Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2508 and EX28DRAFT_2509 are separated by 102 nucleotides EX28DRAFT_2509 and EX28DRAFT_2510 are separated by 77 nucleotides EX28DRAFT_2510 and EX28DRAFT_2511 are separated by 86 nucleotides
EX28DRAFT_2508: EX28DRAFT_2508 - Beta-galactosidase/beta-glucuronidase, at 2,538,257 to 2,541,346
_2508
EX28DRAFT_2509: EX28DRAFT_2509 - transcriptional regulator, LacI family, at 2,541,449 to 2,542,519
_2509
EX28DRAFT_2510: EX28DRAFT_2510 - Predicted membrane protein, at 2,542,597 to 2,543,208
_2510
EX28DRAFT_2511: EX28DRAFT_2511 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 2,543,295 to 2,544,716
_2511
Position (kb)
2541
2542
2543 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2540.692 kb on + strand, within EX28DRAFT_2508 at 2540.754 kb on + strand, within EX28DRAFT_2508 at 2540.754 kb on + strand, within EX28DRAFT_2508 at 2540.755 kb on - strand, within EX28DRAFT_2508 at 2540.778 kb on - strand, within EX28DRAFT_2508 at 2540.778 kb on - strand, within EX28DRAFT_2508 at 2540.778 kb on - strand, within EX28DRAFT_2508 at 2541.022 kb on + strand, within EX28DRAFT_2508 at 2541.043 kb on - strand at 2541.157 kb on - strand at 2541.157 kb on - strand at 2541.157 kb on - strand at 2541.161 kb on - strand at 2541.349 kb on + strand at 2541.408 kb on - strand at 2541.526 kb on + strand at 2541.527 kb on - strand at 2541.557 kb on - strand, within EX28DRAFT_2509 at 2541.877 kb on - strand, within EX28DRAFT_2509 at 2542.381 kb on - strand, within EX28DRAFT_2509 at 2542.500 kb on + strand at 2542.501 kb on - strand at 2542.501 kb on - strand at 2542.550 kb on + strand at 2542.672 kb on - strand, within EX28DRAFT_2510 at 2542.672 kb on - strand, within EX28DRAFT_2510 at 2542.694 kb on + strand, within EX28DRAFT_2510 at 2542.694 kb on + strand, within EX28DRAFT_2510 at 2542.695 kb on - strand, within EX28DRAFT_2510 at 2542.695 kb on - strand, within EX28DRAFT_2510 at 2542.695 kb on - strand, within EX28DRAFT_2510 at 2542.856 kb on + strand, within EX28DRAFT_2510 at 2542.856 kb on + strand, within EX28DRAFT_2510 at 2542.857 kb on - strand, within EX28DRAFT_2510 at 2542.857 kb on - strand, within EX28DRAFT_2510 at 2543.108 kb on - strand, within EX28DRAFT_2510 at 2543.174 kb on - strand at 2543.183 kb on + strand at 2543.184 kb on - strand at 2543.184 kb on - strand at 2543.184 kb on - strand at 2543.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,540,692 + EX28DRAFT_2508 0.79 +1.5 2,540,754 + EX28DRAFT_2508 0.81 -0.1 2,540,754 + EX28DRAFT_2508 0.81 +0.3 2,540,755 - EX28DRAFT_2508 0.81 +0.6 2,540,778 - EX28DRAFT_2508 0.82 +0.0 2,540,778 - EX28DRAFT_2508 0.82 +0.3 2,540,778 - EX28DRAFT_2508 0.82 -0.1 2,541,022 + EX28DRAFT_2508 0.89 +0.6 2,541,043 - -1.8 2,541,157 - +0.1 2,541,157 - +0.1 2,541,157 - -0.9 2,541,161 - -0.1 2,541,349 + +1.4 2,541,408 - +2.2 2,541,526 + -0.1 2,541,527 - +0.3 2,541,557 - EX28DRAFT_2509 0.10 -2.3 2,541,877 - EX28DRAFT_2509 0.40 -1.6 2,542,381 - EX28DRAFT_2509 0.87 -0.6 2,542,500 + -0.2 2,542,501 - +1.5 2,542,501 - +0.5 2,542,550 + -0.5 2,542,672 - EX28DRAFT_2510 0.12 +0.0 2,542,672 - EX28DRAFT_2510 0.12 -0.3 2,542,694 + EX28DRAFT_2510 0.16 +0.4 2,542,694 + EX28DRAFT_2510 0.16 -0.3 2,542,695 - EX28DRAFT_2510 0.16 -2.4 2,542,695 - EX28DRAFT_2510 0.16 -2.4 2,542,695 - EX28DRAFT_2510 0.16 -0.0 2,542,856 + EX28DRAFT_2510 0.42 +0.3 2,542,856 + EX28DRAFT_2510 0.42 -0.5 2,542,857 - EX28DRAFT_2510 0.42 +0.5 2,542,857 - EX28DRAFT_2510 0.42 -0.2 2,543,108 - EX28DRAFT_2510 0.83 +0.3 2,543,174 - -1.1 2,543,183 + +0.1 2,543,184 - -0.1 2,543,184 - +0.0 2,543,184 - +0.3 2,543,295 - -0.1
Or see this region's nucleotide sequence