Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2127 and EX28DRAFT_2128 are separated by 240 nucleotides EX28DRAFT_2128 and EX28DRAFT_2129 are separated by 0 nucleotides
EX28DRAFT_2127: EX28DRAFT_2127 - D-alanyl-D-alanine carboxypeptidase, at 2,145,451 to 2,146,656
_2127
EX28DRAFT_2128: EX28DRAFT_2128 - Glutathione S-transferase, at 2,146,897 to 2,147,523
_2128
EX28DRAFT_2129: EX28DRAFT_2129 - Glucose/sorbosone dehydrogenases, at 2,147,524 to 2,148,642
_2129
Position (kb)
2146
2147
2148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2145.922 kb on - strand, within EX28DRAFT_2127 at 2146.015 kb on - strand, within EX28DRAFT_2127 at 2146.267 kb on - strand, within EX28DRAFT_2127 at 2146.270 kb on + strand, within EX28DRAFT_2127 at 2146.433 kb on + strand, within EX28DRAFT_2127 at 2146.503 kb on + strand, within EX28DRAFT_2127 at 2146.514 kb on + strand, within EX28DRAFT_2127 at 2146.515 kb on - strand, within EX28DRAFT_2127 at 2146.515 kb on - strand, within EX28DRAFT_2127 at 2146.656 kb on + strand at 2146.656 kb on + strand at 2146.657 kb on - strand at 2146.657 kb on - strand at 2146.720 kb on + strand at 2146.720 kb on + strand at 2146.783 kb on - strand at 2146.832 kb on + strand at 2146.845 kb on + strand at 2146.846 kb on - strand at 2146.892 kb on + strand at 2146.893 kb on - strand at 2146.893 kb on - strand at 2146.893 kb on - strand at 2146.896 kb on + strand at 2146.896 kb on + strand at 2146.897 kb on - strand at 2146.897 kb on - strand at 2146.897 kb on - strand at 2147.026 kb on + strand, within EX28DRAFT_2128 at 2147.026 kb on + strand, within EX28DRAFT_2128 at 2147.248 kb on - strand, within EX28DRAFT_2128 at 2147.344 kb on - strand, within EX28DRAFT_2128 at 2147.582 kb on + strand at 2147.583 kb on - strand at 2147.583 kb on - strand at 2147.583 kb on - strand at 2147.583 kb on - strand at 2147.584 kb on + strand at 2147.584 kb on + strand at 2147.584 kb on + strand at 2147.584 kb on + strand at 2147.584 kb on + strand at 2147.584 kb on + strand at 2147.585 kb on - strand at 2147.585 kb on - strand at 2147.585 kb on - strand at 2147.585 kb on - strand at 2147.585 kb on - strand at 2147.585 kb on - strand at 2147.620 kb on - strand at 2147.620 kb on - strand at 2147.861 kb on + strand, within EX28DRAFT_2129 at 2147.861 kb on + strand, within EX28DRAFT_2129 at 2147.862 kb on - strand, within EX28DRAFT_2129 at 2147.866 kb on - strand, within EX28DRAFT_2129 at 2147.894 kb on + strand, within EX28DRAFT_2129 at 2148.112 kb on - strand, within EX28DRAFT_2129 at 2148.141 kb on + strand, within EX28DRAFT_2129 at 2148.265 kb on - strand, within EX28DRAFT_2129 at 2148.504 kb on + strand, within EX28DRAFT_2129 at 2148.505 kb on - strand, within EX28DRAFT_2129
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,145,922 - EX28DRAFT_2127 0.39 -0.1 2,146,015 - EX28DRAFT_2127 0.47 +0.1 2,146,267 - EX28DRAFT_2127 0.68 -0.2 2,146,270 + EX28DRAFT_2127 0.68 +0.5 2,146,433 + EX28DRAFT_2127 0.81 +0.3 2,146,503 + EX28DRAFT_2127 0.87 +0.1 2,146,514 + EX28DRAFT_2127 0.88 +0.2 2,146,515 - EX28DRAFT_2127 0.88 +2.5 2,146,515 - EX28DRAFT_2127 0.88 -0.1 2,146,656 + +0.9 2,146,656 + -0.7 2,146,657 - +0.3 2,146,657 - -1.4 2,146,720 + -0.2 2,146,720 + -0.3 2,146,783 - +0.3 2,146,832 + +0.3 2,146,845 + -0.7 2,146,846 - -0.4 2,146,892 + +0.6 2,146,893 - +0.5 2,146,893 - +0.4 2,146,893 - +0.2 2,146,896 + +2.5 2,146,896 + -0.1 2,146,897 - -1.0 2,146,897 - -0.1 2,146,897 - -0.1 2,147,026 + EX28DRAFT_2128 0.21 -1.1 2,147,026 + EX28DRAFT_2128 0.21 -0.7 2,147,248 - EX28DRAFT_2128 0.56 -0.2 2,147,344 - EX28DRAFT_2128 0.71 -2.9 2,147,582 + -1.2 2,147,583 - +0.3 2,147,583 - +0.7 2,147,583 - -2.2 2,147,583 - -0.7 2,147,584 + +0.1 2,147,584 + -1.7 2,147,584 + -0.1 2,147,584 + -0.3 2,147,584 + -0.5 2,147,584 + -0.3 2,147,585 - +0.0 2,147,585 - +0.5 2,147,585 - -0.9 2,147,585 - -1.3 2,147,585 - -1.6 2,147,585 - -0.2 2,147,620 - -0.2 2,147,620 - +0.1 2,147,861 + EX28DRAFT_2129 0.30 -0.0 2,147,861 + EX28DRAFT_2129 0.30 -0.1 2,147,862 - EX28DRAFT_2129 0.30 +0.7 2,147,866 - EX28DRAFT_2129 0.31 +0.6 2,147,894 + EX28DRAFT_2129 0.33 +0.4 2,148,112 - EX28DRAFT_2129 0.53 +0.7 2,148,141 + EX28DRAFT_2129 0.55 +0.0 2,148,265 - EX28DRAFT_2129 0.66 -0.2 2,148,504 + EX28DRAFT_2129 0.88 +0.3 2,148,505 - EX28DRAFT_2129 0.88 +1.3
Or see this region's nucleotide sequence