Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_18895 and IAI46_18900 are separated by 80 nucleotides IAI46_18900 and IAI46_18905 are separated by 28 nucleotides IAI46_18905 and IAI46_18910 overlap by 4 nucleotides
IAI46_18895: IAI46_18895 - spermidine N1-acetyltransferase, at 3,975,804 to 3,976,358
_18895
IAI46_18900: IAI46_18900 - phosphate ABC transporter ATP-binding protein, at 3,976,439 to 3,977,251
_18900
IAI46_18905: IAI46_18905 - phosphate ABC transporter permease PstA, at 3,977,280 to 3,978,932
_18905
IAI46_18910: IAI46_18910 - ABC transporter permease subunit, at 3,978,929 to 3,981,091
_18910
Position (kb)
3977
3978
3979 Strain fitness (log2 ratio)
-1
0
1
2 at 3976.308 kb on + strand at 3976.309 kb on - strand at 3976.309 kb on - strand at 3976.352 kb on + strand at 3976.390 kb on - strand at 3976.407 kb on + strand at 3976.408 kb on - strand at 3976.409 kb on + strand at 3976.409 kb on + strand at 3976.410 kb on - strand at 3976.410 kb on - strand at 3976.410 kb on - strand at 3976.447 kb on - strand at 3976.459 kb on + strand at 3976.459 kb on + strand at 3976.460 kb on - strand at 3976.461 kb on + strand at 3976.461 kb on + strand at 3976.461 kb on + strand at 3976.461 kb on + strand at 3976.462 kb on - strand at 3976.492 kb on + strand at 3976.492 kb on + strand at 3976.493 kb on - strand at 3976.493 kb on - strand at 3976.493 kb on - strand at 3976.515 kb on + strand at 3976.515 kb on + strand at 3976.606 kb on - strand, within IAI46_18900 at 3976.606 kb on - strand, within IAI46_18900 at 3976.947 kb on - strand, within IAI46_18900 at 3977.038 kb on + strand, within IAI46_18900 at 3977.038 kb on + strand, within IAI46_18900 at 3977.116 kb on + strand, within IAI46_18900 at 3977.188 kb on + strand at 3977.189 kb on - strand at 3977.252 kb on + strand at 3977.253 kb on - strand at 3977.269 kb on + strand at 3977.269 kb on + strand at 3977.269 kb on + strand at 3977.281 kb on + strand at 3977.299 kb on + strand at 3977.350 kb on - strand at 3977.352 kb on + strand at 3977.353 kb on - strand at 3977.434 kb on + strand at 3977.434 kb on + strand at 3977.434 kb on + strand at 3977.457 kb on - strand, within IAI46_18905 at 3977.489 kb on + strand, within IAI46_18905 at 3977.489 kb on + strand, within IAI46_18905 at 3977.490 kb on - strand, within IAI46_18905 at 3977.538 kb on + strand, within IAI46_18905 at 3977.621 kb on + strand, within IAI46_18905 at 3977.621 kb on + strand, within IAI46_18905 at 3977.622 kb on - strand, within IAI46_18905 at 3977.838 kb on - strand, within IAI46_18905 at 3977.838 kb on - strand, within IAI46_18905 at 3977.838 kb on - strand, within IAI46_18905 at 3977.839 kb on + strand, within IAI46_18905 at 3977.839 kb on + strand, within IAI46_18905 at 3977.840 kb on - strand, within IAI46_18905 at 3977.869 kb on + strand, within IAI46_18905 at 3977.870 kb on - strand, within IAI46_18905 at 3977.897 kb on + strand, within IAI46_18905 at 3977.933 kb on + strand, within IAI46_18905 at 3977.933 kb on + strand, within IAI46_18905 at 3977.944 kb on + strand, within IAI46_18905 at 3977.945 kb on - strand, within IAI46_18905 at 3977.956 kb on + strand, within IAI46_18905 at 