Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1358 and EX28DRAFT_1359 are separated by 90 nucleotides EX28DRAFT_1359 and EX28DRAFT_1360 are separated by 7 nucleotides EX28DRAFT_1360 and EX28DRAFT_1361 are separated by 311 nucleotides
EX28DRAFT_1358: EX28DRAFT_1358 - Acyl carrier protein phosphodiesterase, at 1,376,913 to 1,377,518
_1358
EX28DRAFT_1359: EX28DRAFT_1359 - Pseudouridylate synthases, 23S RNA-specific, at 1,377,609 to 1,378,295
_1359
EX28DRAFT_1360: EX28DRAFT_1360 - Uncharacterized protein conserved in bacteria, at 1,378,303 to 1,378,842
_1360
EX28DRAFT_1361: EX28DRAFT_1361 - hypothetical protein, at 1,379,154 to 1,380,269
_1361
Position (kb)
1378
1379 Strain fitness (log2 ratio)
-2
-1
0
1 at 1377.322 kb on + strand, within EX28DRAFT_1358 at 1377.621 kb on - strand at 1377.904 kb on - strand, within EX28DRAFT_1359 at 1377.949 kb on + strand, within EX28DRAFT_1359 at 1377.950 kb on - strand, within EX28DRAFT_1359 at 1377.950 kb on - strand, within EX28DRAFT_1359 at 1377.950 kb on - strand, within EX28DRAFT_1359 at 1378.053 kb on + strand, within EX28DRAFT_1359 at 1378.170 kb on + strand, within EX28DRAFT_1359 at 1378.242 kb on - strand at 1378.440 kb on + strand, within EX28DRAFT_1360 at 1378.569 kb on + strand, within EX28DRAFT_1360 at 1378.708 kb on - strand, within EX28DRAFT_1360 at 1378.877 kb on + strand at 1378.911 kb on + strand at 1379.035 kb on + strand at 1379.036 kb on - strand at 1379.036 kb on - strand at 1379.051 kb on + strand at 1379.326 kb on + strand, within EX28DRAFT_1361 at 1379.326 kb on + strand, within EX28DRAFT_1361 at 1379.326 kb on + strand, within EX28DRAFT_1361 at 1379.327 kb on - strand, within EX28DRAFT_1361 at 1379.347 kb on - strand, within EX28DRAFT_1361 at 1379.382 kb on + strand, within EX28DRAFT_1361 at 1379.382 kb on + strand, within EX28DRAFT_1361 at 1379.529 kb on + strand, within EX28DRAFT_1361 at 1379.530 kb on - strand, within EX28DRAFT_1361 at 1379.530 kb on - strand, within EX28DRAFT_1361 at 1379.532 kb on - strand, within EX28DRAFT_1361 at 1379.532 kb on - strand, within EX28DRAFT_1361 at 1379.532 kb on - strand, within EX28DRAFT_1361 at 1379.532 kb on - strand, within EX28DRAFT_1361 at 1379.700 kb on + strand, within EX28DRAFT_1361 at 1379.700 kb on + strand, within EX28DRAFT_1361 at 1379.700 kb on + strand, within EX28DRAFT_1361 at 1379.700 kb on + strand, within EX28DRAFT_1361 at 1379.794 kb on + strand, within EX28DRAFT_1361 at 1379.802 kb on - strand, within EX28DRAFT_1361
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,377,322 + EX28DRAFT_1358 0.67 -1.9 1,377,621 - -0.3 1,377,904 - EX28DRAFT_1359 0.43 -0.6 1,377,949 + EX28DRAFT_1359 0.49 +0.8 1,377,950 - EX28DRAFT_1359 0.50 -0.2 1,377,950 - EX28DRAFT_1359 0.50 -1.2 1,377,950 - EX28DRAFT_1359 0.50 -0.3 1,378,053 + EX28DRAFT_1359 0.65 +0.8 1,378,170 + EX28DRAFT_1359 0.82 -0.4 1,378,242 - +0.9 1,378,440 + EX28DRAFT_1360 0.25 -0.3 1,378,569 + EX28DRAFT_1360 0.49 -0.4 1,378,708 - EX28DRAFT_1360 0.75 -1.0 1,378,877 + -0.3 1,378,911 + +0.2 1,379,035 + -0.8 1,379,036 - +0.7 1,379,036 - +0.3 1,379,051 + -0.2 1,379,326 + EX28DRAFT_1361 0.15 +0.1 1,379,326 + EX28DRAFT_1361 0.15 -0.9 1,379,326 + EX28DRAFT_1361 0.15 -0.1 1,379,327 - EX28DRAFT_1361 0.16 +0.4 1,379,347 - EX28DRAFT_1361 0.17 -0.5 1,379,382 + EX28DRAFT_1361 0.20 +0.6 1,379,382 + EX28DRAFT_1361 0.20 -1.1 1,379,529 + EX28DRAFT_1361 0.34 -0.2 1,379,530 - EX28DRAFT_1361 0.34 -0.7 1,379,530 - EX28DRAFT_1361 0.34 -1.5 1,379,532 - EX28DRAFT_1361 0.34 +1.2 1,379,532 - EX28DRAFT_1361 0.34 -0.4 1,379,532 - EX28DRAFT_1361 0.34 +1.4 1,379,532 - EX28DRAFT_1361 0.34 -1.9 1,379,700 + EX28DRAFT_1361 0.49 -0.5 1,379,700 + EX28DRAFT_1361 0.49 -0.4 1,379,700 + EX28DRAFT_1361 0.49 +0.5 1,379,700 + EX28DRAFT_1361 0.49 -1.1 1,379,794 + EX28DRAFT_1361 0.57 +0.3 1,379,802 - EX28DRAFT_1361 0.58 -0.9
Or see this region's nucleotide sequence