Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2511 and EX28DRAFT_2512 are separated by 81 nucleotides EX28DRAFT_2512 and EX28DRAFT_2513 overlap by 4 nucleotides EX28DRAFT_2513 and EX28DRAFT_2514 are separated by 261 nucleotides EX28DRAFT_2514 and EX28DRAFT_2515 are separated by 170 nucleotides
EX28DRAFT_2511: EX28DRAFT_2511 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 2,543,295 to 2,544,716
_2511
EX28DRAFT_2512: EX28DRAFT_2512 - hypothetical protein, at 2,544,798 to 2,545,013
_2512
EX28DRAFT_2513: EX28DRAFT_2513 - hypothetical protein, at 2,545,010 to 2,545,261
_2513
EX28DRAFT_2514: EX28DRAFT_2514 - RNA, at 2,545,523 to 2,545,619
_2514
EX28DRAFT_2515: EX28DRAFT_2515 - O-6-methylguanine DNA methyltransferase, at 2,545,790 to 2,546,101
_2515
Position (kb)
2544
2545
2546 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2544.056 kb on + strand, within EX28DRAFT_2511 at 2544.209 kb on - strand, within EX28DRAFT_2511 at 2544.357 kb on - strand, within EX28DRAFT_2511 at 2544.539 kb on - strand, within EX28DRAFT_2511 at 2544.601 kb on + strand at 2544.601 kb on + strand at 2544.601 kb on + strand at 2544.601 kb on + strand at 2544.605 kb on + strand at 2544.714 kb on + strand at 2544.725 kb on + strand at 2544.767 kb on + strand at 2544.800 kb on - strand at 2544.816 kb on - strand at 2544.936 kb on + strand, within EX28DRAFT_2512 at 2544.936 kb on + strand, within EX28DRAFT_2512 at 2544.937 kb on - strand, within EX28DRAFT_2512 at 2544.949 kb on + strand, within EX28DRAFT_2512 at 2544.982 kb on + strand, within EX28DRAFT_2512 at 2544.982 kb on + strand, within EX28DRAFT_2512 at 2545.130 kb on + strand, within EX28DRAFT_2513 at 2545.130 kb on + strand, within EX28DRAFT_2513 at 2545.287 kb on + strand at 2545.326 kb on + strand at 2545.354 kb on - strand at 2545.354 kb on - strand at 2545.355 kb on + strand at 2545.430 kb on - strand at 2545.784 kb on - strand at 2545.789 kb on + strand at 2545.822 kb on - strand, within EX28DRAFT_2515 at 2545.866 kb on - strand, within EX28DRAFT_2515 at 2545.866 kb on - strand, within EX28DRAFT_2515 at 2545.946 kb on - strand, within EX28DRAFT_2515
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,544,056 + EX28DRAFT_2511 0.54 +0.2 2,544,209 - EX28DRAFT_2511 0.64 -0.7 2,544,357 - EX28DRAFT_2511 0.75 +0.5 2,544,539 - EX28DRAFT_2511 0.87 +0.4 2,544,601 + +0.1 2,544,601 + +0.5 2,544,601 + +0.1 2,544,601 + +0.2 2,544,605 + -0.4 2,544,714 + -0.8 2,544,725 + +1.1 2,544,767 + +1.1 2,544,800 - +0.7 2,544,816 - +0.2 2,544,936 + EX28DRAFT_2512 0.64 -0.7 2,544,936 + EX28DRAFT_2512 0.64 -0.3 2,544,937 - EX28DRAFT_2512 0.64 -2.1 2,544,949 + EX28DRAFT_2512 0.70 -0.7 2,544,982 + EX28DRAFT_2512 0.85 -1.5 2,544,982 + EX28DRAFT_2512 0.85 -0.9 2,545,130 + EX28DRAFT_2513 0.48 -0.3 2,545,130 + EX28DRAFT_2513 0.48 +0.4 2,545,287 + +0.4 2,545,326 + +4.4 2,545,354 - +0.0 2,545,354 - +0.1 2,545,355 + +0.0 2,545,430 - -0.3 2,545,784 - +0.1 2,545,789 + -0.8 2,545,822 - EX28DRAFT_2515 0.10 +1.1 2,545,866 - EX28DRAFT_2515 0.24 +0.2 2,545,866 - EX28DRAFT_2515 0.24 -0.6 2,545,946 - EX28DRAFT_2515 0.50 +0.2
Or see this region's nucleotide sequence