Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2056 and EX28DRAFT_2057 are separated by 372 nucleotides EX28DRAFT_2057 and EX28DRAFT_2058 are separated by 178 nucleotides
EX28DRAFT_2056: EX28DRAFT_2056 - Predicted ATP-dependent endonuclease of the OLD family, at 2,077,666 to 2,079,324
_2056
EX28DRAFT_2057: EX28DRAFT_2057 - MIP family channel proteins, at 2,079,697 to 2,080,392
_2057
EX28DRAFT_2058: EX28DRAFT_2058 - Predicted membrane protein, at 2,080,571 to 2,081,470
_2058
Position (kb)
2079
2080
2081 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2079.099 kb on + strand, within EX28DRAFT_2056 at 2079.282 kb on + strand at 2079.282 kb on + strand at 2079.324 kb on + strand at 2079.360 kb on + strand at 2079.360 kb on + strand at 2079.370 kb on + strand at 2079.384 kb on + strand at 2079.384 kb on + strand at 2079.487 kb on + strand at 2079.488 kb on - strand at 2079.488 kb on - strand at 2079.488 kb on - strand at 2079.488 kb on - strand at 2079.510 kb on - strand at 2079.516 kb on + strand at 2079.516 kb on + strand at 2079.516 kb on + strand at 2079.517 kb on - strand at 2079.517 kb on - strand at 2079.559 kb on - strand at 2079.616 kb on - strand at 2079.652 kb on + strand at 2079.652 kb on + strand at 2079.652 kb on + strand at 2079.680 kb on - strand at 2079.729 kb on + strand at 2079.744 kb on - strand at 2079.836 kb on + strand, within EX28DRAFT_2057 at 2079.836 kb on + strand, within EX28DRAFT_2057 at 2079.837 kb on - strand, within EX28DRAFT_2057 at 2079.865 kb on - strand, within EX28DRAFT_2057 at 2079.899 kb on + strand, within EX28DRAFT_2057 at 2079.899 kb on + strand, within EX28DRAFT_2057 at 2079.905 kb on + strand, within EX28DRAFT_2057 at 2079.938 kb on + strand, within EX28DRAFT_2057 at 2079.938 kb on + strand, within EX28DRAFT_2057 at 2079.946 kb on + strand, within EX28DRAFT_2057 at 2079.946 kb on + strand, within EX28DRAFT_2057 at 2079.973 kb on - strand, within EX28DRAFT_2057 at 2079.975 kb on + strand, within EX28DRAFT_2057 at 2079.975 kb on + strand, within EX28DRAFT_2057 at 2079.975 kb on + strand, within EX28DRAFT_2057 at 2079.975 kb on + strand, within EX28DRAFT_2057 at 2079.994 kb on + strand, within EX28DRAFT_2057 at 2080.227 kb on + strand, within EX28DRAFT_2057 at 2080.526 kb on + strand at 2080.616 kb on + strand at 2080.654 kb on + strand at 2080.683 kb on - strand, within EX28DRAFT_2058 at 2080.687 kb on + strand, within EX28DRAFT_2058 at 2080.688 kb on - strand, within EX28DRAFT_2058 at 2080.784 kb on - strand, within EX28DRAFT_2058 at 2080.799 kb on - strand, within EX28DRAFT_2058 at 2080.902 kb on + strand, within EX28DRAFT_2058 at 2080.983 kb on - strand, within EX28DRAFT_2058 at 2080.984 kb on + strand, within EX28DRAFT_2058 at 2080.984 kb on + strand, within EX28DRAFT_2058 at 2080.984 kb on + strand, within EX28DRAFT_2058 at 2080.985 kb on - strand, within EX28DRAFT_2058 at 2080.985 kb on - strand, within EX28DRAFT_2058 at 2081.014 kb on + strand, within EX28DRAFT_2058 at 2081.029 kb on + strand, within EX28DRAFT_2058 at 2081.030 kb on - strand, within EX28DRAFT_2058 at 2081.131 kb on + strand, within EX28DRAFT_2058
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,079,099 + EX28DRAFT_2056 0.86 -0.5 2,079,282 + -1.9 2,079,282 + -0.9 2,079,324 + -0.2 2,079,360 + +0.5 2,079,360 + +0.3 2,079,370 + -0.7 2,079,384 + -0.6 2,079,384 + +1.1 2,079,487 + +0.0 2,079,488 - -0.3 2,079,488 - -1.3 2,079,488 - -0.2 2,079,488 - +0.9 2,079,510 - -0.1 2,079,516 + -0.0 2,079,516 + -0.9 2,079,516 + -0.8 2,079,517 - +1.9 2,079,517 - +0.0 2,079,559 - +0.0 2,079,616 - -0.1 2,079,652 + -0.0 2,079,652 + -0.5 2,079,652 + +0.0 2,079,680 - -0.5 2,079,729 + +0.3 2,079,744 - +0.3 2,079,836 + EX28DRAFT_2057 0.20 -0.5 2,079,836 + EX28DRAFT_2057 0.20 -0.4 2,079,837 - EX28DRAFT_2057 0.20 +0.2 2,079,865 - EX28DRAFT_2057 0.24 +0.4 2,079,899 + EX28DRAFT_2057 0.29 +0.0 2,079,899 + EX28DRAFT_2057 0.29 -0.4 2,079,905 + EX28DRAFT_2057 0.30 +0.0 2,079,938 + EX28DRAFT_2057 0.35 -0.4 2,079,938 + EX28DRAFT_2057 0.35 -0.2 2,079,946 + EX28DRAFT_2057 0.36 -0.5 2,079,946 + EX28DRAFT_2057 0.36 +2.5 2,079,973 - EX28DRAFT_2057 0.40 +0.1 2,079,975 + EX28DRAFT_2057 0.40 -1.4 2,079,975 + EX28DRAFT_2057 0.40 +0.1 2,079,975 + EX28DRAFT_2057 0.40 -1.0 2,079,975 + EX28DRAFT_2057 0.40 -0.6 2,079,994 + EX28DRAFT_2057 0.43 +0.4 2,080,227 + EX28DRAFT_2057 0.76 +0.2 2,080,526 + -0.6 2,080,616 + -0.4 2,080,654 + +0.2 2,080,683 - EX28DRAFT_2058 0.12 +1.1 2,080,687 + EX28DRAFT_2058 0.13 +0.1 2,080,688 - EX28DRAFT_2058 0.13 -0.3 2,080,784 - EX28DRAFT_2058 0.24 -0.9 2,080,799 - EX28DRAFT_2058 0.25 -1.7 2,080,902 + EX28DRAFT_2058 0.37 -0.3 2,080,983 - EX28DRAFT_2058 0.46 +0.0 2,080,984 + EX28DRAFT_2058 0.46 +0.4 2,080,984 + EX28DRAFT_2058 0.46 +0.2 2,080,984 + EX28DRAFT_2058 0.46 -0.3 2,080,985 - EX28DRAFT_2058 0.46 -0.0 2,080,985 - EX28DRAFT_2058 0.46 -1.9 2,081,014 + EX28DRAFT_2058 0.49 +0.5 2,081,029 + EX28DRAFT_2058 0.51 +1.3 2,081,030 - EX28DRAFT_2058 0.51 -0.4 2,081,131 + EX28DRAFT_2058 0.62 -0.6
Or see this region's nucleotide sequence