Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0653 and EX28DRAFT_0654 are separated by 14 nucleotides EX28DRAFT_0654 and EX28DRAFT_0655 are separated by 176 nucleotides EX28DRAFT_0655 and EX28DRAFT_0656 overlap by 26 nucleotides
EX28DRAFT_0653: EX28DRAFT_0653 - ABC-type sugar transport system, ATPase component, at 647,019 to 648,533
_0653
EX28DRAFT_0654: EX28DRAFT_0654 - Ribose/xylose/arabinose/galactoside ABC-type transport systems, permease components, at 648,548 to 649,534
_0654
EX28DRAFT_0655: EX28DRAFT_0655 - trehalose-phosphatase, at 649,711 to 650,514
_0655
EX28DRAFT_0656: EX28DRAFT_0656 - alpha,alpha-trehalose-phosphate synthase [UDP-forming], at 650,489 to 651,913
_0656
Position (kb) 648
649
650 Strain fitness (log2 ratio) -2
-1
0
1
2 at 647.635 kb on + strand, within EX28DRAFT_0653 at 647.636 kb on - strand, within EX28DRAFT_0653 at 647.636 kb on - strand, within EX28DRAFT_0653 at 647.763 kb on + strand, within EX28DRAFT_0653 at 647.764 kb on - strand, within EX28DRAFT_0653 at 647.960 kb on + strand, within EX28DRAFT_0653 at 648.038 kb on + strand, within EX28DRAFT_0653 at 648.085 kb on + strand, within EX28DRAFT_0653 at 648.087 kb on - strand, within EX28DRAFT_0653 at 648.183 kb on - strand, within EX28DRAFT_0653 at 648.247 kb on + strand, within EX28DRAFT_0653 at 648.269 kb on + strand, within EX28DRAFT_0653 at 648.269 kb on + strand, within EX28DRAFT_0653 at 648.269 kb on + strand, within EX28DRAFT_0653 at 648.333 kb on + strand, within EX28DRAFT_0653 at 648.333 kb on + strand, within EX28DRAFT_0653 at 648.334 kb on - strand, within EX28DRAFT_0653 at 648.335 kb on + strand, within EX28DRAFT_0653 at 648.335 kb on + strand, within EX28DRAFT_0653 at 648.345 kb on + strand, within EX28DRAFT_0653 at 648.345 kb on + strand, within EX28DRAFT_0653 at 648.345 kb on + strand, within EX28DRAFT_0653 at 648.346 kb on - strand, within EX28DRAFT_0653 at 648.535 kb on + strand at 648.535 kb on + strand at 648.536 kb on - strand at 648.544 kb on - strand at 648.544 kb on - strand at 648.560 kb on - strand at 648.562 kb on + strand at 648.607 kb on + strand at 648.607 kb on + strand at 648.694 kb on + strand, within EX28DRAFT_0654 at 648.697 kb on + strand, within EX28DRAFT_0654 at 648.698 kb on - strand, within EX28DRAFT_0654 at 648.698 kb on - strand, within EX28DRAFT_0654 at 648.986 kb on + strand, within EX28DRAFT_0654 at 648.988 kb on + strand, within EX28DRAFT_0654 at 648.988 kb on + strand, within EX28DRAFT_0654 at 648.989 kb on - strand, within EX28DRAFT_0654 at 648.989 kb on - strand, within EX28DRAFT_0654 at 649.145 kb on - strand, within EX28DRAFT_0654 at 649.213 kb on + strand, within EX28DRAFT_0654 at 649.258 kb on + strand, within EX28DRAFT_0654 at 649.259 kb on - strand, within EX28DRAFT_0654 at 649.259 kb on - strand, within EX28DRAFT_0654 at 649.379 kb on + strand, within EX28DRAFT_0654 