Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0312 and EX28DRAFT_0313 are separated by 76 nucleotides EX28DRAFT_0313 and EX28DRAFT_0314 are separated by 337 nucleotides EX28DRAFT_0314 and EX28DRAFT_0315 are separated by 92 nucleotides
EX28DRAFT_0312: EX28DRAFT_0312 - phosphate acetyltransferase, at 270,741 to 272,882
_0312
EX28DRAFT_0313: EX28DRAFT_0313 - acetate kinase, at 272,959 to 274,161
_0313
EX28DRAFT_0314: EX28DRAFT_0314 - Uncharacterized protein conserved in bacteria, at 274,499 to 274,954
_0314
EX28DRAFT_0315: EX28DRAFT_0315 - Uncharacterized conserved protein, at 275,047 to 275,541
_0315
Position (kb) 272
273
274
275 Strain fitness (log2 ratio) -1
0
1
2 at 272.603 kb on - strand, within EX28DRAFT_0312 at 272.868 kb on - strand at 272.868 kb on - strand at 272.871 kb on - strand at 273.212 kb on + strand, within EX28DRAFT_0313 at 273.212 kb on + strand, within EX28DRAFT_0313 at 273.284 kb on + strand, within EX28DRAFT_0313 at 273.285 kb on - strand, within EX28DRAFT_0313 at 273.299 kb on + strand, within EX28DRAFT_0313 at 273.481 kb on - strand, within EX28DRAFT_0313 at 273.599 kb on + strand, within EX28DRAFT_0313 at 273.599 kb on + strand, within EX28DRAFT_0313 at 273.600 kb on - strand, within EX28DRAFT_0313 at 273.678 kb on - strand, within EX28DRAFT_0313 at 273.678 kb on - strand, within EX28DRAFT_0313 at 273.954 kb on + strand, within EX28DRAFT_0313 at 274.033 kb on - strand, within EX28DRAFT_0313 at 274.033 kb on - strand, within EX28DRAFT_0313 at 274.033 kb on - strand, within EX28DRAFT_0313 at 274.147 kb on + strand at 274.171 kb on + strand at 274.171 kb on + strand at 274.172 kb on - strand at 274.180 kb on + strand at 274.181 kb on - strand at 274.240 kb on - strand at 274.291 kb on - strand at 274.291 kb on - strand at 274.525 kb on - strand at 274.670 kb on - strand, within EX28DRAFT_0314 at 274.801 kb on + strand, within EX28DRAFT_0314 at 274.802 kb on - strand, within EX28DRAFT_0314 at 274.802 kb on - strand, within EX28DRAFT_0314 at 274.806 kb on + strand, within EX28DRAFT_0314 at 274.905 kb on + strand, within EX28DRAFT_0314 at 275.089 kb on + strand at 275.089 kb on + strand at 275.090 kb on - strand at 275.104 kb on - strand, within EX28DRAFT_0315 at 275.153 kb on + strand, within EX28DRAFT_0315
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 272,603 - EX28DRAFT_0312 0.87 -1.3 272,868 - -1.5 272,868 - -1.0 272,871 - +1.0 273,212 + EX28DRAFT_0313 0.21 -1.5 273,212 + EX28DRAFT_0313 0.21 -0.0 273,284 + EX28DRAFT_0313 0.27 +1.0 273,285 - EX28DRAFT_0313 0.27 -1.0 273,299 + EX28DRAFT_0313 0.28 -0.9 273,481 - EX28DRAFT_0313 0.43 -0.7 273,599 + EX28DRAFT_0313 0.53 -0.4 273,599 + EX28DRAFT_0313 0.53 -1.0 273,600 - EX28DRAFT_0313 0.53 -1.0 273,678 - EX28DRAFT_0313 0.60 +0.5 273,678 - EX28DRAFT_0313 0.60 +1.0 273,954 + EX28DRAFT_0313 0.83 +2.0 274,033 - EX28DRAFT_0313 0.89 +1.0 274,033 - EX28DRAFT_0313 0.89 +0.1 274,033 - EX28DRAFT_0313 0.89 -1.1 274,147 + -1.0 274,171 + -0.6 274,171 + +1.3 274,172 - -0.5 274,180 + +1.0 274,181 - +0.6 274,240 - +1.6 274,291 - -0.6 274,291 - +0.6 274,525 - -0.8 274,670 - EX28DRAFT_0314 0.38 -0.2 274,801 + EX28DRAFT_0314 0.66 -0.6 274,802 - EX28DRAFT_0314 0.66 +0.7 274,802 - EX28DRAFT_0314 0.66 +1.1 274,806 + EX28DRAFT_0314 0.67 +0.4 274,905 + EX28DRAFT_0314 0.89 +0.3 275,089 + -0.8 275,089 + +0.6 275,090 - +0.0 275,104 - EX28DRAFT_0315 0.12 -0.1 275,153 + EX28DRAFT_0315 0.21 -0.5
Or see this region's nucleotide sequence