Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1259 and EX28DRAFT_1260 are separated by 3 nucleotides EX28DRAFT_1260 and EX28DRAFT_1261 are separated by 13 nucleotides
EX28DRAFT_1259: EX28DRAFT_1259 - Uncharacterized conserved protein, at 1,277,280 to 1,277,948
_1259
EX28DRAFT_1260: EX28DRAFT_1260 - hypothetical protein, at 1,277,952 to 1,278,662
_1260
EX28DRAFT_1261: EX28DRAFT_1261 - Nucleotidyltransferase/DNA polymerase involved in DNA repair, at 1,278,676 to 1,279,944
_1261
Position (kb)
1277
1278
1279 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1276.965 kb on + strand at 1276.966 kb on - strand at 1277.030 kb on - strand at 1277.034 kb on - strand at 1277.052 kb on + strand at 1277.053 kb on - strand at 1277.053 kb on - strand at 1277.053 kb on - strand at 1277.169 kb on + strand at 1277.170 kb on - strand at 1277.188 kb on + strand at 1277.210 kb on - strand at 1277.210 kb on - strand at 1277.216 kb on + strand at 1277.235 kb on + strand at 1277.235 kb on + strand at 1277.236 kb on - strand at 1277.240 kb on - strand at 1277.240 kb on - strand at 1277.240 kb on - strand at 1277.249 kb on - strand at 1277.250 kb on + strand at 1277.250 kb on + strand at 1277.279 kb on + strand at 1277.279 kb on + strand at 1277.279 kb on + strand at 1277.320 kb on - strand at 1277.328 kb on + strand at 1277.328 kb on + strand at 1277.361 kb on + strand, within EX28DRAFT_1259 at 1277.466 kb on + strand, within EX28DRAFT_1259 at 1277.467 kb on - strand, within EX28DRAFT_1259 at 1277.486 kb on + strand, within EX28DRAFT_1259 at 1277.487 kb on - strand, within EX28DRAFT_1259 at 1277.907 kb on - strand at 1277.947 kb on - strand at 1278.466 kb on - strand, within EX28DRAFT_1260 at 1278.510 kb on - strand, within EX28DRAFT_1260 at 1278.510 kb on - strand, within EX28DRAFT_1260 at 1278.539 kb on + strand, within EX28DRAFT_1260 at 1278.688 kb on + strand at 1278.776 kb on + strand at 1278.776 kb on + strand at 1278.871 kb on + strand, within EX28DRAFT_1261 at 1278.979 kb on - strand, within EX28DRAFT_1261 at 1278.979 kb on - strand, within EX28DRAFT_1261 at 1279.146 kb on + strand, within EX28DRAFT_1261 at 1279.147 kb on - strand, within EX28DRAFT_1261 at 1279.147 kb on - strand, within EX28DRAFT_1261 at 1279.283 kb on - strand, within EX28DRAFT_1261 at 1279.397 kb on + strand, within EX28DRAFT_1261 at 1279.397 kb on + strand, within EX28DRAFT_1261 at 1279.398 kb on - strand, within EX28DRAFT_1261 at 1279.398 kb on - strand, within EX28DRAFT_1261 at 1279.398 kb on - strand, within EX28DRAFT_1261 at 1279.398 kb on - strand, within EX28DRAFT_1261 at 1279.440 kb on + strand, within EX28DRAFT_1261 at 1279.441 kb on - strand, within EX28DRAFT_1261 at 1279.546 kb on - strand, within EX28DRAFT_1261 at 1279.596 kb on + strand, within EX28DRAFT_1261 at 1279.647 kb on + strand, within EX28DRAFT_1261 at 1279.648 kb on - strand, within EX28DRAFT_1261 at 1279.652 kb on + strand, within EX28DRAFT_1261 at 1279.652 kb on + strand, within EX28DRAFT_1261
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,276,965 + +0.7 1,276,966 - -0.2 1,277,030 - +0.8 1,277,034 - -0.2 1,277,052 + +0.3 1,277,053 - +0.9 1,277,053 - +0.4 1,277,053 - +0.3 1,277,169 + -0.0 1,277,170 - +0.5 1,277,188 + -0.2 1,277,210 - +0.2 1,277,210 - -0.7 1,277,216 + +0.1 1,277,235 + +0.5 1,277,235 + -0.3 1,277,236 - +0.5 1,277,240 - -0.0 1,277,240 - -0.3 1,277,240 - -0.2 1,277,249 - +0.4 1,277,250 + -0.0 1,277,250 + -0.1 1,277,279 + -0.7 1,277,279 + -0.4 1,277,279 + +0.2 1,277,320 - -0.7 1,277,328 + -0.1 1,277,328 + -1.6 1,277,361 + EX28DRAFT_1259 0.12 -0.0 1,277,466 + EX28DRAFT_1259 0.28 -0.7 1,277,467 - EX28DRAFT_1259 0.28 +0.0 1,277,486 + EX28DRAFT_1259 0.31 +0.8 1,277,487 - EX28DRAFT_1259 0.31 +0.1 1,277,907 - -0.9 1,277,947 - -1.2 1,278,466 - EX28DRAFT_1260 0.72 -1.2 1,278,510 - EX28DRAFT_1260 0.78 -0.6 1,278,510 - EX28DRAFT_1260 0.78 -0.3 1,278,539 + EX28DRAFT_1260 0.83 -2.2 1,278,688 + +0.2 1,278,776 + -0.9 1,278,776 + -1.2 1,278,871 + EX28DRAFT_1261 0.15 -0.8 1,278,979 - EX28DRAFT_1261 0.24 +0.1 1,278,979 - EX28DRAFT_1261 0.24 -0.3 1,279,146 + EX28DRAFT_1261 0.37 +0.4 1,279,147 - EX28DRAFT_1261 0.37 -0.8 1,279,147 - EX28DRAFT_1261 0.37 +0.4 1,279,283 - EX28DRAFT_1261 0.48 +0.5 1,279,397 + EX28DRAFT_1261 0.57 +0.6 1,279,397 + EX28DRAFT_1261 0.57 +0.1 1,279,398 - EX28DRAFT_1261 0.57 +0.3 1,279,398 - EX28DRAFT_1261 0.57 +0.4 1,279,398 - EX28DRAFT_1261 0.57 -3.1 1,279,398 - EX28DRAFT_1261 0.57 +0.3 1,279,440 + EX28DRAFT_1261 0.60 +0.1 1,279,441 - EX28DRAFT_1261 0.60 -0.2 1,279,546 - EX28DRAFT_1261 0.69 -0.1 1,279,596 + EX28DRAFT_1261 0.72 -0.2 1,279,647 + EX28DRAFT_1261 0.77 -0.1 1,279,648 - EX28DRAFT_1261 0.77 -0.4 1,279,652 + EX28DRAFT_1261 0.77 -0.0 1,279,652 + EX28DRAFT_1261 0.77 -0.6
Or see this region's nucleotide sequence