Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0241 and EX28DRAFT_0242 are separated by 786 nucleotides EX28DRAFT_0242 and EX28DRAFT_0243 are separated by 38 nucleotides
EX28DRAFT_0241: EX28DRAFT_0241 - lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase, at 194,832 to 195,749
_0241
EX28DRAFT_0242: EX28DRAFT_0242 - type VI secretion protein, VC_A0107 family, at 196,536 to 197,033
_0242
EX28DRAFT_0243: EX28DRAFT_0243 - type VI secretion protein, EvpB/VC_A0108 family, at 197,072 to 198,610
_0243
Position (kb) 196
197
198 Strain fitness (log2 ratio) -1
0
1
2
3 at 195.545 kb on - strand, within EX28DRAFT_0241 at 195.679 kb on - strand at 195.698 kb on + strand at 195.698 kb on + strand at 195.699 kb on - strand at 195.756 kb on - strand at 195.763 kb on - strand at 195.763 kb on - strand at 195.775 kb on + strand at 195.782 kb on - strand at 195.900 kb on + strand at 195.901 kb on - strand at 195.911 kb on + strand at 195.912 kb on - strand at 195.916 kb on + strand at 195.924 kb on - strand at 195.924 kb on - strand at 196.445 kb on + strand at 196.595 kb on - strand, within EX28DRAFT_0242 at 196.806 kb on - strand, within EX28DRAFT_0242 at 196.919 kb on + strand, within EX28DRAFT_0242 at 197.079 kb on + strand at 197.080 kb on - strand at 197.141 kb on + strand at 197.142 kb on - strand at 197.196 kb on + strand at 197.446 kb on + strand, within EX28DRAFT_0243 at 197.488 kb on + strand, within EX28DRAFT_0243 at 197.489 kb on - strand, within EX28DRAFT_0243 at 197.565 kb on - strand, within EX28DRAFT_0243 at 197.565 kb on - strand, within EX28DRAFT_0243 at 197.565 kb on - strand, within EX28DRAFT_0243 at 197.565 kb on - strand, within EX28DRAFT_0243 at 197.577 kb on - strand, within EX28DRAFT_0243 at 197.577 kb on - strand, within EX28DRAFT_0243 at 197.581 kb on + strand, within EX28DRAFT_0243 at 197.692 kb on + strand, within EX28DRAFT_0243 at 197.692 kb on + strand, within EX28DRAFT_0243 at 197.692 kb on + strand, within EX28DRAFT_0243 at 197.693 kb on - strand, within EX28DRAFT_0243 at 197.693 kb on - strand, within EX28DRAFT_0243 at 197.767 kb on + strand, within EX28DRAFT_0243 at 197.768 kb on - strand, within EX28DRAFT_0243 at 197.768 kb on - strand, within EX28DRAFT_0243 at 197.778 kb on - strand, within EX28DRAFT_0243 at 197.778 kb on - strand, within EX28DRAFT_0243 at 197.778 kb on - strand, within EX28DRAFT_0243 at 197.796 kb on - strand, within EX28DRAFT_0243 at 197.837 kb on + strand, within EX28DRAFT_0243 at 197.915 kb on + strand, within EX28DRAFT_0243 at 197.915 kb on + strand, within EX28DRAFT_0243 at 197.915 kb on + strand, within EX28DRAFT_0243 at 197.915 kb on + strand, within EX28DRAFT_0243 at 197.916 kb on - strand, within EX28DRAFT_0243 at 197.916 kb on - strand, within EX28DRAFT_0243 at 197.916 kb on - strand, within EX28DRAFT_0243 at 197.952 kb on - strand, within EX28DRAFT_0243 at 197.952 kb on - strand, within EX28DRAFT_0243 at 197.962 kb on + strand, within EX28DRAFT_0243 at 197.962 kb on + strand, within EX28DRAFT_0243 at 197.963 kb on - strand, within EX28DRAFT_0243 at 197.998 kb on + strand, within EX28DRAFT_0243 at 197.999 kb on - strand, within EX28DRAFT_0243
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 195,545 - EX28DRAFT_0241 0.78 -0.1 195,679 - +0.3 195,698 + +2.3 195,698 + +0.1 195,699 - -1.4 195,756 - -0.7 195,763 - +0.5 195,763 - +0.4 195,775 + +0.1 195,782 - +0.1 195,900 + -0.1 195,901 - -0.2 195,911 + -0.1 195,912 - -0.6 195,916 + -0.2 195,924 - -0.3 195,924 - -0.1 196,445 + -0.7 196,595 - EX28DRAFT_0242 0.12 +1.0 196,806 - EX28DRAFT_0242 0.54 -0.7 196,919 + EX28DRAFT_0242 0.77 -0.6 197,079 + -0.4 197,080 - +1.7 197,141 + -0.5 197,142 - -0.2 197,196 + +0.4 197,446 + EX28DRAFT_0243 0.24 +0.9 197,488 + EX28DRAFT_0243 0.27 -0.1 197,489 - EX28DRAFT_0243 0.27 +0.8 197,565 - EX28DRAFT_0243 0.32 -0.9 197,565 - EX28DRAFT_0243 0.32 -0.0 197,565 - EX28DRAFT_0243 0.32 -0.2 197,565 - EX28DRAFT_0243 0.32 +1.3 197,577 - EX28DRAFT_0243 0.33 +0.2 197,577 - EX28DRAFT_0243 0.33 +0.4 197,581 + EX28DRAFT_0243 0.33 +0.1 197,692 + EX28DRAFT_0243 0.40 -1.5 197,692 + EX28DRAFT_0243 0.40 -0.3 197,692 + EX28DRAFT_0243 0.40 -0.3 197,693 - EX28DRAFT_0243 0.40 -0.1 197,693 - EX28DRAFT_0243 0.40 +3.3 197,767 + EX28DRAFT_0243 0.45 -0.5 197,768 - EX28DRAFT_0243 0.45 -0.1 197,768 - EX28DRAFT_0243 0.45 -0.6 197,778 - EX28DRAFT_0243 0.46 -0.5 197,778 - EX28DRAFT_0243 0.46 +1.2 197,778 - EX28DRAFT_0243 0.46 -0.1 197,796 - EX28DRAFT_0243 0.47 -0.3 197,837 + EX28DRAFT_0243 0.50 -1.2 197,915 + EX28DRAFT_0243 0.55 +0.3 197,915 + EX28DRAFT_0243 0.55 +0.4 197,915 + EX28DRAFT_0243 0.55 +1.3 197,915 + EX28DRAFT_0243 0.55 +0.8 197,916 - EX28DRAFT_0243 0.55 -0.3 197,916 - EX28DRAFT_0243 0.55 +0.4 197,916 - EX28DRAFT_0243 0.55 -0.6 197,952 - EX28DRAFT_0243 0.57 +0.0 197,952 - EX28DRAFT_0243 0.57 +0.2 197,962 + EX28DRAFT_0243 0.58 -0.8 197,962 + EX28DRAFT_0243 0.58 +1.2 197,963 - EX28DRAFT_0243 0.58 -0.1 197,998 + EX28DRAFT_0243 0.60 +0.4 197,999 - EX28DRAFT_0243 0.60 +1.0
Or see this region's nucleotide sequence