Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2445 and EX28DRAFT_2446 are separated by 14 nucleotides EX28DRAFT_2446 and EX28DRAFT_2447 are separated by 34 nucleotides EX28DRAFT_2447 and EX28DRAFT_2448 are separated by 68 nucleotides
EX28DRAFT_2445: EX28DRAFT_2445 - P pilus assembly protein, porin PapC, at 2,475,184 to 2,477,745
_2445
EX28DRAFT_2446: EX28DRAFT_2446 - P pilus assembly protein, chaperone PapD, at 2,477,760 to 2,478,452
_2446
EX28DRAFT_2447: EX28DRAFT_2447 - P pilus assembly protein, pilin FimA, at 2,478,487 to 2,479,032
_2447
EX28DRAFT_2448: EX28DRAFT_2448 - P pilus assembly protein, pilin FimA, at 2,479,101 to 2,479,664
_2448
Position (kb)
2477
2478
2479 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2476.842 kb on - strand, within EX28DRAFT_2445 at 2476.850 kb on + strand, within EX28DRAFT_2445 at 2476.854 kb on + strand, within EX28DRAFT_2445 at 2476.855 kb on - strand, within EX28DRAFT_2445 at 2477.077 kb on - strand, within EX28DRAFT_2445 at 2477.136 kb on + strand, within EX28DRAFT_2445 at 2477.157 kb on - strand, within EX28DRAFT_2445 at 2477.183 kb on + strand, within EX28DRAFT_2445 at 2477.184 kb on - strand, within EX28DRAFT_2445 at 2477.186 kb on + strand, within EX28DRAFT_2445 at 2477.187 kb on - strand, within EX28DRAFT_2445 at 2477.187 kb on - strand, within EX28DRAFT_2445 at 2477.226 kb on - strand, within EX28DRAFT_2445 at 2477.464 kb on - strand, within EX28DRAFT_2445 at 2477.466 kb on + strand, within EX28DRAFT_2445 at 2477.467 kb on - strand, within EX28DRAFT_2445 at 2477.477 kb on - strand, within EX28DRAFT_2445 at 2477.929 kb on + strand, within EX28DRAFT_2446 at 2477.930 kb on - strand, within EX28DRAFT_2446 at 2477.932 kb on + strand, within EX28DRAFT_2446 at 2477.932 kb on + strand, within EX28DRAFT_2446 at 2477.933 kb on - strand, within EX28DRAFT_2446 at 2477.933 kb on - strand, within EX28DRAFT_2446 at 2478.128 kb on - strand, within EX28DRAFT_2446 at 2478.174 kb on - strand, within EX28DRAFT_2446 at 2478.174 kb on - strand, within EX28DRAFT_2446 at 2478.174 kb on - strand, within EX28DRAFT_2446 at 2478.177 kb on - strand, within EX28DRAFT_2446 at 2478.177 kb on - strand, within EX28DRAFT_2446 at 2478.280 kb on - strand, within EX28DRAFT_2446 at 2478.280 kb on - strand, within EX28DRAFT_2446 at 2478.280 kb on - strand, within EX28DRAFT_2446 at 2478.438 kb on - strand at 2478.468 kb on - strand at 2478.495 kb on - strand at 2478.505 kb on - strand at 2478.552 kb on + strand, within EX28DRAFT_2447 at 2478.808 kb on - strand, within EX28DRAFT_2447 at 2478.808 kb on - strand, within EX28DRAFT_2447 at 2478.808 kb on - strand, within