Strain Fitness in Escherichia coli BW25113 around b0596

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntentE and entB are separated by 13 nucleotidesentB and entA overlap by 1 nucleotidesentA and ybdB are separated by 2 nucleotidesybdB and cstA are separated by 180 nucleotides b0594: entE - 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI), at 625,293 to 626,903 entE b0595: entB - isochorismatase (NCBI), at 626,917 to 627,774 entB b0596: entA - 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI), at 627,774 to 628,520 entA b0597: ybdB - hypothetical protein (NCBI), at 628,523 to 628,936 ybdB b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222 cstA Position (kb) 627 628 629Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 626.851 kb on - strandat 626.896 kb on - strandat 626.908 kb on - strandat 626.908 kb on - strandat 626.937 kb on + strandat 626.937 kb on + strandat 627.113 kb on + strand, within entBat 627.113 kb on + strand, within entBat 627.128 kb on + strand, within entBat 627.128 kb on + strand, within entBat 627.146 kb on - strand, within entBat 627.146 kb on - strand, within entBat 627.146 kb on - strand, within entBat 627.146 kb on - strand, within entBat 627.216 kb on - strand, within entBat 627.216 kb on - strand, within entBat 627.367 kb on + strand, within entBat 627.437 kb on - strand, within entBat 627.437 kb on - strand, within entBat 627.550 kb on - strand, within entBat 627.610 kb on - strand, within entBat 627.619 kb on + strand, within entBat 627.619 kb on + strand, within entBat 627.629 kb on - strand, within entBat 627.706 kb on - strandat 627.751 kb on + strandat 627.825 kb on + strandat 627.950 kb on - strand, within entAat 628.176 kb on + strand, within entAat 628.200 kb on + strand, within entAat 628.208 kb on - strand, within entAat 628.219 kb on - strand, within entAat 628.257 kb on - strand, within entAat 628.287 kb on - strand, within entAat 628.287 kb on - strand, within entAat 628.297 kb on + strand, within entAat 628.297 kb on + strand, within entAat 628.319 kb on - strand, within entAat 628.387 kb on - strand, within entAat 628.463 kb on - strandat 628.463 kb on - strandat 628.601 kb on + strand, within ybdBat 628.609 kb on - strand, within ybdBat 628.618 kb on - strand, within ybdBat 628.640 kb on + strand, within ybdBat 628.640 kb on + strand, within ybdBat 628.756 kb on + strand, within ybdBat 628.756 kb on + strand, within ybdBat 628.790 kb on - strand, within ybdBat 628.923 kb on - strandat 628.923 kb on - strandat 629.097 kb on - strandat 629.097 kb on - strandat 629.099 kb on + strandat 629.100 kb on - strandat 629.140 kb on + strandat 629.140 kb on + strandat 629.140 kb on + strandat 629.182 kb on + strandat 629.189 kb on + strandat 629.189 kb on + strandat 629.275 kb on + strandat 629.275 kb on + strandat 629.330 kb on - strand, within cstAat 629.330 kb on - strand, within cstAat 629.333 kb on + strand, within cstAat 629.333 kb on + strand, within cstAat 629.435 kb on - strand, within cstAat 629.512 kb on - strand, within cstA

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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626,851 - +0.8
626,896 - +0.1
626,908 - +0.6
626,908 - -0.1
626,937 + -0.4
626,937 + +0.3
627,113 + entB b0595 0.23 +0.8
627,113 + entB b0595 0.23 +0.4
627,128 + entB b0595 0.25 -0.4
627,128 + entB b0595 0.25 -0.2
627,146 - entB b0595 0.27 -0.2
627,146 - entB b0595 0.27 +0.0
627,146 - entB b0595 0.27 +0.3
627,146 - entB b0595 0.27 +1.4
627,216 - entB b0595 0.35 +0.6
627,216 - entB b0595 0.35 -0.5
627,367 + entB b0595 0.52 +0.3
627,437 - entB b0595 0.61 +0.4
627,437 - entB b0595 0.61 +0.1
627,550 - entB b0595 0.74 +0.4
627,610 - entB b0595 0.81 +0.3
627,619 + entB b0595 0.82 +0.2
627,619 + entB b0595 0.82 -0.7
627,629 - entB b0595 0.83 +0.6
627,706 - +0.4
627,751 + -0.7
627,825 + +0.4
627,950 - entA b0596 0.24 +1.8
628,176 + entA b0596 0.54 +0.7
628,200 + entA b0596 0.57 +4.5
628,208 - entA b0596 0.58 -0.2
628,219 - entA b0596 0.60 -0.5
628,257 - entA b0596 0.65 +0.6
628,287 - entA b0596 0.69 +1.0
628,287 - entA b0596 0.69 +1.5
628,297 + entA b0596 0.70 -0.5
628,297 + entA b0596 0.70 +0.7
628,319 - entA b0596 0.73 -0.1
628,387 - entA b0596 0.82 +0.6
628,463 - +0.6
628,463 - -1.0
628,601 + ybdB b0597 0.19 +0.3
628,609 - ybdB b0597 0.21 +0.8
628,618 - ybdB b0597 0.23 +0.8
628,640 + ybdB b0597 0.28 -1.1
628,640 + ybdB b0597 0.28 -0.7
628,756 + ybdB b0597 0.56 +0.0
628,756 + ybdB b0597 0.56 -1.1
628,790 - ybdB b0597 0.64 +0.6
628,923 - +0.1
628,923 - +0.6
629,097 - -0.9
629,097 - -0.2
629,099 + -0.0
629,100 - +0.4
629,140 + -0.7
629,140 + -1.4
629,140 + -2.6
629,182 + -0.3
629,189 + +0.8
629,189 + +1.8
629,275 + -1.1
629,275 + -0.4
629,330 - cstA b0598 0.10 -0.4
629,330 - cstA b0598 0.10 -0.4
629,333 + cstA b0598 0.10 -1.9
629,333 + cstA b0598 0.10 -0.9
629,435 - cstA b0598 0.15 -1.6
629,512 - cstA b0598 0.19 -0.9

Or see this region's nucleotide sequence