Strain Fitness in Escherichia coli BW25113 around b3991

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiH and thiG overlap by 4 nucleotidesthiG and thiS are separated by 1 nucleotidesthiS and thiF overlap by 17 nucleotidesthiF and thiE overlap by 8 nucleotides b3990: thiH - thiamine biosynthesis protein ThiH (NCBI), at 4,188,758 to 4,189,891 thiH b3991: thiG - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,189,888 to 4,190,658 thiG b4407: thiS - sulfur carrier protein ThiS (NCBI), at 4,190,660 to 4,190,860 thiS b3992: thiF - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,190,844 to 4,191,599 thiF b3993: thiE - thiamine-phosphate pyrophosphorylase (NCBI), at 4,191,592 to 4,192,227 thiE Position (kb) 4189 4190 4191Strain fitness (log2 ratio) -3 -2 -1 0 1at 4188.899 kb on - strand, within thiHat 4189.035 kb on - strand, within thiHat 4189.048 kb on + strand, within thiHat 4189.056 kb on - strand, within thiHat 4189.073 kb on - strand, within thiHat 4189.133 kb on - strand, within thiHat 4189.334 kb on - strand, within thiHat 4189.351 kb on - strand, within thiHat 4189.351 kb on - strand, within thiHat 4189.362 kb on - strand, within thiHat 4189.372 kb on - strand, within thiHat 4189.372 kb on - strand, within thiHat 4189.429 kb on + strand, within thiHat 4189.429 kb on - strand, within thiHat 4189.429 kb on - strand, within thiHat 4189.495 kb on + strand, within thiHat 4189.506 kb on + strand, within thiHat 4189.508 kb on + strand, within thiHat 4189.543 kb on - strand, within thiHat 4189.543 kb on - strand, within thiHat 4189.724 kb on + strand, within thiHat 4189.737 kb on - strand, within thiHat 4189.737 kb on - strand, within thiHat 4189.759 kb on - strand, within thiHat 4189.759 kb on - strand, within thiHat 4190.005 kb on - strand, within thiGat 4190.005 kb on - strand, within thiGat 4190.187 kb on - strand, within thiGat 4190.187 kb on - strand, within thiGat 4190.253 kb on + strand, within thiGat 4190.267 kb on - strand, within thiGat 4190.267 kb on - strand, within thiGat 4190.270 kb on + strand, within thiGat 4190.270 kb on + strand, within thiGat 4190.278 kb on - strand, within thiGat 4190.278 kb on - strand, within thiGat 4190.287 kb on - strand, within thiGat 4190.306 kb on - strand, within thiGat 4190.306 kb on - strand, within thiGat 4190.404 kb on + strand, within thiGat 4190.412 kb on - strand, within thiGat 4190.528 kb on - strand, within thiGat 4190.579 kb on + strand, within thiGat 4190.581 kb on + strand, within thiGat 4190.581 kb on + strand, within thiGat 4190.584 kb on - strandat 4190.586 kb on - strandat 4190.791 kb on + strand, within thiSat 4190.820 kb on + strand, within thiSat 4190.830 kb on + strand, within thiSat 4190.860 kb on - strandat 4190.908 kb on - strandat 4190.968 kb on - strand, within thiFat 4190.968 kb on - strand, within thiFat 4191.030 kb on + strand, within thiFat 4191.121 kb on + strand, within thiFat 4191.121 kb on + strand, within thiFat 4191.129 kb on - strand, within thiFat 4191.234 kb on + strand, within thiFat 4191.236 kb on - strand, within thiFat 4191.248 kb on + strand, within thiFat 4191.269 kb on + strand, within thiFat 4191.282 kb on - strand, within thiFat 4191.310 kb on - strand, within thiFat 4191.368 kb on - strand, within thiFat 4191.376 kb on + strand, within thiFat 4191.468 kb on - strand, within thiFat 4191.475 kb on - strand, within thiFat 4191.475 kb on - strand, within thiFat 4191.483 kb on - strand, within thiFat 4191.483 kb on - strand, within thiFat 4191.489 kb on + strand, within thiFat 4191.489 kb on + strand, within thiFat 4191.489 kb on + strand, within thiFat 4191.489 kb on + strand, within thiF

