Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_2027

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_2025 and EX28DRAFT_2026 are separated by 111 nucleotidesEX28DRAFT_2026 and EX28DRAFT_2027 are separated by 185 nucleotidesEX28DRAFT_2027 and EX28DRAFT_2028 are separated by 69 nucleotides EX28DRAFT_2025: EX28DRAFT_2025 - ribosomal protein S1, at 2,036,456 to 2,038,129 _2025 EX28DRAFT_2026: EX28DRAFT_2026 - cytidylate kinase, at 2,038,241 to 2,038,924 _2026 EX28DRAFT_2027: EX28DRAFT_2027 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19), at 2,039,110 to 2,040,393 _2027 EX28DRAFT_2028: EX28DRAFT_2028 - phosphoserine aminotransferase apoenzyme (EC 2.6.1.52), at 2,040,463 to 2,041,515 _2028 Position (kb) 2039 2040 2041Strain fitness (log2 ratio) -3 -2 -1 0 1at 2038.937 kb on - strandat 2038.937 kb on - strandat 2038.941 kb on - strandat 2038.941 kb on - strandat 2038.974 kb on + strandat 2038.975 kb on - strandat 2038.975 kb on - strandat 2038.997 kb on + strandat 2039.015 kb on - strandat 2039.038 kb on - strandat 2039.103 kb on + strandat 2039.103 kb on + strandat 2039.104 kb on - strandat 2039.122 kb on - strandat 2039.122 kb on - strandat 2039.124 kb on + strandat 2039.125 kb on - strandat 2039.236 kb on - strandat 2039.301 kb on - strand, within EX28DRAFT_2027at 2039.390 kb on + strand, within EX28DRAFT_2027at 2039.390 kb on + strand, within EX28DRAFT_2027at 2039.390 kb on + strand, within EX28DRAFT_2027at 2039.614 kb on + strand, within EX28DRAFT_2027at 2039.795 kb on + strand, within EX28DRAFT_2027at 2039.835 kb on + strand, within EX28DRAFT_2027at 2039.922 kb on + strand, within EX28DRAFT_2027at 2040.376 kb on + strandat 2040.418 kb on + strandat 2040.419 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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2,038,937 - +0.2
2,038,937 - -0.6
2,038,941 - +0.0
2,038,941 - -0.3
2,038,974 + +0.9
2,038,975 - +0.3
2,038,975 - +0.3
2,038,997 + -0.0
2,039,015 - -0.8
2,039,038 - +0.5
2,039,103 + +0.4
2,039,103 + +0.0
2,039,104 - -1.0
2,039,122 - +0.4
2,039,122 - -0.1
2,039,124 + +0.1
2,039,125 - +0.5
2,039,236 - -1.6
2,039,301 - EX28DRAFT_2027 0.15 -2.0
2,039,390 + EX28DRAFT_2027 0.22 -2.3
2,039,390 + EX28DRAFT_2027 0.22 -1.7
2,039,390 + EX28DRAFT_2027 0.22 -2.1
2,039,614 + EX28DRAFT_2027 0.39 -2.2
2,039,795 + EX28DRAFT_2027 0.53 -2.1
2,039,835 + EX28DRAFT_2027 0.56 -2.5
2,039,922 + EX28DRAFT_2027 0.63 -3.6
2,040,376 + -2.5
2,040,418 + -1.6
2,040,419 - +0.1

Or see this region's nucleotide sequence