRAFT_2826: EX28DRAFT_2826 - Major Facilitator Superfamily, at 129,524 to 130,744
_2826
EX28DRAFT_2827: EX28DRAFT_2827 - Nitrous oxide-stimulated promoter, at 130,750 to 131,094
_2827
EX28DRAFT_2828: EX28DRAFT_2828 - Toxin SymE, type I toxin-antitoxin system, at 131,270 to 131,500
_2828
EX28DRAFT_2829: EX28DRAFT_2829 - DNA mismatch repair protein MutS, at 131,614 to 134,175
_2829
Position (kb) 129
130
131 Strain fitness (log2 ratio) -2
-1
0
1 at 128.722 kb on + strand at 128.722 kb on + strand at 128.723 kb on - strand at 128.723 kb on - strand at 128.741 kb on - strand at 128.781 kb on - strand at 128.866 kb on - strand, within EX28DRAFT_2825 at 129.135 kb on + strand, within EX28DRAFT_2825 at 129.136 kb on - strand, within EX28DRAFT_2825 at 129.189 kb on + strand, within EX28DRAFT_2825 at 129.409 kb on - strand, within EX28DRAFT_2825 at 129.414 kb on - strand, within EX28DRAFT_2825 at 129.564 kb on + strand at 129.600 kb on - strand at 129.826 kb on + strand, within EX28DRAFT_2826 at 130.056 kb on + strand, within EX28DRAFT_2826 at 130.057 kb on - strand, within EX28DRAFT_2826 at 130.082 kb on + strand, within EX28DRAFT_2826 at 130.175 kb on - strand, within EX28DRAFT_2826 at 130.185 kb on - strand, within EX28DRAFT_2826 at 130.205 kb on + strand, within EX28DRAFT_2826 at 130.311 kb on + strand, within EX28DRAFT_2826 at 130.311 kb on + strand, within EX28DRAFT_2826 at 130.312 kb on - strand, within EX28DRAFT_2826 at 130.354 kb on - strand, within EX28DRAFT_2826 at 130.521 kb on + strand, within EX28DRAFT_2826 at 130.521 kb on + strand, within EX28DRAFT_2826 at 130.521 kb on + strand, within EX28DRAFT_2826 at 130.665 kb on - strand at 130.766 kb on + strand at 130.766 kb on + strand at 130.845 kb on + strand, within EX28DRAFT_2827 at 130.846 kb on - strand, within EX28DRAFT_2827 at 130.957 kb on - strand, within EX28DRAFT_2827 at 130.957 kb on - strand, within EX28DRAFT_2827 at 131.036 kb on + strand, within EX28DRAFT_2827 at 131.037 kb on - strand, within EX28DRAFT_2827 at 131.223 kb on + strand at 131.269 kb on + strand at 131.298 kb on - strand, within EX28DRAFT_2828 at 131.319 kb on - strand, within EX28DRAFT_2828 at 131.392 kb on - strand, within EX28DRAFT_2828 at 131.423 kb on - strand, within EX28DRAFT_2828 at 131.478 kb on + strand at 131.546 kb on - strand at 131.546 kb on - strand at 131.607 kb on + strand at 131.607 kb on + strand at 131.608 kb on - strand at 131.663 kb on - strand at 131.729 kb on + strand at 131.729 kb on + strand at 131.730 kb on - strand at 131.730 kb on - strand at 131.730 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 128,722 + -0.7 128,722 + -0.4 128,723 - +0.7 128,723 - +0.6 128,741 - -2.2 128,781 - +0.4 128,866 - EX28DRAFT_2825 0.16 -0.2 129,135 + EX28DRAFT_2825 0.51 -0.3 129,136 - EX28DRAFT_2825 0.51 -0.3 129,189 + EX28DRAFT_2825 0.58 +0.4 129,409 - EX28DRAFT_2825 0.87 -0.8 129,414 - EX28DRAFT_2825 0.88 -0.4 129,564 + -0.5 129,600 - -0.4 129,826 + EX28DRAFT_2826 0.25 +0.3 130,056 + EX28DRAFT_2826 0.44 -0.2 130,057 - EX28DRAFT_2826 0.44 +0.3 130,082 + EX28DRAFT_2826 0.46 -0.4 130,175 - EX28DRAFT_2826 0.53 -0.7 130,185 - EX28DRAFT_2826 0.54 -0.2 130,205 + EX28DRAFT_2826 0.56 -0.1 130,311 + EX28DRAFT_2826 0.64 +0.2 130,311 + EX28DRAFT_2826 0.64 -2.3 130,312 - EX28DRAFT_2826 0.65 -0.4 130,354 - EX28DRAFT_2826 0.68 -0.6 130,521 + EX28DRAFT_2826 0.82 +1.7 130,521 + EX28DRAFT_2826 0.82 +1.1 130,521 + EX28DRAFT_2826 0.82 -0.1 130,665 - -0.0 130,766 + +0.4 130,766 + +1.0 130,845 + EX28DRAFT_2827 0.28 +0.4 130,846 - EX28DRAFT_2827 0.28 +0.6 130,957 - EX28DRAFT_2827 0.60 +0.8 130,957 - EX28DRAFT_2827 0.60 -0.4 131,036 + EX28DRAFT_2827 0.83 +0.4 131,037 - EX28DRAFT_2827 0.83 +0.5 131,223 + -0.4 131,269 + -0.6 131,298 - EX28DRAFT_2828 0.12 +0.5 131,319 - EX28DRAFT_2828 0.21 +0.4 131,392 - EX28DRAFT_2828 0.53 -0.1 131,423 - EX28DRAFT_2828 0.66 +1.7 131,478 + +0.1 131,546 - -0.6 131,546 - +0.1 131,607 + +0.4 131,607 + -0.4 131,608 - +0.4 131,663 - -0.2 131,729 + -0.0 131,729 + -1.1 131,730 - +0.1 131,730 - -1.2 131,730 - -0.5
Or see this region's nucleotide sequence