Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_0324

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_0323 and EX28DRAFT_0324 are separated by 2 nucleotidesEX28DRAFT_0324 and EX28DRAFT_0325 overlap by 4 nucleotides EX28DRAFT_0323: EX28DRAFT_0323 - NADH (or F420H2) dehydrogenase, subunit C, at 283,838 to 285,640 _0323 EX28DRAFT_0324: EX28DRAFT_0324 - NADH-quinone oxidoreductase, E subunit, at 285,643 to 286,143 _0324 EX28DRAFT_0325: EX28DRAFT_0325 - NADH-quinone oxidoreductase, F subunit, at 286,140 to 287,477 _0325 Position (kb) 285 286 287Strain fitness (log2 ratio) -3 -2 -1 0 1at 284.802 kb on - strand, within EX28DRAFT_0323at 285.018 kb on - strand, within EX28DRAFT_0323at 285.228 kb on - strand, within EX28DRAFT_0323at 285.228 kb on - strand, within EX28DRAFT_0323at 285.464 kb on - strandat 285.467 kb on - strandat 285.605 kb on - strandat 285.619 kb on + strandat 285.809 kb on - strand, within EX28DRAFT_0324at 285.868 kb on + strand, within EX28DRAFT_0324at 286.047 kb on + strand, within EX28DRAFT_0324at 286.047 kb on + strand, within EX28DRAFT_0324at 286.671 kb on + strand, within EX28DRAFT_0325at 286.672 kb on - strand, within EX28DRAFT_0325at 286.700 kb on + strand, within EX28DRAFT_0325at 287.084 kb on + strand, within EX28DRAFT_0325

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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284,802 - EX28DRAFT_0323 0.53 -0.5
285,018 - EX28DRAFT_0323 0.65 -3.6
285,228 - EX28DRAFT_0323 0.77 -3.0
285,228 - EX28DRAFT_0323 0.77 -1.1
285,464 - +0.9
285,467 - -1.2
285,605 - -1.9
285,619 + -1.9
285,809 - EX28DRAFT_0324 0.33 -1.6
285,868 + EX28DRAFT_0324 0.45 -0.9
286,047 + EX28DRAFT_0324 0.81 -0.6
286,047 + EX28DRAFT_0324 0.81 +0.2
286,671 + EX28DRAFT_0325 0.40 -1.6
286,672 - EX28DRAFT_0325 0.40 -1.0
286,700 + EX28DRAFT_0325 0.42 -2.6
287,084 + EX28DRAFT_0325 0.71 -1.6

Or see this region's nucleotide sequence