Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_23495 and IAI46_23500 are separated by 370 nucleotides IAI46_23500 and IAI46_23505 are separated by 59 nucleotides IAI46_23505 and IAI46_23510 are separated by 121 nucleotides IAI46_23510 and IAI46_23515 are separated by 14 nucleotides
IAI46_23495: IAI46_23495 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 4,999,348 to 5,000,181
_23495
IAI46_23500: IAI46_23500 - SlyX family protein, at 5,000,552 to 5,000,779
_23500
IAI46_23505: IAI46_23505 - peptidylprolyl isomerase, at 5,000,839 to 5,001,435
_23505
IAI46_23510: IAI46_23510 - YheV family putative metal-binding protein, at 5,001,557 to 5,001,760
_23510
IAI46_23515: IAI46_23515 - glutathione-regulated potassium-efflux system protein KefB, at 5,001,775 to 5,003,583
_23515
Position (kb) 5000
5001
5002 Strain fitness (log2 ratio) -2
-1
0
1 at 5000.016 kb on - strand, within IAI46_23495 at 5000.016 kb on - strand, within IAI46_23495 at 5000.094 kb on + strand, within IAI46_23495 at 5000.094 kb on + strand, within IAI46_23495 at 5000.167 kb on - strand at 5000.222 kb on + strand at 5000.223 kb on - strand at 5000.223 kb on - strand at 5000.226 kb on + strand at 5000.226 kb on + strand at 5000.226 kb on + strand at 5000.226 kb on + strand at 5000.227 kb on - strand at 5000.227 kb on - strand at 5000.243 kb on + strand at 5000.243 kb on + strand at 5000.243 kb on + strand at 5000.243 kb on + strand at 5000.243 kb on + strand at 5000.243 kb on + strand at 5000.244 kb on - strand at 5000.244 kb on - strand at 5000.244 kb on - strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.245 kb on + strand at 5000.246 kb on - strand at 5000.246 kb on - strand at 5000.246 kb on - strand at 5000.246 kb on - strand at 5000.246 kb on - strand at 5000.246 kb on - strand at 5000.246 kb on - strand at 5000.312 kb on + strand at 5000.313 kb on - strand at 5000.348 kb on + strand at 5000.348 kb on + strand at 5000.348 kb on + strand at 5000.384 kb on + strand at 5000.506 kb on + strand at 5000.512 kb on - strand at 5000.512 kb on - strand at 5000.512 kb on - strand at 5000.545 kb on + strand at 5000.545 kb on + strand at 5000.545 kb on + strand at 5000.545 kb on + strand at 5000.546 kb on - strand at 5000.546 kb on - strand at 5000.630 kb on - strand, within IAI46_23500 at 5000.780 kb on + strand at 5000.780 kb on + strand at 5000.780 kb on + strand at 5000.781 kb on - strand at 5001.009 kb on + strand, within IAI46_23505 at 5001.034 kb on + strand, within IAI46_23505 at 5001.035 kb on - strand, within IAI46_23505 at 5001.085 kb on + strand, within IAI46_23505 at 5001.085 kb on + strand, within IAI46_23505 at 5001.183 kb on + strand, within IAI46_23505 at 5001.183 kb on + strand, within IAI46_23505 at 5001.184 kb on - strand, within IAI46_23505 at 5001.335 kb on + strand, within IAI46_23505 at 5001.387 kb on + strand at 5001.391 kb on + strand at 5001.391 kb on + strand at 5001.391 kb on + strand at 5001.392 kb on - strand at 5001.398 kb on + strand at 5001.399 kb on - strand at 5001.399 kb on - strand at 5001.399 kb on - strand at 5001.427 kb on + strand at 5001.438 kb on + strand at 5001.445 kb on + strand at 5001.455 kb on + strand at 5001.455 kb on + strand at 5001.455 kb