Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1364 and EX28DRAFT_1365 are separated by 5 nucleotides EX28DRAFT_1365 and EX28DRAFT_1366 are separated by 95 nucleotides EX28DRAFT_1366 and EX28DRAFT_1367 are separated by 4 nucleotides EX28DRAFT_1367 and EX28DRAFT_1368 overlap by 4 nucleotides
EX28DRAFT_1364: EX28DRAFT_1364 - Methionine synthase II (cobalamin-independent), at 1,382,175 to 1,383,203
_1364
EX28DRAFT_1365: EX28DRAFT_1365 - Transcriptional regulator, at 1,383,209 to 1,384,141
_1365
EX28DRAFT_1366: EX28DRAFT_1366 - Uncharacterized protein conserved in bacteria, at 1,384,237 to 1,384,671
_1366
EX28DRAFT_1367: EX28DRAFT_1367 - Uncharacterized protein conserved in bacteria, at 1,384,676 to 1,385,134
_1367
EX28DRAFT_1368: EX28DRAFT_1368 - Protein of unknown function (DUF3833), at 1,385,131 to 1,385,664
_1368
Position (kb)
1383
1384
1385 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1382.393 kb on + strand, within EX28DRAFT_1364 at 1382.394 kb on - strand, within EX28DRAFT_1364 at 1382.610 kb on + strand, within EX28DRAFT_1364 at 1382.613 kb on - strand, within EX28DRAFT_1364 at 1382.613 kb on - strand, within EX28DRAFT_1364 at 1382.674 kb on + strand, within EX28DRAFT_1364 at 1382.921 kb on + strand, within EX28DRAFT_1364 at 1382.921 kb on + strand, within EX28DRAFT_1364 at 1382.982 kb on - strand, within EX28DRAFT_1364 at 1383.059 kb on + strand, within EX28DRAFT_1364 at 1383.249 kb on + strand at 1383.249 kb on + strand at 1383.250 kb on - strand at 1383.250 kb on - strand at 1383.300 kb on + strand at 1383.300 kb on + strand at 1383.301 kb on - strand at 1383.355 kb on + strand, within EX28DRAFT_1365 at 1383.615 kb on + strand, within EX28DRAFT_1365 at 1383.758 kb on + strand, within EX28DRAFT_1365 at 1383.978 kb on + strand, within EX28DRAFT_1365 at 1383.979 kb on - strand, within EX28DRAFT_1365 at 1384.204 kb on + strand at 1384.471 kb on + strand, within EX28DRAFT_1366 at 1384.617 kb on + strand, within EX28DRAFT_1366 at 1384.618 kb on - strand, within EX28DRAFT_1366 at 1384.636 kb on - strand at 1384.676 kb on - strand at 1384.686 kb on + strand at 1384.687 kb on - strand at 1384.843 kb on + strand, within EX28DRAFT_1367 at 1385.090 kb on + strand at 1385.091 kb on - strand at 1385.099 kb on + strand at 1385.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,382,393 + EX28DRAFT_1364 0.21 +1.1 1,382,394 - EX28DRAFT_1364 0.21 -0.9 1,382,610 + EX28DRAFT_1364 0.42 -0.9 1,382,613 - EX28DRAFT_1364 0.43 -0.2 1,382,613 - EX28DRAFT_1364 0.43 +0.1 1,382,674 + EX28DRAFT_1364 0.48 +1.6 1,382,921 + EX28DRAFT_1364 0.72 -0.4 1,382,921 + EX28DRAFT_1364 0.72 -0.5 1,382,982 - EX28DRAFT_1364 0.78 +0.5 1,383,059 + EX28DRAFT_1364 0.86 +0.5 1,383,249 + +1.4 1,383,249 + -0.9 1,383,250 - +0.1 1,383,250 - +0.2 1,383,300 + +0.3 1,383,300 + -0.3 1,383,301 - +3.2 1,383,355 + EX28DRAFT_1365 0.16 +0.4 1,383,615 + EX28DRAFT_1365 0.44 +0.1 1,383,758 + EX28DRAFT_1365 0.59 +0.3 1,383,978 + EX28DRAFT_1365 0.82 +0.6 1,383,979 - EX28DRAFT_1365 0.83 -0.5 1,384,204 + +0.2 1,384,471 + EX28DRAFT_1366 0.54 +0.2 1,384,617 + EX28DRAFT_1366 0.87 +0.0 1,384,618 - EX28DRAFT_1366 0.88 -0.1 1,384,636 - +0.1 1,384,676 - +1.4 1,384,686 + -0.7 1,384,687 - +0.1 1,384,843 + EX28DRAFT_1367 0.36 +0.7 1,385,090 + +0.4 1,385,091 - -0.0 1,385,099 + +1.4 1,385,100 - +1.7
Or see this region's nucleotide sequence