Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1274 and EX28DRAFT_1275 are separated by 12 nucleotides EX28DRAFT_1275 and EX28DRAFT_1276 are separated by 57 nucleotides EX28DRAFT_1276 and EX28DRAFT_1277 are separated by 64 nucleotides EX28DRAFT_1277 and EX28DRAFT_1278 overlap by 4 nucleotides EX28DRAFT_1278 and EX28DRAFT_1279 are separated by 62 nucleotides
EX28DRAFT_1274: EX28DRAFT_1274 - hypothetical protein, at 1,291,120 to 1,294,545
_1274
EX28DRAFT_1275: EX28DRAFT_1275 - hypothetical protein, at 1,294,558 to 1,295,229
_1275
EX28DRAFT_1276: EX28DRAFT_1276 - Bacterial Ig-like domain (group 2), at 1,295,287 to 1,296,030
_1276
EX28DRAFT_1277: EX28DRAFT_1277 - hypothetical protein, at 1,296,095 to 1,296,478
_1277
EX28DRAFT_1278: EX28DRAFT_1278 - hypothetical protein, at 1,296,475 to 1,296,915
_1278
EX28DRAFT_1279: EX28DRAFT_1279 - hypothetical protein, at 1,296,978 to 1,297,265
_1279
Position (kb) 1295
1296
1297 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1294.601 kb on + strand at 1294.622 kb on + strand at 1294.656 kb on - strand, within EX28DRAFT_1275 at 1294.693 kb on + strand, within EX28DRAFT_1275 at 1294.748 kb on + strand, within EX28DRAFT_1275 at 1294.799 kb on - strand, within EX28DRAFT_1275 at 1294.799 kb on - strand, within EX28DRAFT_1275 at 1295.165 kb on + strand at 1295.165 kb on + strand at 1295.165 kb on + strand at 1295.230 kb on - strand at 1295.611 kb on + strand, within EX28DRAFT_1276 at 1295.661 kb on - strand, within EX28DRAFT_1276 at 1295.766 kb on - strand, within EX28DRAFT_1276 at 1295.874 kb on + strand, within EX28DRAFT_1276 at 1295.911 kb on - strand, within EX28DRAFT_1276 at 1296.025 kb on - strand at 1296.051 kb on - strand at 1296.051 kb on - strand at 1296.080 kb on - strand at 1296.135 kb on - strand, within EX28DRAFT_1277 at 1296.135 kb on - strand, within EX28DRAFT_1277 at 1296.228 kb on + strand, within EX28DRAFT_1277 at 1296.393 kb on + strand, within EX28DRAFT_1277 at 1296.393 kb on + strand, within EX28DRAFT_1277 at 1296.394 kb on - strand, within EX28DRAFT_1277 at 1296.394 kb on - strand, within EX28DRAFT_1277 at 1296.394 kb on - strand, within EX28DRAFT_1277 at 1296.513 kb on + strand at 1296.514 kb on - strand at 1296.514 kb on - strand at 1296.573 kb on - strand, within EX28DRAFT_1278 at 1296.683 kb on + strand, within EX28DRAFT_1278 at 1296.684 kb on - strand, within EX28DRAFT_1278 at 1296.684 kb on - strand, within EX28DRAFT_1278 at 1296.782 kb on + strand, within EX28DRAFT_1278 at 1296.783 kb on - strand, within EX28DRAFT_1278 at 1296.783 kb on - strand, within EX28DRAFT_1278 at 1296.785 kb on + strand, within EX28DRAFT_1278 at 1296.866 kb on + strand, within EX28DRAFT_1278 at 1296.866 kb on + strand, within EX28DRAFT_1278 at 1297.017 kb on - strand, within EX28DRAFT_1279
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,294,601 + -0.6 1,294,622 + -0.8 1,294,656 - EX28DRAFT_1275 0.15 +0.2 1,294,693 + EX28DRAFT_1275 0.20 +0.4 1,294,748 + EX28DRAFT_1275 0.28 -2.3 1,294,799 - EX28DRAFT_1275 0.36 -0.0 1,294,799 - EX28DRAFT_1275 0.36 +0.9 1,295,165 + -0.0 1,295,165 + +1.4 1,295,165 + +0.4 1,295,230 - -3.2 1,295,611 + EX28DRAFT_1276 0.44 -0.2 1,295,661 - EX28DRAFT_1276 0.50 -0.9 1,295,766 - EX28DRAFT_1276 0.64 +1.6 1,295,874 + EX28DRAFT_1276 0.79 -0.1 1,295,911 - EX28DRAFT_1276 0.84 +1.4 1,296,025 - +0.6 1,296,051 - -1.6 1,296,051 - -0.3 1,296,080 - -0.2 1,296,135 - EX28DRAFT_1277 0.10 +1.2 1,296,135 - EX28DRAFT_1277 0.10 +0.0 1,296,228 + EX28DRAFT_1277 0.35 -0.3 1,296,393 + EX28DRAFT_1277 0.78 +0.1 1,296,393 + EX28DRAFT_1277 0.78 -0.1 1,296,394 - EX28DRAFT_1277 0.78 +2.0 1,296,394 - EX28DRAFT_1277 0.78 -0.4 1,296,394 - EX28DRAFT_1277 0.78 -0.2 1,296,513 + -0.1 1,296,514 - -0.1 1,296,514 - +1.0 1,296,573 - EX28DRAFT_1278 0.22 +0.0 1,296,683 + EX28DRAFT_1278 0.47 -0.6 1,296,684 - EX28DRAFT_1278 0.47 +0.2 1,296,684 - EX28DRAFT_1278 0.47 -1.7 1,296,782 + EX28DRAFT_1278 0.70 -0.2 1,296,783 - EX28DRAFT_1278 0.70 -0.9 1,296,783 - EX28DRAFT_1278 0.70 +1.0 1,296,785 + EX28DRAFT_1278 0.70 -1.9 1,296,866 + EX28DRAFT_1278 0.89 -3.3 1,296,866 + EX28DRAFT_1278 0.89 +0.4 1,297,017 - EX28DRAFT_1279 0.14 +0.9
Or see this region's nucleotide sequence