on + strand at 5001.456 kb on - strand at 5001.456 kb on - strand at 5001.456 kb on - strand at 5001.503 kb on - strand at 5001.558 kb on + strand at 5001.697 kb on + strand, within IAI46_23510 at 5001.709 kb on + strand, within IAI46_23510 at 5001.710 kb on - strand, within IAI46_23510 at 5001.776 kb on + strand at 5001.782 kb on + strand at 5001.782 kb on + strand at 5001.927 kb on + strand at 5002.105 kb on - strand, within IAI46_23515 at 5002.145 kb on + strand, within IAI46_23515 at 5002.167 kb on + strand, within IAI46_23515 at 5002.168 kb on - strand, within IAI46_23515 at 5002.168 kb on - strand, within IAI46_23515 at 5002.241 kb on + strand, within IAI46_23515 at 5002.242 kb on - strand, within IAI46_23515 at 5002.242 kb on - strand, within IAI46_23515 at 5002.244 kb on + strand, within IAI46_23515 at 5002.244 kb on + strand, within IAI46_23515 at 5002.244 kb on + strand, within IAI46_23515 at 5002.245 kb on - strand, within IAI46_23515 at 5002.245 kb on - strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.251 kb on + strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.252 kb on - strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.253 kb on + strand, within IAI46_23515 at 5002.254 kb on - strand, within IAI46_23515 at 5002.254 kb on - strand, within IAI46_23515 at 5002.254 kb on - strand, within IAI46_23515 at 5002.254 kb on - strand, within IAI46_23515 at 5002.254 kb on - strand, within IAI46_23515 at 5002.260 kb on - strand, within IAI46_23515 at 5002.306 kb on - strand, within IAI46_23515 at 5002.308 kb on + strand, within IAI46_23515 at 5002.308 kb on + strand, within IAI46_23515 at 5002.308 kb on + strand, within IAI46_23515 at 5002.309 kb on - strand, within IAI46_23515 at 5002.309 kb on - strand, within IAI46_23515 at 5002.309 kb on - strand, within IAI46_23515 at 5002.309 kb on - strand, within IAI46_23515 at 5002.309 kb on - strand, within IAI46_23515 at 5002.309 kb on - strand, within IAI46_23515 at 5002.400 kb on + strand, within IAI46_23515 at 5002.400 kb on + strand, within IAI46_23515 at 5002.401 kb on - strand, within IAI46_23515 at 5002.401 kb on - strand, within IAI46_23515 at 5002.401 kb on - strand, within IAI46_23515 at 5002.434 kb on - strand, within IAI46_23515
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 5,000,016 - IAI46_23495 0.80 +0.1 5,000,016 - IAI46_23495 0.80 +1.0 5,000,094 + IAI46_23495 0.89 -0.1 5,000,094 + IAI46_23495 0.89 -1.0 5,000,167 - +0.3 5,000,222 + +0.2 5,000,223 - -0.3 5,000,223 - +0.4 5,000,226 + -0.1 5,000,226 + -0.1 5,000,226 + -0.3 5,000,226 + -0.4 5,000,227 - -1.6 5,000,227 - +0.4 5,000,243 + -0.3 5,000,243 + -0.2 5,000,243 + -0.0 5,000,243 + -0.4 5,000,243 + +0.5 5,000,243 + +0.6 5,000,244 - +0.4 5,000,244 - +1.7 5,000,244 - -0.1 5,000,245 + -0.3 5,000,245 + -2.5 5,000,245 + -0.3 5,000,245 + +0.1 5,000,245 + -0.2 5,000,245 + +0.0 5,000,245 + -0.3 5,000,245 + -0.1 5,000,245 + +0.4 5,000,245 + -0.0 5,000,245 + -0.3 5,000,246 - +0.1 5,000,246 - -0.8 5,000,246 - -0.5 5,000,246 - -0.2 5,000,246 - -0.1 5,000,246 - +0.5 5,000,246 - -0.2 5,000,312 + +1.3 5,000,313 - -0.0 5,000,348 + +0.6 5,000,348 + +0.3 5,000,348 + -0.8 5,000,384 + +0.6 5,000,506 + -0.5 5,000,512 - +0.2 5,000,512 - +0.5 