EX28DRAFT_2447 at 2478.885 kb on + strand, within EX28DRAFT_2447 at 2478.886 kb on - strand, within EX28DRAFT_2447 at 2478.886 kb on - strand, within EX28DRAFT_2447 at 2479.013 kb on - strand at 2479.102 kb on + strand at 2479.102 kb on + strand at 2479.103 kb on - strand at 2479.103 kb on - strand at 2479.108 kb on + strand at 2479.109 kb on - strand at 2479.109 kb on - strand at 2479.109 kb on - strand at 2479.116 kb on + strand at 2479.175 kb on - strand, within EX28DRAFT_2448 at 2479.431 kb on - strand, within EX28DRAFT_2448 at 2479.433 kb on + strand, within EX28DRAFT_2448 at 2479.433 kb on + strand, within EX28DRAFT_2448 at 2479.434 kb on - strand, within EX28DRAFT_2448
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,476,842 - EX28DRAFT_2445 0.65 -0.7 2,476,850 + EX28DRAFT_2445 0.65 +0.9 2,476,854 + EX28DRAFT_2445 0.65 -1.1 2,476,855 - EX28DRAFT_2445 0.65 +0.1 2,477,077 - EX28DRAFT_2445 0.74 -1.7 2,477,136 + EX28DRAFT_2445 0.76 +0.1 2,477,157 - EX28DRAFT_2445 0.77 -0.2 2,477,183 + EX28DRAFT_2445 0.78 -0.3 2,477,184 - EX28DRAFT_2445 0.78 +1.0 2,477,186 + EX28DRAFT_2445 0.78 +0.7 2,477,187 - EX28DRAFT_2445 0.78 +1.0 2,477,187 - EX28DRAFT_2445 0.78 -1.6 2,477,226 - EX28DRAFT_2445 0.80 -0.5 2,477,464 - EX28DRAFT_2445 0.89 -2.0 2,477,466 + EX28DRAFT_2445 0.89 -0.6 2,477,467 - EX28DRAFT_2445 0.89 +0.3 2,477,477 - EX28DRAFT_2445 0.90 -0.1 2,477,929 + EX28DRAFT_2446 0.24 -1.0 2,477,930 - EX28DRAFT_2446 0.25 -1.8 2,477,932 + EX28DRAFT_2446 0.25 -0.0 2,477,932 + EX28DRAFT_2446 0.25 -0.8 2,477,933 - EX28DRAFT_2446 0.25 -3.0 2,477,933 - EX28DRAFT_2446 0.25 -0.3 2,478,128 - EX28DRAFT_2446 0.53 +0.1 2,478,174 - EX28DRAFT_2446 0.60 -0.3 2,478,174 - EX28DRAFT_2446 0.60 -2.3 2,478,174 - EX28DRAFT_2446 0.60 +0.6 2,478,177 - EX28DRAFT_2446 0.60 -0.5 2,478,177 - EX28DRAFT_2446 0.60 -2.5 2,478,280 - EX28DRAFT_2446 0.75 -2.2 2,478,280 - EX28DRAFT_2446 0.75 -1.3 2,478,280 - EX28DRAFT_2446 0.75 -0.3 2,478,438 - -0.1 2,478,468 - -0.4 2,478,495 - +0.0 2,478,505 - -0.3 2,478,552 + EX28DRAFT_2447 0.12 +1.6 2,478,808 - EX28DRAFT_2447 0.59 -1.5 2,478,808 - EX28DRAFT_2447 0.59 +1.0 2,478,808 - EX28DRAFT_2447 0.59 -0.5 2,478,885 + EX28DRAFT_2447 0.73 +0.3 2,478,886 - EX28DRAFT_2447 0.73 +1.0 2,478,886 - EX28DRAFT_2447 0.73 -0.3 2,479,013 - +0.3 2,479,102 + +0.2 2,479,102 + +0.0 2,479,103 - +1.2 2,479,103 - +0.7 2,479,108 + -0.5 2,479,109 - +0.6 2,479,109 - +0.2 2,479,109 - -0.2 2,479,116 + -0.3 2,479,175 - EX28DRAFT_2448 0.13 -0.1 2,479,431 - EX28DRAFT_2448 0.59 +0.9 2,479,433 + EX28DRAFT_2448 0.59 -0.6 2,479,433 + EX28DRAFT_2448 0.59 +0.3 2,479,434 - EX28DRAFT_2448 0.59 +1.0
Or see this region's nucleotide sequence