Experiment: L-Malic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argA and recD are separated by 61 nucleotides recD and recB overlap by 1 nucleotides
b2818: argA - N-acetylglutamate synthase (NCBI), at 2,947,264 to 2,948,595
argA
b2819: recD - exonuclease V (RecBCD complex), alpha chain (NCBI), at 2,948,657 to 2,950,483
recD
b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025
recB
Position (kb)
2948
2949
2950
2951 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2947.748 kb on + strand, within argA at 2947.766 kb on + strand, within argA at 2947.782 kb on - strand, within argA at 2947.782 kb on - strand, within argA at 2947.783 kb on + strand, within argA at 2948.091 kb on + strand, within argA at 2948.357 kb on + strand, within argA at 2948.415 kb on - strand, within argA at 2948.474 kb on + strand at 2948.480 kb on - strand at 2948.485 kb on + strand at 2948.493 kb on - strand at 2948.557 kb on - strand at 2948.584 kb on + strand at 2948.592 kb on - strand at 2948.592 kb on - strand at 2948.630 kb on - strand at 2948.689 kb on - strand at 2948.698 kb on + strand at 2948.711 kb on - strand at 2948.975 kb on + strand, within recD at 2948.983 kb on - strand, within recD at 2948.985 kb on + strand, within recD at 2948.988 kb on - strand, within recD at 2949.122 kb on + strand, within recD at 2949.124 kb on + strand, within recD at 2949.124 kb on + strand, within recD at 2949.159 kb on + strand, within recD at 2949.159 kb on + strand, within recD at 2949.559 kb on - strand, within recD at 2949.564 kb on - strand, within recD at 2949.648 kb on + strand, within recD at 2949.648 kb on - strand, within recD at 2949.648 kb on - strand, within recD at 2949.658 kb on + strand, within recD at 2949.691 kb on - strand, within recD at 2949.691 kb on - strand, within recD at 2949.911 kb on - strand, within recD at 2950.492 kb on + strand at 2950.492 kb on + strand at 2950.500 kb on - strand at 2951.352 kb on - strand, within recB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) remove 2,947,748 + argA b2818 0.36 -4.2 2,947,766 + argA b2818 0.38 -2.0 2,947,782 - argA b2818 0.39 -2.8 2,947,782 - argA b2818 0.39 -3.8 2,947,783 + argA b2818 0.39 -3.3 2,948,091 + argA b2818 0.62 -1.3 2,948,357 + argA b2818 0.82 -2.1 2,948,415 - argA b2818 0.86 -2.8 2,948,474 + -1.7 2,948,480 - -2.2 2,948,485 + -1.6 2,948,493 - -3.9 2,948,557 - -2.1 2,948,584 + -3.5 2,948,592 - -1.5 2,948,592 - -2.1 2,948,630 - -0.4 2,948,689 - +0.5 2,948,698 + +0.0 2,948,711 - -0.0 2,948,975 + recD b2819 0.17 -0.9 2,948,983 - recD b2819 0.18 -0.1 2,948,985 + recD b2819 0.18 -0.9 2,948,988 - recD b2819 0.18 -1.2 2,949,122 + recD b2819 0.25 -0.7 2,949,124 + recD b2819 0.26 +0.1 2,949,124 + recD b2819 0.26 -1.9 2,949,159 + recD b2819 0.27 +1.0 2,949,159 + recD b2819 0.27 +0.1 2,949,559 - recD b2819 0.49 +0.2 2,949,564 - recD b2819 0.50 -0.4 2,949,648 + recD b2819 0.54 -0.7 2,949,648 - recD b2819 0.54 +0.0 2,949,648 - recD b2819 0.54 -0.4 2,949,658 + recD b2819 0.55 -0.5 2,949,691 - recD b2819 0.57 +1.9 2,949,691 - recD b2819 0.57 +1.9 2,949,911 - recD b2819 0.69 -0.6 2,950,492 + -0.3 2,950,492 + -2.0 2,950,500 - -1.3 2,951,352 - recB b2820 0.25 -2.2
Or see this region's nucleotide sequence