5,000,512 - +0.3 5,000,545 + +0.4 5,000,545 + +0.7 5,000,545 + +0.5 5,000,545 + +0.4 5,000,546 - +0.0 5,000,546 - +0.4 5,000,630 - IAI46_23500 0.34 -0.4 5,000,780 + +0.0 5,000,780 + +0.3 5,000,780 + +0.3 5,000,781 - +0.6 5,001,009 + IAI46_23505 0.28 -0.9 5,001,034 + IAI46_23505 0.33 -1.5 5,001,035 - IAI46_23505 0.33 -0.3 5,001,085 + IAI46_23505 0.41 +0.9 5,001,085 + IAI46_23505 0.41 -0.7 5,001,183 + IAI46_23505 0.58 -0.3 5,001,183 + IAI46_23505 0.58 -1.1 5,001,184 - IAI46_23505 0.58 -1.3 5,001,335 + IAI46_23505 0.83 -0.6 5,001,387 + -0.5 5,001,391 + +0.6 5,001,391 + -0.1 5,001,391 + -1.7 5,001,392 - +0.3 5,001,398 + -0.5 5,001,399 - -0.4 5,001,399 - +0.4 5,001,399 - -0.3 5,001,427 + -0.9 5,001,438 + -0.3 5,001,445 + -0.1 5,001,455 + -0.1 5,001,455 + +0.9 5,001,455 + -0.5 5,001,456 - -0.0 5,001,456 - +0.1 5,001,456 - +0.2 5,001,503 - +0.9 5,001,558 + +0.3 5,001,697 + IAI46_23510 0.69 +0.0 5,001,709 + IAI46_23510 0.75 +0.1 5,001,710 - IAI46_23510 0.75 +0.5 5,001,776 + -0.1 5,001,782 + -0.8 5,001,782 + +1.7 5,001,927 + +0.1 5,002,105 - IAI46_23515 0.18 +0.1 5,002,145 + IAI46_23515 0.20 -2.2 5,002,167 + IAI46_23515 0.22 +0.2 5,002,168 - IAI46_23515 0.22 +0.4 5,002,168 - IAI46_23515 0.22 -0.7 5,002,241 + IAI46_23515 0.26 +0.6 5,002,242 - IAI46_23515 0.26 +0.1 5,002,242 - IAI46_23515 0.26 -0.0 5,002,244 + IAI46_23515 0.26 +0.9 5,002,244 + IAI46_23515 0.26 +1.3 5,002,244 + IAI46_23515 0.26 -0.1 5,002,245 - IAI46_23515 0.26 +1.0 5,002,245 - IAI46_23515 0.26 +0.1 5,002,251 + IAI46_23515 0.26 -0.2 5,002,251 + IAI46_23515 0.26 -1.3 5,002,251 + IAI46_23515 0.26 +0.2 5,002,251 + IAI46_23515 0.26 +0.1 5,002,251 + IAI46_23515 0.26 +0.3 5,002,251 + IAI46_23515 0.26 +1.2 5,002,251 + IAI46_23515 0.26 -0.6 5,002,251 + IAI46_23515 0.26 -0.0 5,002,251 + IAI46_23515 0.26 +0.3 5,002,251 + IAI46_23515 0.26 +0.1 5,002,252 - IAI46_23515 0.26 -0.3 5,002,252 - IAI46_23515 0.26 +0.4 5,002,252 - IAI46_23515 0.26 -0.2 5,002,252 - IAI46_23515 0.26 +0.3 5,002,252 - IAI46_23515 0.26 +0.2 5,002,252 - IAI46_23515 0.26 -0.5 5,002,252 - IAI46_23515 0.26 -0.3 5,002,252 - IAI46_23515 0.26 +1.1 5,002,252 - IAI46_23515 0.26 +0.2 5,002,253 + IAI46_23515 0.26 -0.3 5,002,253 + IAI46_23515 0.26 +0.8 5,002,253 + IAI46_23515 0.26 +0.8 5,002,253 + IAI46_23515 0.26 +0.6 5,002,253 + IAI46_23515 0.26 +0.0 5,002,253 + IAI46_23515 0.26 -0.1 5,002,253 + IAI46_23515 0.26 -0.1 5,002,253 + IAI46_23515 0.26 -0.1 5,002,253 + IAI46_23515 0.26 -0.9 5,002,254 - IAI46_23515 0.26 +0.5 5,002,254 - IAI46_23515 0.26 +0.6 5,002,254 - IAI46_23515 0.26 -0.2 5,002,254 - IAI46_23515 0.26 +0.6 5,002,254 - IAI46_23515 0.26 +1.2 5,002,260 - IAI46_23515 0.27 -1.5 5,002,306 - IAI46_23515 0.29 +1.5 5,002,308 + IAI46_23515 0.29 -0.5 5,002,308 + IAI46_23515 0.29 -1.9 5,002,308 + IAI46_23515 0.29 +0.3 5,002,309 - IAI46_23515 0.30 -1.3 5,002,309 - IAI46_23515 0.30 +0.4 5,002,309 - IAI46_23515 0.30 -0.3 5,002,309 - IAI46_23515 0.30 +0.6 5,002,309 - IAI46_23515 0.30 +0.9 5,002,309 - IAI46_23515 0.30 +0.5 5,002,400 + IAI46_23515 0.35 -1.0 5,002,400 + IAI46_23515 0.35 -1.3 5,002,401 - IAI46_23515 0.35 +0.9 5,002,401 - IAI46_23515 0.35 +0.4 5,002,401 - IAI46_23515 0.35 +0.3 5,002,434 - IAI46_23515 0.36 +0.4
Or see this region's nucleotide sequence