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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4,188,899 - thiH b3990 0.12 -0.1
4,189,035 - thiH b3990 0.24 -2.0
4,189,048 + thiH b3990 0.26 -3.1
4,189,056 - thiH b3990 0.26 -2.6
4,189,073 - thiH b3990 0.28 -0.9
4,189,133 - thiH b3990 0.33 -2.2
4,189,334 - thiH b3990 0.51 -0.0
4,189,351 - thiH b3990 0.52 -1.7
4,189,351 - thiH b3990 0.52 +0.4
4,189,362 - thiH b3990 0.53 -1.4
4,189,372 - thiH b3990 0.54 -0.4
4,189,372 - thiH b3990 0.54 -0.8
4,189,429 + thiH b3990 0.59 -2.5
4,189,429 - thiH b3990 0.59 -0.8
4,189,429 - thiH b3990 0.59 -1.1
4,189,495 + thiH b3990 0.65 -0.9
4,189,506 + thiH b3990 0.66 -3.3
4,189,508 + thiH b3990 0.66 -2.5
4,189,543 - thiH b3990 0.69 -2.6
4,189,543 - thiH b3990 0.69 -3.3
4,189,724 + thiH b3990 0.85 -1.1
4,189,737 - thiH b3990 0.86 +1.2
4,189,737 - thiH b3990 0.86 +0.8
4,189,759 - thiH b3990 0.88 -2.4
4,189,759 - thiH b3990 0.88 -0.1
4,190,005 - thiG b3991 0.15 -2.1
4,190,005 - thiG b3991 0.15 +0.0
4,190,187 - thiG b3991 0.39 +0.8
4,190,187 - thiG b3991 0.39 -1.2
4,190,253 + thiG b3991 0.47 -2.1
4,190,267 - thiG b3991 0.49 -1.9
4,190,267 - thiG b3991 0.49 -1.2
4,190,270 + thiG b3991 0.50 -0.6
4,190,270 + thiG b3991 0.50 -0.9
4,190,278 - thiG b3991 0.51 -1.6
4,190,278 - thiG b3991 0.51 -1.7
4,190,287 - thiG b3991 0.52 -3.7
4,190,306 - thiG b3991 0.54 -2.0
4,190,306 - thiG b3991 0.54 -1.4
4,190,404 + thiG b3991 0.67 -0.4
4,190,412 - thiG b3991 0.68 -2.1
4,190,528 - thiG b3991 0.83 -1.8
4,190,579 + thiG b3991 0.90 -3.0
4,190,581 + thiG b3991 0.90 -0.4
4,190,581 + thiG b3991 0.90 -1.9
4,190,584 - -0.2
4,190,586 - -2.0
4,190,791 + thiS b4407 0.65 -0.0
4,190,820 + thiS b4407 0.80 -0.4
4,190,830 + thiS b4407 0.85 -1.4
4,190,860 - -0.6
4,190,908 - -1.5
4,190,968 - thiF b3992 0.16 -2.6
4,190,968 - thiF b3992 0.16 -1.8
4,191,030 + thiF b3992 0.25 -1.5
4,191,121 + thiF b3992 0.37 -2.0
4,191,121 + thiF b3992 0.37 -1.2
4,191,129 - thiF b3992 0.38 -1.5
4,191,234 + thiF b3992 0.52 -0.0
4,191,236 - thiF b3992 0.52 -1.9
4,191,248 + thiF b3992 0.53 +0.1
4,191,269 + thiF b3992 0.56 -0.8
4,191,282 - thiF b3992 0.58 -1.0
4,191,310 - thiF b3992 0.62 -0.5
4,191,368 - thiF b3992 0.69 -3.0
4,191,376 + thiF b3992 0.70 -2.7
4,191,468 - thiF b3992 0.83 -2.0
4,191,475 - thiF b3992 0.83 -2.3
4,191,475 - thiF b3992 0.83 -1.9
4,191,483 - thiF b3992 0.85 +0.1
4,191,483 - thiF b3992 0.85 -1.6
4,191,489 + thiF b3992 0.85 -2.7
4,191,489 + thiF b3992 0.85 -1.4
4,191,489 + thiF b3992 0.85 -1.9
4,191,489 + thiF b3992 0.85 -0.7

Or see this region's nucleotide sequence