Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_15175 and IAI46_15180 overlap by 4 nucleotides IAI46_15180 and IAI46_15185 overlap by 4 nucleotides IAI46_15185 and IAI46_15190 are separated by 34 nucleotides IAI46_15190 and IAI46_15195 are separated by 114 nucleotides IAI46_15195 and IAI46_15200 are separated by 117 nucleotides
IAI46_15175: IAI46_15175 - DNA replication protein, at 3,187,082 to 3,187,795
_15175
IAI46_15180: IAI46_15180 - replication protein, at 3,187,792 to 3,188,700
_15180
IAI46_15185: IAI46_15185 - DNA-binding protein, at 3,188,697 to 3,189,530
_15185
IAI46_15190: IAI46_15190 - hypothetical protein, at 3,189,565 to 3,189,843
_15190
IAI46_15195: IAI46_15195 - helix-turn-helix domain-containing protein, at 3,189,958 to 3,190,185
_15195
IAI46_15200: IAI46_15200 - helix-turn-helix transcriptional regulator, at 3,190,303 to 3,191,037
_15200
Position (kb)
3188
3189
3190 Strain fitness (log2 ratio)
-2
-1
0
1 at 3187.796 kb on - strand at 3187.796 kb on - strand at 3187.796 kb on - strand at 3187.821 kb on + strand at 3187.866 kb on + strand at 3187.903 kb on - strand, within IAI46_15180 at 3187.924 kb on + strand, within IAI46_15180 at 3187.932 kb on + strand, within IAI46_15180 at 3187.933 kb on - strand, within IAI46_15180 at 3187.978 kb on + strand, within IAI46_15180 at 3188.086 kb on - strand, within IAI46_15180 at 3188.131 kb on - strand, within IAI46_15180 at 3188.232 kb on + strand, within IAI46_15180 at 3188.233 kb on - strand, within IAI46_15180 at 3188.263 kb on + strand, within IAI46_15180 at 3188.412 kb on + strand, within IAI46_15180 at 3188.457 kb on + strand, within IAI46_15180 at 3188.486 kb on - strand, within IAI46_15180 at 3188.525 kb on + strand, within IAI46_15180 at 3188.525 kb on + strand, within IAI46_15180 at 3188.526 kb on - strand, within IAI46_15180 at 3188.526 kb on - strand, within IAI46_15180 at 3188.526 kb on - strand, within IAI46_15180 at 3188.536 kb on + strand, within IAI46_15180 at 3188.536 kb on + strand, within IAI46_15180 at 3188.590 kb on + strand, within IAI46_15180 at 3188.591 kb on - strand, within IAI46_15180 at 3188.622 kb on - strand at 3188.644 kb on - strand at 3188.679 kb on + strand at 3188.791 kb on - strand, within IAI46_15185 at 3188.794 kb on + strand, within IAI46_15185 at 3188.842 kb on - strand, within IAI46_15185 at 3188.925 kb on - strand, within IAI46_15185 at 3188.925 kb on - strand, within IAI46_15185 at 3188.929 kb on - strand, within IAI46_15185 at 3189.048 kb on + strand, within IAI46_15185 at 3189.140 kb on - strand, within IAI46_15185 at 3189.182 kb on + strand, within IAI46_15185 at 3189.182 kb on + strand, within IAI46_15185 at 3189.182 kb on + strand, within IAI46_15185 at 3189.437 kb on - strand, within IAI46_15185 at 3189.460 kb on + strand at 3189.460 kb on + strand at 3189.460 kb on + strand at 3189.461 kb on - strand at 3189.476 kb on + strand at 3189.479 kb on + strand at 3189.480 kb on - strand at 3189.480 kb on - strand at 3189.535 kb on - strand at 3189.566 kb on + strand at 3189.610 kb on + strand, within IAI46_15190 at 3189.611 kb on - strand, within IAI46_15190 at 3189.611 kb on - strand, within IAI46_15190 at 3189.615 kb on - strand, within IAI46_15190 at 3189.870 kb on + strand at 3189.874 kb on + strand at 3189.981 kb on + strand, within IAI46_15195 at 3189.994 kb on + strand, within IAI46_15195 at 3189.994 kb on + strand, within IAI46_15195 at 3190.070 kb on - strand, within IAI46_15195 at 3190.081 kb on - strand, within IAI46_15195
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 3,187,796 - +0.5 3,187,796 - +1.1 3,187,796 - +0.7 3,187,821 + +0.1 3,187,866 + +0.2 3,187,903 - IAI46_15180 0.12 +1.1 3,187,924 + IAI46_15180 0.15 -1.5 3,187,932 + IAI46_15180 0.15 +0.1 3,187,933 - IAI46_15180 0.16 +0.5 3,187,978 + IAI46_15180 0.20 +1.3 3,188,086 - IAI46_15180 0.32 +1.4 3,188,131 - IAI46_15180 0.37 -0.3 3,188,232 + IAI46_15180 0.48 +0.1 3,188,233 - IAI46_15180 0.49 +0.8 3,188,263 + IAI46_15180 0.52 +0.0 3,188,412 + IAI46_15180 0.68 -0.1 3,188,457 + IAI46_15180 0.73 -0.8 3,188,486 - IAI46_15180 0.76 -0.4 3,188,525 + IAI46_15180 0.81 +0.5 3,188,525 + IAI46_15180 0.81 +0.5 3,188,526 - IAI46_15180 0.81 +0.1 3,188,526 - IAI46_15180 0.81 +0.4 3,188,526 - IAI46_15180 0.81 +1.0 3,188,536 + IAI46_15180 0.82 -0.1 3,188,536 + IAI46_15180 0.82 +0.1 3,188,590 + IAI46_15180 0.88 -1.0 3,188,591 - IAI46_15180 0.88 -0.0 3,188,622 - +0.5 3,188,644 - +1.0 3,188,679 + -1.3 3,188,791 - IAI46_15185 0.11 +0.8 3,188,794 + IAI46_15185 0.12 -0.5 3,188,842 - IAI46_15185 0.17 +0.7 3,188,925 - IAI46_15185 0.27 +0.4 3,188,925 - IAI46_15185 0.27 -0.5 3,188,929 - IAI46_15185 0.28 +0.7 3,189,048 + IAI46_15185 0.42 +0.5 3,189,140 - IAI46_15185 0.53 -0.1 3,189,182 + IAI46_15185 0.58 -0.9 3,189,182 + IAI46_15185 0.58 +0.9 3,189,182 + IAI46_15185 0.58 +0.7 3,189,437 - IAI46_15185 0.89 +0.6 3,189,460 + +0.1 3,189,460 + -0.1 3,189,460 + +0.1 3,189,461 - +0.4 3,189,476 + -0.2 3,189,479 + +0.4 3,189,480 - +0.6 3,189,480 - -2.1 3,189,535 - +0.1 3,189,566 + +0.1 3,189,610 + IAI46_15190 0.16 +0.3 3,189,611 - IAI46_15190 0.16 -0.9 3,189,611 - IAI46_15190 0.16 +0.5 3,189,615 - IAI46_15190 0.18 -0.5 3,189,870 + +0.5 3,189,874 + +0.0 3,189,981 + IAI46_15195 0.10 -0.4 3,189,994 + IAI46_15195 0.16 +0.4 3,189,994 + IAI46_15195 0.16 +1.5 3,190,070 - IAI46_15195 0.49 -0.5 3,190,081 - IAI46_15195 0.54 -1.0
Or see this region's nucleotide sequence