Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_04800 and IAI46_04805 are separated by 33 nucleotides IAI46_04805 and IAI46_04810 are separated by 132 nucleotides IAI46_04810 and IAI46_04815 are separated by 249 nucleotides
IAI46_04800: IAI46_04800 - proline-specific permease ProY, at 1,032,069 to 1,033,475
_04800
IAI46_04805: IAI46_04805 - hypothetical protein, at 1,033,509 to 1,033,772
_04805
IAI46_04810: IAI46_04810 - mechanosensitive ion channel, at 1,033,905 to 1,035,170
_04810
IAI46_04815: IAI46_04815 - maltodextrin glucosidase, at 1,035,420 to 1,037,243
_04815
Position (kb)
1033
1034
1035
1036 Strain fitness (log2 ratio)
-1
0
1 at 1032.917 kb on + strand, within IAI46_04800 at 1032.917 kb on + strand, within IAI46_04800 at 1032.947 kb on + strand, within IAI46_04800 at 1032.948 kb on - strand, within IAI46_04800 at 1033.128 kb on + strand, within IAI46_04800 at 1033.129 kb on - strand, within IAI46_04800 at 1033.129 kb on - strand, within IAI46_04800 at 1033.137 kb on + strand, within IAI46_04800 at 1033.137 kb on + strand, within IAI46_04800 at 1033.137 kb on + strand, within IAI46_04800 at 1033.138 kb on - strand, within IAI46_04800 at 1033.138 kb on - strand, within IAI46_04800 at 1033.138 kb on - strand, within IAI46_04800 at 1033.138 kb on - strand, within IAI46_04800 at 1033.138 kb on - strand, within IAI46_04800 at 1033.351 kb on - strand at 1033.419 kb on + strand at 1033.419 kb on + strand at 1033.419 kb on + strand at 1033.419 kb on + strand at 1033.420 kb on - strand at 1033.420 kb on - strand at 1033.420 kb on - strand at 1033.556 kb on + strand, within IAI46_04805 at 1033.743 kb on - strand, within IAI46_04805 at 1033.758 kb on + strand at 1033.806 kb on + strand at 1033.806 kb on + strand at 1033.806 kb on + strand at 1033.810 kb on - strand at 1033.810 kb on - strand at 1033.810 kb on - strand at 1033.821 kb on - strand at 1033.821 kb on - strand at 1033.847 kb on + strand at 1033.871 kb on + strand at 1033.875 kb on + strand at 1033.891 kb on + strand at 1033.891 kb on + strand at 1033.892 kb on - strand at 1033.892 kb on - strand at 1033.892 kb on - strand at 1033.943 kb on + strand at 1033.944 kb on - strand at 1034.013 kb on - strand at 1034.025 kb on - strand at 1034.025 kb on - strand at 1034.044 kb on + strand, within IAI46_04810 at 1034.045 kb on - strand, within IAI46_04810 at 1034.078 kb on - strand, within IAI46_04810 at 1034.135 kb on + strand, within IAI46_04810 at 1034.136 kb on - strand, within IAI46_04810 at 1034.136 kb on - strand, within IAI46_04810 at 1034.136 kb on - strand, within IAI46_04810 at 1034.136 kb on - strand, within IAI46_04810 at 1034.167 kb on + strand, within IAI46_04810 at 1034.167 kb on + strand, within IAI46_04810 at 1034.168 kb on - strand, within IAI46_04810 