Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1036 and EX28DRAFT_1037 are separated by 95 nucleotides EX28DRAFT_1037 and EX28DRAFT_1038 are separated by 3 nucleotides EX28DRAFT_1038 and EX28DRAFT_1039 overlap by 4 nucleotides EX28DRAFT_1039 and EX28DRAFT_1040 are separated by 148 nucleotides EX28DRAFT_1040 and EX28DRAFT_1041 are separated by 65 nucleotides
EX28DRAFT_1036: EX28DRAFT_1036 - ABC-type dipeptide transport system, periplasmic component, at 1,046,673 to 1,048,271
_1036
EX28DRAFT_1037: EX28DRAFT_1037 - Protein of unknown function (DUF2526), at 1,048,367 to 1,048,600
_1037
EX28DRAFT_1038: EX28DRAFT_1038 - Uncharacterized protein conserved in bacteria, at 1,048,604 to 1,049,053
_1038
EX28DRAFT_1039: EX28DRAFT_1039 - Sortase and related acyltransferases, at 1,049,050 to 1,049,568
_1039
EX28DRAFT_1040: EX28DRAFT_1040 - Cupin domain/Helix-turn-helix, at 1,049,717 to 1,050,292
_1040
EX28DRAFT_1041: EX28DRAFT_1041 - Putative NADP-dependent oxidoreductases, at 1,050,358 to 1,051,398
_1041
Position (kb) 1049
1050 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1048.194 kb on + strand at 1048.194 kb on + strand at 1048.194 kb on + strand at 1048.195 kb on - strand at 1048.265 kb on + strand at 1048.265 kb on + strand at 1048.265 kb on + strand at 1048.266 kb on - strand at 1048.269 kb on + strand at 1048.269 kb on + strand at 1048.269 kb on + strand at 1048.270 kb on - strand at 1048.270 kb on - strand at 1048.272 kb on + strand at 1048.272 kb on + strand at 1048.306 kb on - strand at 1048.387 kb on + strand at 1048.490 kb on + strand, within EX28DRAFT_1037 at 1048.534 kb on + strand, within EX28DRAFT_1037 at 1048.910 kb on + strand, within EX28DRAFT_1038 at 1048.911 kb on - strand, within EX28DRAFT_1038 at 1048.911 kb on - strand, within EX28DRAFT_1038 at 1048.985 kb on + strand, within EX28DRAFT_1038 at 1049.009 kb on + strand at 1049.097 kb on + strand at 1049.496 kb on + strand, within EX28DRAFT_1039 at 1049.705 kb on + strand at 1049.705 kb on + strand at 1049.705 kb on + strand at 1049.706 kb on - strand at 1049.708 kb on + strand at 1049.708 kb on + strand at 1049.708 kb on + strand at 1049.708 kb on + strand at 1050.005 kb on + strand, within EX28DRAFT_1040 at 1050.005 kb on + strand, within EX28DRAFT_1040 at 1050.104 kb on + strand, within EX28DRAFT_1040 at 1050.124 kb on + strand, within EX28DRAFT_1040 at 1050.125 kb on - strand, within EX28DRAFT_1040 at 1050.257 kb on + strand at 1050.258 kb on - strand at 1050.258 kb on - strand at 1050.267 kb on + strand at 1050.268 kb on - strand at 1050.290 kb on + strand at 1050.291 kb on - strand at 1050.297 kb on - strand at 1050.514 kb on + strand, within EX28DRAFT_1041 at 1050.514 kb on + strand, within EX28DRAFT_1041 at 1050.515 kb on - strand, within EX28DRAFT_1041 at 1050.547 kb on + strand, within EX28DRAFT_1041 at 1050.547 kb on + strand, within EX28DRAFT_1041 at 1050.548 kb on - strand, within EX28DRAFT_1041 at 1050.548 kb on - strand, within EX28DRAFT_1041
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,048,194 + +2.5 1,048,194 + -0.1 1,048,194 + -0.4 1,048,195 - +0.9 1,048,265 + +0.3 1,048,265 + -0.3 1,048,265 + -1.2 1,048,266 - -0.4 1,048,269 + +0.8 1,048,269 + -0.4 1,048,269 + +0.0 1,048,270 - -0.2 1,048,270 - -1.1 1,048,272 + -0.9 1,048,272 + -0.1 1,048,306 - -0.5 1,048,387 + -0.7 1,048,490 + EX28DRAFT_1037 0.53 +0.2 1,048,534 + EX28DRAFT_1037 0.71 +0.5 1,048,910 + EX28DRAFT_1038 0.68 +3.1 1,048,911 - EX28DRAFT_1038 0.68 +1.7 1,048,911 - EX28DRAFT_1038 0.68 +0.5 1,048,985 + EX28DRAFT_1038 0.85 -0.4 1,049,009 + -0.6 1,049,097 + -0.6 1,049,496 + EX28DRAFT_1039 0.86 -0.9 1,049,705 + -1.1 1,049,705 + -0.5 1,049,705 + -0.3 1,049,706 - -2.0 1,049,708 + +0.4 1,049,708 + +0.9 1,049,708 + -0.6 1,049,708 + -0.1 1,050,005 + EX28DRAFT_1040 0.50 -0.6 1,050,005 + EX28DRAFT_1040 0.50 +0.2 1,050,104 + EX28DRAFT_1040 0.67 +0.3 1,050,124 + EX28DRAFT_1040 0.71 -0.5 1,050,125 - EX28DRAFT_1040 0.71 -0.1 1,050,257 + +3.5 1,050,258 - -0.5 1,050,258 - -0.5 1,050,267 + -0.9 1,050,268 - +0.2 1,050,290 + -0.2 1,050,291 - +0.3 1,050,297 - -1.2 1,050,514 + EX28DRAFT_1041 0.15 -1.5 1,050,514 + EX28DRAFT_1041 0.15 +0.3 1,050,515 - EX28DRAFT_1041 0.15 -0.9 1,050,547 + EX28DRAFT_1041 0.18 -0.3 1,050,547 + EX28DRAFT_1041 0.18 -0.5 1,050,548 - EX28DRAFT_1041 0.18 -0.1 1,050,548 - EX28DRAFT_1041 0.18 +0.2
Or see this region's nucleotide sequence