3977.957 kb on - strand, within IAI46_18905 at 3978.177 kb on - strand, within IAI46_18905 at 3978.266 kb on - strand, within IAI46_18905 at 3978.321 kb on + strand, within IAI46_18905 at 3978.322 kb on - strand, within IAI46_18905 at 3978.322 kb on - strand, within IAI46_18905 at 3978.327 kb on - strand, within IAI46_18905 at 3978.340 kb on - strand, within IAI46_18905 at 3978.340 kb on - strand, within IAI46_18905 at 3978.366 kb on - strand, within IAI46_18905 at 3978.407 kb on + strand, within IAI46_18905 at 3978.408 kb on - strand, within IAI46_18905 at 3978.408 kb on - strand, within IAI46_18905 at 3978.468 kb on - strand, within IAI46_18905 at 3978.517 kb on + strand, within IAI46_18905 at 3978.524 kb on - strand, within IAI46_18905 at 3978.540 kb on - strand, within IAI46_18905 at 3978.542 kb on + strand, within IAI46_18905 at 3978.542 kb on + strand, within IAI46_18905 at 3978.542 kb on + strand, within IAI46_18905 at 3978.543 kb on - strand, within IAI46_18905 at 3978.601 kb on - strand, within IAI46_18905 at 3978.736 kb on + strand, within IAI46_18905 at 3978.737 kb on - strand, within IAI46_18905 at 3978.737 kb on - strand, within IAI46_18905 at 3978.737 kb on - strand, within IAI46_18905 at 3978.955 kb on - strand at 3978.955 kb on - strand at 3979.015 kb on + strand at 3979.015 kb on + strand at 3979.223 kb on - strand, within IAI46_18910 at 3979.253 kb on + strand, within IAI46_18910 at 3979.254 kb on - strand, within IAI46_18910 at 3979.348 kb on + strand, within IAI46_18910 at 3979.349 kb on - strand, within IAI46_18910 at 3979.407 kb on + strand, within IAI46_18910 at 3979.408 kb on - strand, within IAI46_18910 at 3979.408 kb on - strand, within IAI46_18910 at 3979.408 kb on - strand, within IAI46_18910 at 3979.408 kb on - strand, within IAI46_18910 at 3979.496 kb on + strand, within IAI46_18910 at 3979.630 kb on - strand, within IAI46_18910 at 3979.718 kb on - strand, within IAI46_18910 at 3979.718 kb on - strand, within IAI46_18910 at 3979.749 kb on + strand, within IAI46_18910 at 3979.758 kb on + strand, within IAI46_18910 at 3979.759 kb on - strand, within IAI46_18910 at 3979.759 kb on - strand, within IAI46_18910 at 3979.759 kb on - strand, within IAI46_18910 at 3979.804 kb on - strand, within IAI46_18910 at 3979.804 kb on - strand, within IAI46_18910 at 3979.804 kb on - strand, within IAI46_18910 at 3979.860 kb on + strand, within IAI46_18910 at 3979.893 kb on + strand, within IAI46_18910 at 3979.893 kb on + strand, within IAI46_18910 at 3979.894 kb on - strand, within IAI46_18910 at 3979.918 kb on - strand, within IAI46_18910 at 3979.918 kb on - strand, within IAI46_18910
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 3,976,308 + -0.7 3,976,309 - +1.7 3,976,309 - +0.3 3,976,352 + -0.0 3,976,390 - +0.2 3,976,407 + +0.7 3,976,408 - +0.3 3,976,409 + -0.2 3,976,409 + +0.6 3,976,410 - +0.3 3,976,410 - +0.8 3,976,410 - +1.2 3,976,447 - +0.2 3,976,459 + -0.4 3,976,459 + -0.2 3,976,460 - -0.1 3,976,461 + -0.5 3,976,461 + +0.1 3,976,461 + +0.0 3,976,461 + +0.4 3,976,462 - +0.9 3,976,492 + +0.9 3,976,492 + +0.7 3,976,493 - +0.1 3,976,493 - -0.8 3,976,493 - +0.1 3,976,515 + +0.2 3,976,515 + +0.8 3,976,606 - IAI46_18900 0.21 +0.5 3,976,606 - IAI46_18900 0.21 +0.5 3,976,947 - IAI46_18900 0.62 +1.3 3,977,038 + IAI46_18900 0.74 +1.3 3,977,038 + IAI46_18900 0.74 +0.1 3,977,116 + IAI46_18900 0.83 +0.6 3,977,188 + +0.7 3,977,189 - +0.7 3,977,252 + -0.2 3,977,253 - -0.1 3,977,269 + +0.2 3,977,269 + +0.9 3,977,269 + -0.4 3,977,281 + -0.1 3,977,299 + -0.1 3,977,350 - -1.3 3,977,352 + -0.7 3,977,353 - +1.0 3,977,434 + -0.9 3,977,434 + +0.6 3,977,434 + -0.0 3,977,457 - IAI46_18905 0.11 -0.6 3,977,489 + IAI46_18905 0.13 -1.3 3,977,489 + IAI46_18905 0.13 +0.9 3,977,490 - IAI46_18905 0.13 +0.6 3,977,538 + IAI46_18905 0.16 -0.3 3,977,621 + IAI46_18905 0.21 -0.1 3,977,621 + IAI46_18905 0.21 -0.4 3,977,622 - IAI46_18905 0.21 -1.5 3,977,838 - IAI46_18905 0.34 -0.3 3,977,838 - IAI46_18905 0.34 +0.8 3,977,838 - IAI46_18905 0.34 -0.9 3,977,839 + IAI46_18905 0.34 +0.3 3,977,839 + IAI46_18905 0.34 -0.1 3,977,840 - IAI46_18905 0.34 -0.2 3,977,869 + IAI46_18905 0.36 +0.0 3,977,870 - IAI46_18905 0.36 -0.4 3,977,897 + IAI46_18905 0.37 +0.8 3,977,933 + IAI46_18905 0.40 -1.5 3,977,933 + IAI46_18905 0.40 +0.6 3,977,944 + IAI46_18905 0.40 -0.4 3,977,945 - IAI46_18905 0.40 -0.7 3,977,956 + IAI46_18905 0.41 +0.3 3,977,957 - IAI46_18905 0.41 +0.7 3,978,177 - IAI46_18905 0.54 -0.1 3,978,266 - IAI46_18905 0.60 -1.2 3,978,321 + IAI46_18905 0.63 +0.8 3,978,322 - IAI46_18905 0.63 +0.6 3,978,322 - IAI46_18905 0.63 +1.9 3,978,327 - IAI46_18905 0.63 +0.2 3,978,340 - IAI46_18905 0.64 -0.1 3,978,340 - IAI46_18905 0.64 -0.0 3,978,366 - IAI46_18905 0.66 +1.1 3,978,407 + IAI46_18905 0.68 -0.4 3,978,408 - IAI46_18905 0.68 +1.8 3,978,408 - IAI46_18905 0.68 +0.7 3,978,468 - IAI46_18905 0.72 +0.2 3,978,517 + IAI46_18905 0.75 -0.6 3,978,524 - IAI46_18905 0.75 +0.6 3,978,540 - IAI46_18905 0.76 -0.4 3,978,542 + IAI46_18905 0.76 +0.1 3,978,542 + IAI46_18905 0.76 -0.3 3,978,542 + IAI46_18905 0.76 -0.5 3,978,543 - IAI46_18905 0.76 -0.2 3,978,601 - IAI46_18905 0.80 +0.3 3,978,736 + IAI46_18905 0.88 -0.2 3,978,737 - IAI46_18905 0.88 +0.5 3,978,737 - IAI46_18905 0.88 +0.5 3,978,737 - IAI46_18905 0.88 -0.7 3,978,955 - +0.2 3,978,955 - -0.2 3,979,015 + +0.1 3,979,015 + +0.0 3,979,223 - IAI46_18910 0.14 +0.6 3,979,253 + IAI46_18910 0.15 -0.4 3,979,254 - IAI46_18910 0.15 +0.6 3,979,348 + IAI46_18910 0.19 +0.4 3,979,349 - IAI46_18910 0.19 -0.9 3,979,407 + IAI46_18910 0.22 -0.2 3,979,408 - IAI46_18910 0.22 +1.3 3,979,408 - IAI46_18910 0.22 +0.1 3,979,408 - IAI46_18910 0.22 -0.0 3,979,408 - IAI46_18910 0.22 +0.0 3,979,496 + IAI46_18910 0.26 -0.5 3,979,630 - IAI46_18910 0.32 -0.6 3,979,718 - IAI46_18910 0.36 +0.5 3,979,718 - IAI46_18910 0.36 +0.2 3,979,749 + IAI46_18910 0.38 -0.4 3,979,758 + IAI46_18910 0.38 +0.2 3,979,759 - IAI46_18910 0.38 +0.8 3,979,759 - IAI46_18910 0.38 +1.0 3,979,759 - IAI46_18910 0.38 -0.2 3,979,804 - IAI46_18910 0.40 -1.1 3,979,804 - IAI46_18910 0.40 -0.3 3,979,804 - IAI46_18910 0.40 -0.2 3,979,860 + IAI46_18910 0.43 +0.3 3,979,893 + IAI46_18910 0.45 +0.3 3,979,893 + IAI46_18910 0.45 +0.4 3,979,894 - IAI46_18910 0.45 -0.6 3,979,918 - IAI46_18910 0.46 -1.5 3,979,918 - IAI46_18910 0.46 -0.1
Or see this region's nucleotide sequence