at 649.380 kb on - strand, within EX28DRAFT_0654 at 649.380 kb on - strand, within EX28DRAFT_0654 at 649.457 kb on + strand at 649.565 kb on - strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.647 kb on - strand at 649.649 kb on - strand at 649.649 kb on - strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.753 kb on + strand at 649.754 kb on - strand at 649.754 kb on - strand at 649.819 kb on - strand, within EX28DRAFT_0655 at 649.992 kb on + strand, within EX28DRAFT_0655 at 650.213 kb on + strand, within EX28DRAFT_0655 at 650.214 kb on - strand, within EX28DRAFT_0655 at 650.232 kb on + strand, within EX28DRAFT_0655 at 650.430 kb on - strand, within EX28DRAFT_0655 at 650.462 kb on + strand at 650.463 kb on - strand at 650.499 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 647,635 + EX28DRAFT_0653 0.41 +0.3 647,636 - EX28DRAFT_0653 0.41 -0.2 647,636 - EX28DRAFT_0653 0.41 -1.0 647,763 + EX28DRAFT_0653 0.49 -0.0 647,764 - EX28DRAFT_0653 0.49 -0.0 647,960 + EX28DRAFT_0653 0.62 -0.4 648,038 + EX28DRAFT_0653 0.67 +1.3 648,085 + EX28DRAFT_0653 0.70 +0.3 648,087 - EX28DRAFT_0653 0.70 +0.4 648,183 - EX28DRAFT_0653 0.77 +0.2 648,247 + EX28DRAFT_0653 0.81 +0.2 648,269 + EX28DRAFT_0653 0.83 -0.4 648,269 + EX28DRAFT_0653 0.83 -0.1 648,269 + EX28DRAFT_0653 0.83 +0.3 648,333 + EX28DRAFT_0653 0.87 -0.2 648,333 + EX28DRAFT_0653 0.87 -0.3 648,334 - EX28DRAFT_0653 0.87 -2.3 648,335 + EX28DRAFT_0653 0.87 -0.5 648,335 + EX28DRAFT_0653 0.87 +0.7 648,345 + EX28DRAFT_0653 0.88 +0.5 648,345 + EX28DRAFT_0653 0.88 -1.6 648,345 + EX28DRAFT_0653 0.88 -0.1 648,346 - EX28DRAFT_0653 0.88 -0.4 648,535 + +0.3 648,535 + -0.6 648,536 - -0.5 648,544 - -1.0 648,544 - -0.5 648,560 - -0.5 648,562 + -1.2 648,607 + +0.3 648,607 + -0.5 648,694 + EX28DRAFT_0654 0.15 -0.5 648,697 + EX28DRAFT_0654 0.15 +0.5 648,698 - EX28DRAFT_0654 0.15 +0.3 648,698 - EX28DRAFT_0654 0.15 -0.2 648,986 + EX28DRAFT_0654 0.44 +0.9 648,988 + EX28DRAFT_0654 0.45 +0.7 648,988 + EX28DRAFT_0654 0.45 -0.8 648,989 - EX28DRAFT_0654 0.45 -1.5 648,989 - EX28DRAFT_0654 0.45 -0.4 649,145 - EX28DRAFT_0654 0.60 +1.5 649,213 + EX28DRAFT_0654 0.67 -1.0 649,258 + EX28DRAFT_0654 0.72 +0.9 649,259 - EX28DRAFT_0654 0.72 -0.2 649,259 - EX28DRAFT_0654 0.72 -0.7 649,379 + EX28DRAFT_0654 0.84 -0.4 649,380 - EX28DRAFT_0654 0.84 +2.3 649,380 - EX28DRAFT_0654 0.84 +0.0 649,457 + -0.4 649,565 - +0.3 649,572 + +0.3 649,572 + -0.8 649,572 + +0.8 649,647 - -0.0 649,649 - -0.5 649,649 - -0.3 649,652 + -0.8 649,652 + -0.8 649,652 + +0.3 649,652 + -0.3 649,753 + +0.4 649,754 - +0.3 649,754 - +0.5 649,819 - EX28DRAFT_0655 0.13 -0.2 649,992 + EX28DRAFT_0655 0.35 +0.1 650,213 + EX28DRAFT_0655 0.62 +0.4 650,214 - EX28DRAFT_0655 0.63 +0.7 650,232 + EX28DRAFT_0655 0.65 -0.1 650,430 - EX28DRAFT_0655 0.89 +1.5 650,462 + +0.4 650,463 - -0.1 650,499 + -1.3
Or see this region's nucleotide sequence