Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2621 and EX28DRAFT_2622 are separated by 576 nucleotides EX28DRAFT_2622 and EX28DRAFT_2623 are separated by 259 nucleotides
EX28DRAFT_2621: EX28DRAFT_2621 - hypothetical protein, at 2,660,141 to 2,660,500
_2621
EX28DRAFT_2622: EX28DRAFT_2622 - hypothetical protein, at 2,661,077 to 2,661,432
_2622
EX28DRAFT_2623: EX28DRAFT_2623 - Enolase, at 2,661,692 to 2,662,963
_2623
Position (kb)
2661
2662 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2660.079 kb on - strand at 2660.129 kb on + strand at 2660.138 kb on + strand at 2660.139 kb on - strand at 2660.139 kb on - strand at 2660.223 kb on + strand, within EX28DRAFT_2621 at 2660.223 kb on + strand, within EX28DRAFT_2621 at 2660.353 kb on - strand, within EX28DRAFT_2621 at 2660.449 kb on - strand, within EX28DRAFT_2621 at 2660.515 kb on + strand at 2660.516 kb on - strand at 2660.592 kb on + strand at 2660.690 kb on + strand at 2660.691 kb on - strand at 2660.725 kb on + strand at 2660.725 kb on + strand at 2660.725 kb on + strand at 2660.726 kb on - strand at 2660.791 kb on + strand at 2660.872 kb on + strand at 2660.872 kb on + strand at 2660.928 kb on + strand at 2660.935 kb on + strand at 2660.935 kb on + strand at 2660.935 kb on + strand at 2660.988 kb on + strand at 2660.988 kb on + strand at 2660.999 kb on + strand at 2660.999 kb on + strand at 2661.000 kb on - strand at 2661.051 kb on - strand at 2661.051 kb on - strand at 2661.172 kb on + strand, within EX28DRAFT_2622 at 2661.231 kb on + strand, within EX28DRAFT_2622 at 2661.251 kb on + strand, within EX28DRAFT_2622 at 2661.251 kb on + strand, within EX28DRAFT_2622 at 2661.290 kb on + strand, within EX28DRAFT_2622 at 2661.294 kb on - strand, within EX28DRAFT_2622 at 2661.328 kb on + strand, within EX28DRAFT_2622 at 2661.328 kb on + strand, within EX28DRAFT_2622 at 2661.329 kb on - strand, within EX28DRAFT_2622 at 2661.455 kb on + strand at 2661.498 kb on + strand at 2661.498 kb on + strand at 2661.568 kb on + strand at 2661.569 kb on - strand at 2661.590 kb on + strand at 2661.591 kb on - strand at 2661.591 kb on - strand at 2661.591 kb on - strand at 2661.593 kb on + strand at 2661.594 kb on - strand at 2661.594 kb on - strand at 2661.631 kb on + strand at 2661.631 kb on + strand at 2661.631 kb on + strand at 2661.631 kb on + strand at 2661.632 kb on - strand at 2661.632 kb on - strand at 2661.632 kb on - strand at 2661.635 kb on + strand at 2661.635 kb on + strand at 2661.635 kb on + strand at 2661.635 kb on + strand at 2661.636 kb on - strand at 2661.636 kb on - strand at 2661.684 kb on + strand at 2661.685 kb on - strand at 2661.719 kb on + strand at 2661.719 kb on + strand at 2661.720 kb on - strand at 2661.978 kb on - strand, within