Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0405 and EX28DRAFT_0406 are separated by 155 nucleotides EX28DRAFT_0406 and EX28DRAFT_0407 are separated by 295 nucleotides
EX28DRAFT_0405: EX28DRAFT_0405 - Predicted membrane protein, at 381,746 to 382,903
_0405
EX28DRAFT_0406: EX28DRAFT_0406 - Transcriptional regulators, at 383,059 to 384,081
_0406
EX28DRAFT_0407: EX28DRAFT_0407 - ABC-type sugar transport system, periplasmic component, at 384,377 to 385,375
_0407
Position (kb) 383
384
385 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 382.063 kb on + strand, within EX28DRAFT_0405 at 382.128 kb on + strand, within EX28DRAFT_0405 at 382.128 kb on + strand, within EX28DRAFT_0405 at 382.141 kb on + strand, within EX28DRAFT_0405 at 382.160 kb on + strand, within EX28DRAFT_0405 at 382.161 kb on - strand, within EX28DRAFT_0405 at 382.161 kb on - strand, within EX28DRAFT_0405 at 382.161 kb on - strand, within EX28DRAFT_0405 at 382.521 kb on - strand, within EX28DRAFT_0405 at 382.601 kb on + strand, within EX28DRAFT_0405 at 382.656 kb on + strand, within EX28DRAFT_0405 at 382.679 kb on + strand, within EX28DRAFT_0405 at 382.783 kb on - strand, within EX28DRAFT_0405 at 382.786 kb on + strand, within EX28DRAFT_0405 at 382.817 kb on + strand at 382.817 kb on + strand at 382.818 kb on - strand at 383.007 kb on + strand at 383.059 kb on - strand at 383.059 kb on - strand at 383.166 kb on - strand, within EX28DRAFT_0406 at 383.264 kb on + strand, within EX28DRAFT_0406 at 383.264 kb on + strand, within EX28DRAFT_0406 at 383.265 kb on - strand, within EX28DRAFT_0406 at 383.265 kb on - strand, within EX28DRAFT_0406 at 383.329 kb on + strand, within EX28DRAFT_0406 at 383.346 kb on + strand, within EX28DRAFT_0406 at 383.347 kb on - strand, within EX28DRAFT_0406 at 383.347 kb on - strand, within EX28DRAFT_0406 at 383.347 kb on - strand, within EX28DRAFT_0406 at 383.351 kb on - strand, within EX28DRAFT_0406 at 383.406 kb on + strand, within EX28DRAFT_0406 at 383.461 kb on - strand, within EX28DRAFT_0406 at 383.619 kb on + strand, within EX28DRAFT_0406 at 383.620 kb on - strand, within EX28DRAFT_0406 at 383.772 kb on + strand, within EX28DRAFT_0406 at 383.772 kb on + strand, within EX28DRAFT_0406 at 383.789 kb on - strand, within EX28DRAFT_0406 at 383.858 kb on + strand, within EX28DRAFT_0406 at 383.859 kb on - strand, within EX28DRAFT_0406 at 384.129 kb on + strand at 384.150 kb on - strand at 384.184 kb on + strand at 384.185 kb on - strand at 384.207 kb on + strand at 384.282 kb on + strand at 384.373 kb on + strand at 384.382 kb on + strand at 384.382 kb on + strand at 384.383 kb on - strand at 384.473 kb on + strand at 384.475 kb on + strand at 384.475 kb on + strand at 384.475 kb on + strand at 384.476 kb on - strand at 384.476 kb on - strand at 384.476 kb on - strand at 384.479 kb on + strand, within EX28DRAFT_0407 at 384.479 kb on + strand, within EX28DRAFT_0407 at 384.749 kb on + strand, within EX28DRAFT_0407 at 384.750 kb on - strand, within