Strain Fitness in Escherichia coli BW25113 around b2530

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthscB and iscA are separated by 95 nucleotidesiscA and iscU are separated by 16 nucleotidesiscU and iscS are separated by 27 nucleotidesiscS and iscR are separated by 111 nucleotides b2527: hscB - co-chaperone HscB (NCBI), at 2,656,974 to 2,657,489 hscB b2528: iscA - iron-sulfur cluster assembly protein (NCBI), at 2,657,585 to 2,657,908 iscA b2529: iscU - scaffold protein (NCBI), at 2,657,925 to 2,658,311 iscU b2530: iscS - cysteine desulfurase (NCBI), at 2,658,339 to 2,659,553 iscS b2531: iscR - DNA-binding transcriptional repressor (NCBI), at 2,659,665 to 2,660,153 iscR Position (kb) 2658 2659 2660Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 2657.633 kb on - strand, within iscAat 2657.795 kb on - strand, within iscAat 2657.873 kb on - strand, within iscAat 2657.886 kb on - strandat 2658.370 kb on + strandat 2659.636 kb on - strandat 2659.636 kb on - strandat 2659.684 kb on - strandat 2659.834 kb on - strand, within iscRat 2659.875 kb on - strand, within iscRat 2659.897 kb on - strand, within iscRat 2660.133 kb on - strandat 2660.376 kb on + strandat 2660.376 kb on + strandat 2660.376 kb on + strandat 2660.376 kb on + strandat 2660.475 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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2,657,633 - iscA b2528 0.15 -1.6
2,657,795 - iscA b2528 0.65 +0.1
2,657,873 - iscA b2528 0.89 -1.3
2,657,886 - -0.7
2,658,370 + -0.2
2,659,636 - -0.7
2,659,636 - -0.8
2,659,684 - -0.4
2,659,834 - iscR b2531 0.35 +4.8
2,659,875 - iscR b2531 0.43 +4.3
2,659,897 - iscR b2531 0.47 +4.5
2,660,133 - +4.5
2,660,376 + +0.0
2,660,376 + +0.8
2,660,376 + +1.2
2,660,376 + +0.5
2,660,475 - -0.7

Or see this region's nucleotide sequence