EX28DRAFT_2623 at 2661.978 kb on - strand, within EX28DRAFT_2623 at 2662.072 kb on + strand, within EX28DRAFT_2623 at 2662.072 kb on + strand, within EX28DRAFT_2623 at 2662.072 kb on + strand, within EX28DRAFT_2623 at 2662.161 kb on + strand, within EX28DRAFT_2623 at 2662.161 kb on + strand, within EX28DRAFT_2623 at 2662.162 kb on - strand, within EX28DRAFT_2623 at 2662.162 kb on - strand, within EX28DRAFT_2623 at 2662.162 kb on - strand, within EX28DRAFT_2623 at 2662.259 kb on - strand, within EX28DRAFT_2623 at 2662.259 kb on - strand, within EX28DRAFT_2623 at 2662.264 kb on + strand, within EX28DRAFT_2623 at 2662.301 kb on + strand, within EX28DRAFT_2623
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,660,079 - -0.1 2,660,129 + +0.3 2,660,138 + +0.2 2,660,139 - +1.3 2,660,139 - +1.2 2,660,223 + EX28DRAFT_2621 0.23 -0.4 2,660,223 + EX28DRAFT_2621 0.23 +0.7 2,660,353 - EX28DRAFT_2621 0.59 -0.1 2,660,449 - EX28DRAFT_2621 0.86 +0.3 2,660,515 + -0.0 2,660,516 - -0.4 2,660,592 + +0.1 2,660,690 + +0.2 2,660,691 - +0.3 2,660,725 + +0.3 2,660,725 + +0.7 2,660,725 + +0.3 2,660,726 - -0.8 2,660,791 + -0.3 2,660,872 + -0.4 2,660,872 + -0.1 2,660,928 + +0.9 2,660,935 + -0.7 2,660,935 + +0.2 2,660,935 + +0.6 2,660,988 + -0.1 2,660,988 + -0.3 2,660,999 + +0.6 2,660,999 + +0.5 2,661,000 - -0.1 2,661,051 - +2.0 2,661,051 - +1.0 2,661,172 + EX28DRAFT_2622 0.27 -0.3 2,661,231 + EX28DRAFT_2622 0.43 +0.1 2,661,251 + EX28DRAFT_2622 0.49 -0.5 2,661,251 + EX28DRAFT_2622 0.49 -0.0 2,661,290 + EX28DRAFT_2622 0.60 +0.9 2,661,294 - EX28DRAFT_2622 0.61 -2.2 2,661,328 + EX28DRAFT_2622 0.71 -0.2 2,661,328 + EX28DRAFT_2622 0.71 -1.0 2,661,329 - EX28DRAFT_2622 0.71 +0.1 2,661,455 + -0.2 2,661,498 + +0.2 2,661,498 + -0.5 2,661,568 + -0.2 2,661,569 - -0.5 2,661,590 + -0.8 2,661,591 - -0.2 2,661,591 - +0.0 2,661,591 - +0.1 2,661,593 + -0.0 2,661,594 - +1.0 2,661,594 - -0.0 2,661,631 + +1.0 2,661,631 + -0.0 2,661,631 + +0.7 2,661,631 + +1.4 2,661,632 - +0.2 2,661,632 - +0.1 2,661,632 - -1.7 2,661,635 + +0.5 2,661,635 + +0.5 2,661,635 + +0.1 2,661,635 + -0.7 2,661,636 - -0.8 2,661,636 - -0.1 2,661,684 + +0.4 2,661,685 - +0.8 2,661,719 + +0.5 2,661,719 + +0.6 2,661,720 - -0.5 2,661,978 - EX28DRAFT_2623 0.22 +0.4 2,661,978 - EX28DRAFT_2623 0.22 -0.8 2,662,072 + EX28DRAFT_2623 0.30 +1.0 2,662,072 + EX28DRAFT_2623 0.30 +0.4 2,662,072 + EX28DRAFT_2623 0.30 -0.6 2,662,161 + EX28DRAFT_2623 0.37 +0.1 2,662,161 + EX28DRAFT_2623 0.37 +0.9 2,662,162 - EX28DRAFT_2623 0.37 +0.5 2,662,162 - EX28DRAFT_2623 0.37 +2.0 2,662,162 - EX28DRAFT_2623 0.37 +0.1 2,662,259 - EX28DRAFT_2623 0.45 +0.3 2,662,259 - EX28DRAFT_2623 0.45 +0.2 2,662,264 + EX28DRAFT_2623 0.45 -0.5 2,662,301 + EX28DRAFT_2623 0.48 -0.5
Or see this region's nucleotide sequence