at 1034.168 kb on - strand, within IAI46_04810 at 1034.180 kb on - strand, within IAI46_04810 at 1034.239 kb on - strand, within IAI46_04810 at 1034.256 kb on - strand, within IAI46_04810 at 1034.256 kb on - strand, within IAI46_04810 at 1034.256 kb on - strand, within IAI46_04810 at 1034.311 kb on - strand, within IAI46_04810 at 1034.349 kb on + strand, within IAI46_04810 at 1034.350 kb on - strand, within IAI46_04810 at 1034.534 kb on - strand, within IAI46_04810 at 1034.576 kb on + strand, within IAI46_04810 at 1034.927 kb on + strand, within IAI46_04810 at 1034.928 kb on - strand, within IAI46_04810 at 1034.974 kb on + strand, within IAI46_04810 at 1035.156 kb on + strand at 1035.156 kb on + strand at 1035.182 kb on + strand at 1035.228 kb on + strand at 1035.244 kb on + strand at 1035.269 kb on + strand at 1035.273 kb on + strand at 1035.273 kb on + strand at 1035.273 kb on + strand at 1035.293 kb on + strand at 1035.293 kb on + strand at 1035.293 kb on + strand at 1035.402 kb on + strand at 1035.496 kb on + strand at 1035.530 kb on - strand at 1035.849 kb on + strand, within IAI46_04815 at 1035.933 kb on + strand, within IAI46_04815 at 1035.933 kb on + strand, within IAI46_04815 at 1035.933 kb on + strand, within IAI46_04815 at 1035.972 kb on + strand, within IAI46_04815 at 1036.002 kb on + strand, within IAI46_04815 at 1036.002 kb on + strand, within IAI46_04815 at 1036.003 kb on - strand, within IAI46_04815 at 1036.037 kb on + strand, within IAI46_04815 at 1036.038 kb on - strand, within IAI46_04815 at 1036.038 kb on - strand, within IAI46_04815 at 1036.042 kb on + strand, within IAI46_04815 at 1036.042 kb on - strand, within IAI46_04815 at 1036.042 kb on - strand, within IAI46_04815 at 1036.042 kb on - strand, within IAI46_04815 at 1036.042 kb on - strand, within IAI46_04815 at 1036.056 kb on + strand, within IAI46_04815 at 1036.056 kb on + strand, within IAI46_04815 at 1036.056 kb on + strand, within IAI46_04815 at 1036.056 kb on + strand, within IAI46_04815 at 1036.056 kb on + strand, within IAI46_04815 at 1036.057 kb on - strand, within IAI46_04815 at 1036.057 kb on - strand, within IAI46_04815 at 1036.057 kb on - strand, within IAI46_04815 at 1036.059 kb on + strand, within IAI46_04815 at 1036.059 kb on + strand, within IAI46_04815 at 1036.157 kb on + strand, within IAI46_04815
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 1,032,917 + IAI46_04800 0.60 +0.0 1,032,917 + IAI46_04800 0.60 -0.6 1,032,947 + IAI46_04800 0.62 -0.6 1,032,948 - IAI46_04800 0.62 -0.4 1,033,128 + IAI46_04800 0.75 +1.0 1,033,129 - IAI46_04800 0.75 -0.2 1,033,129 - IAI46_04800 0.75 -0.5 1,033,137 + IAI46_04800 0.76 -1.0 1,033,137 + IAI46_04800 0.76 +1.1 1,033,137 + IAI46_04800 0.76 -0.0 1,033,138 - IAI46_04800 0.76 +0.1 1,033,138 - IAI46_04800 0.76 -0.6 1,033,138 - IAI46_04800 0.76 +0.5 1,033,138 - IAI46_04800 0.76 +0.6 1,033,138 - IAI46_04800 0.76 +0.9 1,033,351 - -0.4 1,033,419 + +0.1 1,033,419 + +0.3 1,033,419 + -0.1 1,033,419 + -0.2 1,033,420 - -0.4 1,033,420 - -1.0 1,033,420 - -0.5 1,033,556 + IAI46_04805 0.18 +0.6 1,033,743 - IAI46_04805 0.89 +0.4 1,033,758 + +0.5 1,033,806 + -0.0 1,033,806 + +0.2 1,033,806 + -0.2 1,033,810 - +0.5 1,033,810 - +0.0 1,033,810 - +0.2 1,033,821 - -0.0 1,033,821 - +0.0 1,033,847 + -0.0 1,033,871 + +0.7 1,033,875 + -0.2 1,033,891 + -1.2 1,033,891 + +0.4 1,033,892 - -0.2 1,033,892 - -0.0 1,033,892 - +0.2 1,033,943 + +0.5 1,033,944 - -1.3 1,034,013 - -0.1 1,034,025 - +0.1 1,034,025 - -0.1 1,034,044 + IAI46_04810 0.11 +0.5 1,034,045 - IAI46_04810 0.11 +1.6 1,034,078 - IAI46_04810 0.14 -0.1 1,034,135 + IAI46_04810 0.18 -0.1 1,034,136 - IAI46_04810 0.18 +0.1 1,034,136 - IAI46_04810 0.18 +0.5 1,034,136 - IAI46_04810 0.18 +0.3 1,034,136 - IAI46_04810 0.18 +0.5 1,034,167 + IAI46_04810 0.21 -0.7 1,034,167 + IAI46_04810 0.21 +0.1 1,034,168 - IAI46_04810 0.21 +1.7 1,034,168 - IAI46_04810 0.21 -0.4 1,034,180 - IAI46_04810 0.22 +0.0 1,034,239 - IAI46_04810 0.26 +0.4 1,034,256 - IAI46_04810 0.28 +0.0 1,034,256 - IAI46_04810 0.28 +0.6 1,034,256 - IAI46_04810 0.28 +0.7 1,034,311 - IAI46_04810 0.32 -0.0 1,034,349 + IAI46_04810 0.35 +0.2 1,034,350 - IAI46_04810 0.35 +0.0 1,034,534 - IAI46_04810 0.50 -0.2 1,034,576 + IAI46_04810 0.53 -0.7 1,034,927 + IAI46_04810 0.81 +0.9 1,034,928 - IAI46_04810 0.81 +0.6 1,034,974 + IAI46_04810 0.84 +0.4 1,035,156 + +0.3 1,035,156 + -0.7 1,035,182 + -0.5 1,035,228 + -0.5 1,035,244 + +0.6 1,035,269 + -0.2 1,035,273 + +0.3 1,035,273 + -0.5 1,035,273 + +0.8 1,035,293 + +0.6 1,035,293 + -0.7 1,035,293 + +0.0 1,035,402 + -1.1 1,035,496 + +0.3 1,035,530 - -0.7 1,035,849 + IAI46_04815 0.24 +1.1 1,035,933 + IAI46_04815 0.28 -0.1 1,035,933 + IAI46_04815 0.28 +0.4 1,035,933 + IAI46_04815 0.28 -0.9 1,035,972 + IAI46_04815 0.30 +0.1 1,036,002 + IAI46_04815 0.32 -0.2 1,036,002 + IAI46_04815 0.32 +0.2 1,036,003 - IAI46_04815 0.32 -0.1 1,036,037 + IAI46_04815 0.34 -0.2 1,036,038 - IAI46_04815 0.34 +0.2 1,036,038 - IAI46_04815 0.34 -1.3 1,036,042 + IAI46_04815 0.34 -0.0 1,036,042 - IAI46_04815 0.34 -1.0 1,036,042 - IAI46_04815 0.34 -0.2 1,036,042 - IAI46_04815 0.34 -0.2 1,036,042 - IAI46_04815 0.34 -0.6 1,036,056 + IAI46_04815 0.35 -0.2 1,036,056 + IAI46_04815 0.35 -0.3 1,036,056 + IAI46_04815 0.35 -1.4 1,036,056 + IAI46_04815 0.35 +0.6 1,036,056 + IAI46_04815 0.35 +0.7 1,036,057 - IAI46_04815 0.35 +0.1 1,036,057 - IAI46_04815 0.35 +0.9 1,036,057 - IAI46_04815 0.35 -0.1 1,036,059 + IAI46_04815 0.35 +0.8 1,036,059 + IAI46_04815 0.35 +1.2 1,036,157 + IAI46_04815 0.40 +0.8
Or see this region's nucleotide sequence