EX28DRAFT_0407 at 384.750 kb on - strand, within EX28DRAFT_0407 at 384.762 kb on - strand, within EX28DRAFT_0407 at 384.764 kb on + strand, within EX28DRAFT_0407 at 384.764 kb on + strand, within EX28DRAFT_0407 at 384.765 kb on - strand, within EX28DRAFT_0407 at 384.796 kb on + strand, within EX28DRAFT_0407 at 384.797 kb on - strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.927 kb on - strand, within EX28DRAFT_0407 at 384.927 kb on - strand, within EX28DRAFT_0407 at 384.978 kb on + strand, within EX28DRAFT_0407 at 384.979 kb on - strand, within EX28DRAFT_0407 at 384.979 kb on - strand, within EX28DRAFT_0407
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 382,063 + EX28DRAFT_0405 0.27 -1.1 382,128 + EX28DRAFT_0405 0.33 +0.6 382,128 + EX28DRAFT_0405 0.33 +0.1 382,141 + EX28DRAFT_0405 0.34 +0.4 382,160 + EX28DRAFT_0405 0.36 -0.0 382,161 - EX28DRAFT_0405 0.36 +0.1 382,161 - EX28DRAFT_0405 0.36 -0.8 382,161 - EX28DRAFT_0405 0.36 -0.9 382,521 - EX28DRAFT_0405 0.67 -0.3 382,601 + EX28DRAFT_0405 0.74 +0.5 382,656 + EX28DRAFT_0405 0.79 +1.5 382,679 + EX28DRAFT_0405 0.81 -0.3 382,783 - EX28DRAFT_0405 0.90 -0.5 382,786 + EX28DRAFT_0405 0.90 -1.7 382,817 + -0.4 382,817 + -0.6 382,818 - -0.8 383,007 + +0.6 383,059 - -1.2 383,059 - -0.4 383,166 - EX28DRAFT_0406 0.10 +0.9 383,264 + EX28DRAFT_0406 0.20 +0.9 383,264 + EX28DRAFT_0406 0.20 -0.5 383,265 - EX28DRAFT_0406 0.20 -3.3 383,265 - EX28DRAFT_0406 0.20 +0.4 383,329 + EX28DRAFT_0406 0.26 -1.0 383,346 + EX28DRAFT_0406 0.28 -0.4 383,347 - EX28DRAFT_0406 0.28 +0.1 383,347 - EX28DRAFT_0406 0.28 +1.5 383,347 - EX28DRAFT_0406 0.28 +0.9 383,351 - EX28DRAFT_0406 0.29 +0.3 383,406 + EX28DRAFT_0406 0.34 -0.2 383,461 - EX28DRAFT_0406 0.39 -0.1 383,619 + EX28DRAFT_0406 0.55 -2.3 383,620 - EX28DRAFT_0406 0.55 +0.5 383,772 + EX28DRAFT_0406 0.70 -1.5 383,772 + EX28DRAFT_0406 0.70 +0.3 383,789 - EX28DRAFT_0406 0.71 -0.1 383,858 + EX28DRAFT_0406 0.78 +0.1 383,859 - EX28DRAFT_0406 0.78 -3.5 384,129 + -1.2 384,150 - +0.3 384,184 + -0.3 384,185 - -1.3 384,207 + -0.4 384,282 + +2.5 384,373 + +0.3 384,382 + -1.1 384,382 + +0.2 384,383 - +0.2 384,473 + -0.1 384,475 + -0.3 384,475 + -2.1 384,475 + -0.2 384,476 - +0.4 384,476 - -1.8 384,476 - +1.8 384,479 + EX28DRAFT_0407 0.10 +0.4 384,479 + EX28DRAFT_0407 0.10 +1.2 384,749 + EX28DRAFT_0407 0.37 -0.0 384,750 - EX28DRAFT_0407 0.37 -0.7 384,750 - EX28DRAFT_0407 0.37 +0.1 384,762 - EX28DRAFT_0407 0.39 +0.3 384,764 + EX28DRAFT_0407 0.39 +0.1 384,764 + EX28DRAFT_0407 0.39 -1.5 384,765 - EX28DRAFT_0407 0.39 -0.8 384,796 + EX28DRAFT_0407 0.42 -3.6 384,797 - EX28DRAFT_0407 0.42 +0.3 384,926 + EX28DRAFT_0407 0.55 -1.7 384,926 + EX28DRAFT_0407 0.55 +0.1 384,926 + EX28DRAFT_0407 0.55 +0.3 384,926 + EX28DRAFT_0407 0.55 -0.4 384,926 + EX28DRAFT_0407 0.55 -0.1 384,927 - EX28DRAFT_0407 0.55 -0.1 384,927 - EX28DRAFT_0407 0.55 -0.7 384,978 + EX28DRAFT_0407 0.60 +0.2 384,979 - EX28DRAFT_0407 0.60 +0.3 384,979 - EX28DRAFT_0407 0.60 +0.2
Or see this region's nucleotide sequence