Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2719 and EX28DRAFT_2720 are separated by 138 nucleotides EX28DRAFT_2720 and EX28DRAFT_2721 are separated by 103 nucleotides EX28DRAFT_2721 and EX28DRAFT_2722 overlap by 11 nucleotides EX28DRAFT_2722 and EX28DRAFT_2723 are separated by 156 nucleotides
EX28DRAFT_2719: EX28DRAFT_2719 - DNA repair protein RecN, at 23,359 to 25,020
_2719
EX28DRAFT_2720: EX28DRAFT_2720 - Small protein A (tmRNA-binding), at 25,159 to 25,497
_2720
EX28DRAFT_2721: EX28DRAFT_2721 - Uncharacterized protein conserved in bacteria, at 25,601 to 25,888
_2721
EX28DRAFT_2722: EX28DRAFT_2722 - Oligoketide cyclase/lipid transport protein, at 25,878 to 26,315
_2722
EX28DRAFT_2723: EX28DRAFT_2723 - SsrA-binding protein, at 26,472 to 26,954
_2723
Position (kb)
25
26 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 24.834 kb on - strand, within EX28DRAFT_2719 at 25.045 kb on - strand at 25.094 kb on + strand at 25.095 kb on - strand at 25.108 kb on + strand at 25.109 kb on - strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.111 kb on + strand at 25.112 kb on - strand at 25.115 kb on + strand at 25.116 kb on - strand at 25.116 kb on - strand at 25.267 kb on + strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.268 kb on - strand, within EX28DRAFT_2720 at 25.330 kb on - strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.381 kb on + strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.382 kb on - strand, within EX28DRAFT_2720 at 25.385 kb on + strand, within EX28DRAFT_2720 at 25.385 kb on + strand, within EX28DRAFT_2720 at 25.386 kb on - strand, within EX28DRAFT_2720 at 25.495 kb on + strand at 25.496 kb on - strand at 25.602 kb on + strand at 25.602 kb on + strand at 25.603 kb on - strand at 25.606 kb on + strand at 25.837 kb on - strand, within EX28DRAFT_2721 at 25.858 kb on - strand, within EX28DRAFT_2721 at 25.863 kb on - strand at 25.863 kb on - strand at 25.868 kb on + strand at 25.868 kb on + strand at 25.891 kb on + strand at 26.350 kb on - strand at 26.356 kb on + strand at 26.356 kb on + strand at 26.357 kb on - strand at 26.394 kb on + strand at 26.399 kb on + strand at 26.472 kb on - strand at 26.634 kb on + strand, within EX28DRAFT_2723 at 26.634 kb on + strand, within EX28DRAFT_2723 at 26.710 kb on - strand, within EX28DRAFT_2723
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 24,834 - EX28DRAFT_2719 0.89 -0.2 25,045 - -1.0 25,094 + -0.1 25,095 - -0.6 25,108 + -0.1 25,109 - -0.6 25,111 + -0.4 25,111 + -0.5 25,111 + -0.6 25,111 + +0.1 25,111 + +0.3 25,112 - -0.3 25,115 + +2.7 25,116 - +0.3 25,116 - -1.1 25,267 + EX28DRAFT_2720 0.32 -0.4 25,268 - EX28DRAFT_2720 0.32 -0.2 25,268 - EX28DRAFT_2720 0.32 +0.8 25,268 - EX28DRAFT_2720 0.32 -1.1 25,268 - EX28DRAFT_2720 0.32 -1.1 25,330 - EX28DRAFT_2720 0.50 -0.9 25,381 + EX28DRAFT_2720 0.65 +0.7 25,381 + EX28DRAFT_2720 0.65 -0.1 25,381 + EX28DRAFT_2720 0.65 +2.7 25,381 + EX28DRAFT_2720 0.65 -0.1 25,382 - EX28DRAFT_2720 0.66 -0.4 25,382 - EX28DRAFT_2720 0.66 +0.6 25,382 - EX28DRAFT_2720 0.66 +1.9 25,382 - EX28DRAFT_2720 0.66 +0.1 25,385 + EX28DRAFT_2720 0.67 +0.2 25,385 + EX28DRAFT_2720 0.67 -0.0 25,386 - EX28DRAFT_2720 0.67 +0.1 25,495 + -0.4 25,496 - -0.9 25,602 + -0.7 25,602 + -2.4 25,603 - -0.7 25,606 + -0.2 25,837 - EX28DRAFT_2721 0.82 -1.9 25,858 - EX28DRAFT_2721 0.89 -0.6 25,863 - -0.2 25,863 - -2.3 25,868 + +0.3 25,868 + -0.3 25,891 + -1.0 26,350 - +0.8 26,356 + -0.0 26,356 + +1.5 26,357 - -0.0 26,394 + +0.2 26,399 + +0.2 26,472 - +3.1 26,634 + EX28DRAFT_2723 0.34 -0.0 26,634 + EX28DRAFT_2723 0.34 -1.8 26,710 - EX28DRAFT_2723 0.49 -0.2
Or see this region's nucleotide sequence