Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_2002

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_2001 and EX28DRAFT_2002 are separated by 9 nucleotidesEX28DRAFT_2002 and EX28DRAFT_2003 overlap by 4 nucleotidesEX28DRAFT_2003 and EX28DRAFT_2004 are separated by 51 nucleotides EX28DRAFT_2001: EX28DRAFT_2001 - alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,004,421 to 2,005,566 _2001 EX28DRAFT_2002: EX28DRAFT_2002 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 2,005,576 to 2,006,367 _2002 EX28DRAFT_2003: EX28DRAFT_2003 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 2,006,364 to 2,007,134 _2003 EX28DRAFT_2004: EX28DRAFT_2004 - aminopeptidase N, Escherichia coli type, at 2,007,186 to 2,009,798 _2004 Position (kb) 2005 2006 2007Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2004.796 kb on - strand, within EX28DRAFT_2001at 2004.805 kb on + strand, within EX28DRAFT_2001at 2004.882 kb on + strand, within EX28DRAFT_2001at 2004.882 kb on + strand, within EX28DRAFT_2001at 2004.883 kb on - strand, within EX28DRAFT_2001at 2004.908 kb on - strand, within EX28DRAFT_2001at 2004.908 kb on - strand, within EX28DRAFT_2001at 2004.944 kb on - strand, within EX28DRAFT_2001at 2005.000 kb on + strand, within EX28DRAFT_2001at 2005.401 kb on + strand, within EX28DRAFT_2001at 2005.565 kb on - strandat 2005.712 kb on + strand, within EX28DRAFT_2002at 2005.993 kb on + strand, within EX28DRAFT_2002at 2005.994 kb on - strand, within EX28DRAFT_2002at 2006.063 kb on + strand, within EX28DRAFT_2002at 2006.082 kb on + strand, within EX28DRAFT_2002at 2006.083 kb on - strand, within EX28DRAFT_2002at 2006.084 kb on + strand, within EX28DRAFT_2002at 2006.085 kb on - strand, within EX28DRAFT_2002at 2006.118 kb on + strand, within EX28DRAFT_2002at 2006.199 kb on - strand, within EX28DRAFT_2002at 2006.199 kb on - strand, within EX28DRAFT_2002at 2006.266 kb on + strand, within EX28DRAFT_2002at 2006.267 kb on - strand, within EX28DRAFT_2002at 2006.341 kb on + strandat 2006.341 kb on + strandat 2006.341 kb on + strandat 2006.341 kb on + strandat 2006.370 kb on - strandat 2006.370 kb on - strandat 2006.462 kb on + strand, within EX28DRAFT_2003at 2006.678 kb on + strand, within EX28DRAFT_2003at 2006.980 kb on + strand, within EX28DRAFT_2003at 2006.995 kb on + strand, within EX28DRAFT_2003at 2007.109 kb on + strandat 2007.119 kb on + strandat 2007.119 kb on + strandat 2007.119 kb on + strandat 2007.120 kb on - strandat 2007.120 kb on - strandat 2007.175 kb on + strandat 2007.176 kb on - strandat 2007.185 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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2,004,796 - EX28DRAFT_2001 0.33 -0.4
2,004,805 + EX28DRAFT_2001 0.34 +0.0
2,004,882 + EX28DRAFT_2001 0.40 -0.8
2,004,882 + EX28DRAFT_2001 0.40 -0.5
2,004,883 - EX28DRAFT_2001 0.40 +0.5
2,004,908 - EX28DRAFT_2001 0.42 +1.6
2,004,908 - EX28DRAFT_2001 0.42 -2.1
2,004,944 - EX28DRAFT_2001 0.46 +0.2
2,005,000 + EX28DRAFT_2001 0.51 +0.5
2,005,401 + EX28DRAFT_2001 0.86 -0.7
2,005,565 - +0.0
2,005,712 + EX28DRAFT_2002 0.17 +0.8
2,005,993 + EX28DRAFT_2002 0.53 +0.8
2,005,994 - EX28DRAFT_2002 0.53 +0.1
2,006,063 + EX28DRAFT_2002 0.61 +2.8
2,006,082 + EX28DRAFT_2002 0.64 +0.2
2,006,083 - EX28DRAFT_2002 0.64 -0.7
2,006,084 + EX28DRAFT_2002 0.64 +1.3
2,006,085 - EX28DRAFT_2002 0.64 +0.1
2,006,118 + EX28DRAFT_2002 0.68 -0.3
2,006,199 - EX28DRAFT_2002 0.79 +0.4
2,006,199 - EX28DRAFT_2002 0.79 +1.3
2,006,266 + EX28DRAFT_2002 0.87 +0.1
2,006,267 - EX28DRAFT_2002 0.87 -0.3
2,006,341 + +0.4
2,006,341 + +0.1
2,006,341 + +0.8
2,006,341 + -0.3
2,006,370 - -0.6
2,006,370 - -0.2
2,006,462 + EX28DRAFT_2003 0.13 +0.3
2,006,678 + EX28DRAFT_2003 0.41 +0.7
2,006,980 + EX28DRAFT_2003 0.80 -0.4
2,006,995 + EX28DRAFT_2003 0.82 +0.8
2,007,109 + -0.5
2,007,119 + +0.2
2,007,119 + +0.7
2,007,119 + +1.3
2,007,120 - +0.5
2,007,120 - +0.2
2,007,175 + -0.1
2,007,176 - +0.4
2,007,185 - -1.5

Or see this region's nucleotide sequence