Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1839 and EX28DRAFT_1840 are separated by 110 nucleotides EX28DRAFT_1840 and EX28DRAFT_1841 are separated by 16 nucleotides EX28DRAFT_1841 and EX28DRAFT_1842 are separated by 36 nucleotides EX28DRAFT_1842 and EX28DRAFT_1843 are separated by 35 nucleotides EX28DRAFT_1843 and EX28DRAFT_1844 are separated by 66 nucleotides
EX28DRAFT_1839: EX28DRAFT_1839 - phosphate:acyl-[acyl carrier protein] acyltransferase, at 1,855,681 to 1,856,685
_1839
EX28DRAFT_1840: EX28DRAFT_1840 - ribosomal protein L32, at 1,856,796 to 1,856,969
_1840
EX28DRAFT_1841: EX28DRAFT_1841 - Predicted metal-binding, possibly nucleic acid-binding protein, at 1,856,986 to 1,857,507
_1841
EX28DRAFT_1842: EX28DRAFT_1842 - RNA, at 1,857,544 to 1,857,654
_1842
EX28DRAFT_1843: EX28DRAFT_1843 - MAF protein, at 1,857,690 to 1,858,232
_1843
EX28DRAFT_1844: EX28DRAFT_1844 - pseudouridine synthase, RluA family, at 1,858,299 to 1,859,246
_1844
Position (kb)
1856
1857
1858 Strain fitness (log2 ratio)
-2
-1
0
1 at 1856.082 kb on + strand, within EX28DRAFT_1839 at 1856.083 kb on - strand, within EX28DRAFT_1839 at 1856.084 kb on + strand, within EX28DRAFT_1839 at 1856.084 kb on + strand, within EX28DRAFT_1839 at 1856.085 kb on - strand, within EX28DRAFT_1839 at 1856.247 kb on + strand, within EX28DRAFT_1839 at 1856.247 kb on + strand, within EX28DRAFT_1839 at 1856.248 kb on - strand, within EX28DRAFT_1839 at 1856.250 kb on + strand, within EX28DRAFT_1839 at 1856.353 kb on - strand, within EX28DRAFT_1839 at 1856.458 kb on - strand, within EX28DRAFT_1839 at 1856.472 kb on + strand, within EX28DRAFT_1839 at 1856.719 kb on - strand at 1857.430 kb on - strand, within EX28DRAFT_1841 at 1857.538 kb on - strand at 1857.579 kb on + strand, within EX28DRAFT_1842 at 1857.602 kb on - strand, within EX28DRAFT_1842 at 1857.629 kb on + strand, within EX28DRAFT_1842 at 1857.630 kb on - strand, within EX28DRAFT_1842 at 1857.632 kb on - strand, within EX28DRAFT_1842 at 1857.632 kb on - strand, within EX28DRAFT_1842 at 1857.845 kb on - strand, within EX28DRAFT_1843 at 1857.955 kb on - strand, within EX28DRAFT_1843 at 1857.972 kb on + strand, within EX28DRAFT_1843 at 1857.972 kb on + strand, within EX28DRAFT_1843 at 1857.973 kb on - strand, within EX28DRAFT_1843 at 1857.975 kb on - strand, within EX28DRAFT_1843 at 1858.021 kb on + strand, within EX28DRAFT_1843 at 1858.022 kb on - strand, within EX28DRAFT_1843 at 1858.060 kb on - strand, within EX28DRAFT_1843 at 1858.177 kb on + strand, within EX28DRAFT_1843 at 1858.275 kb on - strand at 1858.376 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,856,082 + EX28DRAFT_1839 0.40 -0.6 1,856,083 - EX28DRAFT_1839 0.40 -0.6 1,856,084 + EX28DRAFT_1839 0.40 -2.1 1,856,084 + EX28DRAFT_1839 0.40 -0.7 1,856,085 - EX28DRAFT_1839 0.40 -0.5 1,856,247 + EX28DRAFT_1839 0.56 +0.7 1,856,247 + EX28DRAFT_1839 0.56 +0.7 1,856,248 - EX28DRAFT_1839 0.56 -1.1 1,856,250 + EX28DRAFT_1839 0.57 -2.3 1,856,353 - EX28DRAFT_1839 0.67 -1.3 1,856,458 - EX28DRAFT_1839 0.77 -1.2 1,856,472 + EX28DRAFT_1839 0.79 -2.3 1,856,719 - -0.7 1,857,430 - EX28DRAFT_1841 0.85 -1.6 1,857,538 - +0.7 1,857,579 + EX28DRAFT_1842 0.32 +0.5 1,857,602 - EX28DRAFT_1842 0.52 +0.3 1,857,629 + EX28DRAFT_1842 0.77 -0.8 1,857,630 - EX28DRAFT_1842 0.77 +0.3 1,857,632 - EX28DRAFT_1842 0.79 +0.9 1,857,632 - EX28DRAFT_1842 0.79 -0.3 1,857,845 - EX28DRAFT_1843 0.29 +1.1 1,857,955 - EX28DRAFT_1843 0.49 -0.1 1,857,972 + EX28DRAFT_1843 0.52 +0.2 1,857,972 + EX28DRAFT_1843 0.52 +0.3 1,857,973 - EX28DRAFT_1843 0.52 +1.0 1,857,975 - EX28DRAFT_1843 0.52 -0.2 1,858,021 + EX28DRAFT_1843 0.61 -0.2 1,858,022 - EX28DRAFT_1843 0.61 -0.6 1,858,060 - EX28DRAFT_1843 0.68 +0.8 1,858,177 + EX28DRAFT_1843 0.90 -0.2 1,858,275 - -0.7 1,858,376 + +1.7
Or see this region's nucleotide sequence