Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2081 and EX28DRAFT_2082 overlap by 1 nucleotides EX28DRAFT_2082 and EX28DRAFT_2083 are separated by 17 nucleotides EX28DRAFT_2083 and EX28DRAFT_2084 are separated by 258 nucleotides
EX28DRAFT_2081: EX28DRAFT_2081 - Type II secretory pathway, component ExeA (predicted ATPase), at 2,106,935 to 2,108,509
_2081
EX28DRAFT_2082: EX28DRAFT_2082 - hypothetical protein, at 2,108,509 to 2,109,123
_2082
EX28DRAFT_2083: EX28DRAFT_2083 - hypothetical protein, at 2,109,141 to 2,109,764
_2083
EX28DRAFT_2084: EX28DRAFT_2084 - Negative regulator of beta-lactamase expression, at 2,110,023 to 2,110,853
_2084
Position (kb)
2108
2109
2110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2107.509 kb on - strand, within EX28DRAFT_2081 at 2107.509 kb on - strand, within EX28DRAFT_2081 at 2107.762 kb on - strand, within EX28DRAFT_2081 at 2107.891 kb on + strand, within EX28DRAFT_2081 at 2107.931 kb on + strand, within EX28DRAFT_2081 at 2107.931 kb on + strand, within EX28DRAFT_2081 at 2107.932 kb on - strand, within EX28DRAFT_2081 at 2107.933 kb on + strand, within EX28DRAFT_2081 at 2107.934 kb on - strand, within EX28DRAFT_2081 at 2108.006 kb on - strand, within EX28DRAFT_2081 at 2108.090 kb on + strand, within EX28DRAFT_2081 at 2108.090 kb on + strand, within EX28DRAFT_2081 at 2108.090 kb on + strand, within EX28DRAFT_2081 at 2108.090 kb on + strand, within EX28DRAFT_2081 at 2108.091 kb on - strand, within EX28DRAFT_2081 at 2108.091 kb on - strand, within EX28DRAFT_2081 at 2108.110 kb on - strand, within EX28DRAFT_2081 at 2108.174 kb on - strand, within EX28DRAFT_2081 at 2108.195 kb on + strand, within EX28DRAFT_2081 at 2108.408 kb on - strand at 2108.421 kb on + strand at 2108.452 kb on - strand at 2108.470 kb on + strand at 2108.470 kb on + strand at 2108.470 kb on + strand at 2108.470 kb on + strand at 2108.471 kb on - strand at 2108.471 kb on - strand at 2108.473 kb on - strand at 2108.473 kb on - strand at 2108.473 kb on - strand at 2108.520 kb on + strand at 2108.520 kb on + strand at 2108.605 kb on - strand, within EX28DRAFT_2082 at 2108.605 kb on - strand, within EX28DRAFT_2082 at 2108.749 kb on + strand, within EX28DRAFT_2082 at 2108.749 kb on + strand, within EX28DRAFT_2082 at 2108.750 kb on - strand, within EX28DRAFT_2082 at 2108.750 kb on - strand, within EX28DRAFT_2082 at 2108.750 kb on - strand, within EX28DRAFT_2082 at 2108.756 kb on - strand, within EX28DRAFT_2082 at 2108.892 kb on + strand, within EX28DRAFT_2082 at 2108.906 kb on + strand, within EX28DRAFT_2082 at 2109.416 kb on + strand, within EX28DRAFT_2083 at 2109.416 kb on + strand, within EX28DRAFT_2083 at 2109.512 kb on - strand, within EX28DRAFT_2083 at 2109.512 kb on - strand, within EX28DRAFT_2083 at 2109.515 kb on + strand, within EX28DRAFT_2083 at 2109.516 kb on - strand, within EX28DRAFT_2083 at 2109.554 kb on + strand, within EX28DRAFT_2083 at 2109.579 kb on - strand, within EX28DRAFT_2083 at 2109.635 kb on + strand, within EX28DRAFT_2083 at 2109.635 kb on + strand, within EX28DRAFT_2083 at 2109.659 kb on + strand, within EX28DRAFT_2083 at 2109.884 kb on - strand at 2109.901 kb on - strand at 2109.952 kb on + strand at 2109.996 kb on + strand at 2109.996 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,107,509 - EX28DRAFT_2081 0.36 -0.5 2,107,509 - EX28DRAFT_2081 0.36 +0.1 2,107,762 - EX28DRAFT_2081 0.53 -0.4 2,107,891 + EX28DRAFT_2081 0.61 +0.9 2,107,931 + EX28DRAFT_2081 0.63 -0.5 2,107,931 + EX28DRAFT_2081 0.63 -0.8 2,107,932 - EX28DRAFT_2081 0.63 -0.1 2,107,933 + EX28DRAFT_2081 0.63 +0.3 2,107,934 - EX28DRAFT_2081 0.63 -0.8 2,108,006 - EX28DRAFT_2081 0.68 -0.2 2,108,090 + EX28DRAFT_2081 0.73 -0.2 2,108,090 + EX28DRAFT_2081 0.73 -1.0 2,108,090 + EX28DRAFT_2081 0.73 +2.0 2,108,090 + EX28DRAFT_2081 0.73 +0.7 2,108,091 - EX28DRAFT_2081 0.73 -0.1 2,108,091 - EX28DRAFT_2081 0.73 -0.1 2,108,110 - EX28DRAFT_2081 0.75 +1.2 2,108,174 - EX28DRAFT_2081 0.79 +0.4 2,108,195 + EX28DRAFT_2081 0.80 -0.3 2,108,408 - +0.6 2,108,421 + -0.5 2,108,452 - -2.9 2,108,470 + -0.1 2,108,470 + -2.0 2,108,470 + +0.0 2,108,470 + +0.0 2,108,471 - +0.3 2,108,471 - -0.1 2,108,473 - -0.5 2,108,473 - +0.9 2,108,473 - +0.2 2,108,520 + -0.2 2,108,520 + +0.5 2,108,605 - EX28DRAFT_2082 0.16 +0.1 2,108,605 - EX28DRAFT_2082 0.16 +0.3 2,108,749 + EX28DRAFT_2082 0.39 -0.3 2,108,749 + EX28DRAFT_2082 0.39 -1.3 2,108,750 - EX28DRAFT_2082 0.39 -1.1 2,108,750 - EX28DRAFT_2082 0.39 -0.6 2,108,750 - EX28DRAFT_2082 0.39 -0.8 2,108,756 - EX28DRAFT_2082 0.40 -0.1 2,108,892 + EX28DRAFT_2082 0.62 -1.7 2,108,906 + EX28DRAFT_2082 0.65 -0.4 2,109,416 + EX28DRAFT_2083 0.44 -3.0 2,109,416 + EX28DRAFT_2083 0.44 +0.0 2,109,512 - EX28DRAFT_2083 0.59 -0.5 2,109,512 - EX28DRAFT_2083 0.59 -0.7 2,109,515 + EX28DRAFT_2083 0.60 -0.4 2,109,516 - EX28DRAFT_2083 0.60 +1.0 2,109,554 + EX28DRAFT_2083 0.66 -0.7 2,109,579 - EX28DRAFT_2083 0.70 +0.6 2,109,635 + EX28DRAFT_2083 0.79 -0.1 2,109,635 + EX28DRAFT_2083 0.79 +0.3 2,109,659 + EX28DRAFT_2083 0.83 +0.1 2,109,884 - +0.9 2,109,901 - -0.1 2,109,952 + +0.9 2,109,996 + -0.4 2,109,996 + -0.0
Or see this region's nucleotide sequence