Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0934 and EX28DRAFT_0935 are separated by 3 nucleotides EX28DRAFT_0935 and EX28DRAFT_0936 overlap by 1 nucleotides
EX28DRAFT_0934: EX28DRAFT_0934 - indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 932,695 to 934,053
_0934
EX28DRAFT_0935: EX28DRAFT_0935 - glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase, at 934,057 to 935,652
_0935
EX28DRAFT_0936: EX28DRAFT_0936 - anthranilate synthase, component I (EC 4.1.3.27), at 935,652 to 937,214
_0936
Position (kb)
934
935
936 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 933.108 kb on + strand, within EX28DRAFT_0934 at 933.108 kb on + strand, within EX28DRAFT_0934 at 933.108 kb on + strand, within EX28DRAFT_0934 at 933.134 kb on + strand, within EX28DRAFT_0934 at 933.134 kb on + strand, within EX28DRAFT_0934 at 933.134 kb on + strand, within EX28DRAFT_0934 at 933.135 kb on - strand, within EX28DRAFT_0934 at 933.135 kb on - strand, within EX28DRAFT_0934 at 933.163 kb on - strand, within EX28DRAFT_0934 at 933.163 kb on - strand, within EX28DRAFT_0934 at 933.377 kb on - strand, within EX28DRAFT_0934 at 933.377 kb on - strand, within EX28DRAFT_0934 at 933.669 kb on - strand, within EX28DRAFT_0934 at 933.669 kb on - strand, within EX28DRAFT_0934 at 933.702 kb on + strand, within EX28DRAFT_0934 at 933.706 kb on - strand, within EX28DRAFT_0934 at 933.824 kb on + strand, within EX28DRAFT_0934 at 933.935 kb on + strand at 934.111 kb on + strand at 934.112 kb on - strand at 934.112 kb on - strand at 934.112 kb on - strand at 934.228 kb on + strand, within EX28DRAFT_0935 at 934.290 kb on - strand, within EX28DRAFT_0935 at 934.290 kb on - strand, within EX28DRAFT_0935 at 934.326 kb on - strand, within EX28DRAFT_0935 at 934.326 kb on - strand, within EX28DRAFT_0935 at 934.596 kb on - strand, within EX28DRAFT_0935 at 934.662 kb on + strand, within EX28DRAFT_0935 at 934.674 kb on + strand, within EX28DRAFT_0935 at 934.789 kb on - strand, within EX28DRAFT_0935 at 934.801 kb on - strand, within EX28DRAFT_0935 at 934.828 kb on - strand, within EX28DRAFT_0935 at 935.235 kb on + strand, within EX28DRAFT_0935 at 935.373 kb on - strand, within EX28DRAFT_0935 at 935.565 kb on + strand at 935.565 kb on + strand at 935.609 kb on + strand at 935.610 kb on - strand at 935.626 kb on - strand at 935.681 kb on + strand at 936.038 kb on - strand, within EX28DRAFT_0936 at 936.340 kb on + strand, within EX28DRAFT_0936 at 936.341 kb on - strand, within EX28DRAFT_0936 at 936.374 kb on - strand, within EX28DRAFT_0936
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 933,108 + EX28DRAFT_0934 0.30 -4.1 933,108 + EX28DRAFT_0934 0.30 -3.3 933,108 + EX28DRAFT_0934 0.30 +0.6 933,134 + EX28DRAFT_0934 0.32 -1.6 933,134 + EX28DRAFT_0934 0.32 -1.0 933,134 + EX28DRAFT_0934 0.32 -2.9 933,135 - EX28DRAFT_0934 0.32 -3.6 933,135 - EX28DRAFT_0934 0.32 -2.6 933,163 - EX28DRAFT_0934 0.34 -3.2 933,163 - EX28DRAFT_0934 0.34 -2.7 933,377 - EX28DRAFT_0934 0.50 -2.3 933,377 - EX28DRAFT_0934 0.50 -0.4 933,669 - EX28DRAFT_0934 0.72 -1.0 933,669 - EX28DRAFT_0934 0.72 -3.1 933,702 + EX28DRAFT_0934 0.74 -1.0 933,706 - EX28DRAFT_0934 0.74 -2.4 933,824 + EX28DRAFT_0934 0.83 -1.7 933,935 + +0.6 934,111 + -0.4 934,112 - -0.4 934,112 - -3.4 934,112 - -3.2 934,228 + EX28DRAFT_0935 0.11 -1.2 934,290 - EX28DRAFT_0935 0.15 -2.6 934,290 - EX28DRAFT_0935 0.15 -1.6 934,326 - EX28DRAFT_0935 0.17 -1.7 934,326 - EX28DRAFT_0935 0.17 -0.5 934,596 - EX28DRAFT_0935 0.34 -2.7 934,662 + EX28DRAFT_0935 0.38 -4.3 934,674 + EX28DRAFT_0935 0.39 +0.2 934,789 - EX28DRAFT_0935 0.46 -1.2 934,801 - EX28DRAFT_0935 0.47 -2.2 934,828 - EX28DRAFT_0935 0.48 -2.7 935,235 + EX28DRAFT_0935 0.74 -1.0 935,373 - EX28DRAFT_0935 0.82 -4.9 935,565 + -2.7 935,565 + -3.1 935,609 + -2.2 935,610 - -3.3 935,626 - -3.7 935,681 + -3.0 936,038 - EX28DRAFT_0936 0.25 -0.7 936,340 + EX28DRAFT_0936 0.44 -2.3 936,341 - EX28DRAFT_0936 0.44 -3.4 936,374 - EX28DRAFT_0936 0.46 -1.4
Or see this region's nucleotide sequence