Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0329 and EX28DRAFT_0330 are separated by 10 nucleotides EX28DRAFT_0330 and EX28DRAFT_0331 overlap by 4 nucleotides EX28DRAFT_0331 and EX28DRAFT_0332 overlap by 4 nucleotides EX28DRAFT_0332 and EX28DRAFT_0333 are separated by 76 nucleotides
EX28DRAFT_0329: EX28DRAFT_0329 - NADH-quinone oxidoreductase, chain I, at 291,244 to 291,786
_0329
EX28DRAFT_0330: EX28DRAFT_0330 - NADH:ubiquinone oxidoreductase subunit 6 (chain J), at 291,797 to 292,351
_0330
EX28DRAFT_0331: EX28DRAFT_0331 - NADH:ubiquinone oxidoreductase subunit 11 or 4L (chain K), at 292,348 to 292,650
_0331
EX28DRAFT_0332: EX28DRAFT_0332 - proton-translocating NADH-quinone oxidoreductase, chain L, at 292,647 to 294,488
_0332
EX28DRAFT_0333: EX28DRAFT_0333 - proton-translocating NADH-quinone oxidoreductase, chain M, at 294,565 to 296,094
_0333
Position (kb) 292
293
294
295 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 291.685 kb on + strand, within EX28DRAFT_0329 at 291.754 kb on - strand at 291.812 kb on + strand at 291.812 kb on + strand at 291.888 kb on - strand, within EX28DRAFT_0330 at 291.891 kb on + strand, within EX28DRAFT_0330 at 292.186 kb on + strand, within EX28DRAFT_0330 at 292.531 kb on + strand, within EX28DRAFT_0331 at 292.532 kb on - strand, within EX28DRAFT_0331 at 292.532 kb on - strand, within EX28DRAFT_0331 at 292.962 kb on + strand, within EX28DRAFT_0332 at 292.963 kb on - strand, within EX28DRAFT_0332 at 292.965 kb on - strand, within EX28DRAFT_0332 at 293.064 kb on + strand, within EX28DRAFT_0332 at 293.064 kb on + strand, within EX28DRAFT_0332 at 293.115 kb on + strand, within EX28DRAFT_0332 at 293.115 kb on + strand, within EX28DRAFT_0332 at 293.118 kb on + strand, within EX28DRAFT_0332 at 293.300 kb on + strand, within EX28DRAFT_0332 at 293.670 kb on - strand, within EX28DRAFT_0332 at 293.773 kb on - strand, within EX28DRAFT_0332 at 293.931 kb on - strand, within EX28DRAFT_0332 at 293.931 kb on - strand, within EX28DRAFT_0332 at 293.953 kb on + strand, within EX28DRAFT_0332 at 294.195 kb on - strand, within EX28DRAFT_0332 at 294.426 kb on + strand at 294.587 kb on + strand at 294.587 kb on + strand at 294.925 kb on - strand, within EX28DRAFT_0333 at 295.065 kb on + strand, within EX28DRAFT_0333 at 295.093 kb on + strand, within EX28DRAFT_0333 at 295.094 kb on - strand, within EX28DRAFT_0333
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 291,685 + EX28DRAFT_0329 0.81 -2.0 291,754 - -1.2 291,812 + +0.2 291,812 + -0.1 291,888 - EX28DRAFT_0330 0.16 -2.4 291,891 + EX28DRAFT_0330 0.17 -1.3 292,186 + EX28DRAFT_0330 0.70 -2.4 292,531 + EX28DRAFT_0331 0.60 -1.3 292,532 - EX28DRAFT_0331 0.61 -2.9 292,532 - EX28DRAFT_0331 0.61 -1.2 292,962 + EX28DRAFT_0332 0.17 -1.9 292,963 - EX28DRAFT_0332 0.17 -1.7 292,965 - EX28DRAFT_0332 0.17 -1.8 293,064 + EX28DRAFT_0332 0.23 -1.8 293,064 + EX28DRAFT_0332 0.23 -0.8 293,115 + EX28DRAFT_0332 0.25 -1.4 293,115 + EX28DRAFT_0332 0.25 -1.5 293,118 + EX28DRAFT_0332 0.26 -2.3 293,300 + EX28DRAFT_0332 0.35 -2.5 293,670 - EX28DRAFT_0332 0.56 -1.8 293,773 - EX28DRAFT_0332 0.61 -1.3 293,931 - EX28DRAFT_0332 0.70 -1.6 293,931 - EX28DRAFT_0332 0.70 -1.4 293,953 + EX28DRAFT_0332 0.71 -1.8 294,195 - EX28DRAFT_0332 0.84 -2.0 294,426 + -1.1 294,587 + -1.8 294,587 + -1.2 294,925 - EX28DRAFT_0333 0.24 -0.7 295,065 + EX28DRAFT_0333 0.33 -1.1 295,093 + EX28DRAFT_0333 0.35 -2.5 295,094 - EX28DRAFT_0333 0.35 -2.6
Or see this region's nucleotide sequence