Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_01730 and IAI46_01735 are separated by 43 nucleotides IAI46_01735 and IAI46_01740 are separated by 119 nucleotides IAI46_01740 and IAI46_01745 are separated by 256 nucleotides IAI46_01745 and IAI46_01750 are separated by 1 nucleotides
IAI46_01730: IAI46_01730 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 374,182 to 374,802
_01730
IAI46_01735: IAI46_01735 - iron-sulfur cluster repair protein YtfE, at 374,846 to 375,511
_01735
IAI46_01740: IAI46_01740 - bifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase, at 375,631 to 377,583
_01740
IAI46_01745: IAI46_01745 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 377,840 to 378,580
_01745
IAI46_01750: IAI46_01750 - YtfJ family protein, at 378,582 to 379,139
_01750
Position (kb)
375
376
377
378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 374.701 kb on + strand, within IAI46_01730 at 374.701 kb on + strand, within IAI46_01730 at 374.701 kb on + strand, within IAI46_01730 at 374.701 kb on + strand, within IAI46_01730 at 374.701 kb on + strand, within IAI46_01730 at 374.701 kb on + strand, within IAI46_01730 at 374.702 kb on - strand, within IAI46_01730 at 374.702 kb on - strand, within IAI46_01730 at 374.702 kb on - strand, within IAI46_01730 at 374.702 kb on - strand, within IAI46_01730 at 374.703 kb on + strand, within IAI46_01730 at 374.704 kb on - strand, within IAI46_01730 at 374.848 kb on - strand at 374.917 kb on + strand, within IAI46_01735 at 374.918 kb on - strand, within IAI46_01735 at 374.937 kb on + strand, within IAI46_01735 at 374.937 kb on + strand, within IAI46_01735 at 374.937 kb on + strand, within IAI46_01735 at 374.937 kb on + strand, within IAI46_01735 at 374.937 kb on + strand, within IAI46_01735 at 374.938 kb on - strand, within IAI46_01735 at 374.954 kb on - strand, within IAI46_01735 at 375.274 kb on + strand, within IAI46_01735 at 375.274 kb on + strand, within IAI46_01735 at 375.274 kb on + strand, within IAI46_01735 at 375.274 kb on + strand, within IAI46_01735 at 375.275 kb on - strand, within IAI46_01735 at 375.277 kb on - strand, within IAI46_01735 at 375.277 kb on - strand, within IAI46_01735 at 375.277 kb on - strand, within IAI46_01735 at 375.277 kb on - strand, within IAI46_01735 at 375.305 kb on - strand, within IAI46_01735 at 375.305 kb on - strand, within IAI46_01735 at 375.316 kb on - strand, within IAI46_01735 at 375.316 kb on - strand, within IAI46_01735 at 375.316 kb on - strand, within IAI46_01735 at 375.316 kb on - strand, within IAI46_01735 at 375.382 kb on + strand, within IAI46_01735 at 375.470 kb on - strand at 375.499 kb on + strand at 375.504 kb on + strand at 375.525 kb on + strand at 375.535 kb on - strand at 375.632 kb on + strand at 375.639 kb on + strand at 375.720 kb on + strand at 375.721 kb on - strand at 375.763 kb on - strand at 375.824 kb on - strand at 375.856 kb on - strand, within IAI46_01740 at 375.856 kb on - strand, within IAI46_01740 at 375.856 kb on - strand, within IAI46_01740 at 375.857 kb on + strand, within IAI46_01740 at 375.858 kb on - strand, within IAI46_01740 at 375.903 kb on + strand, within IAI46_01740 at 375.904 kb on - strand, within IAI46_01740 at 375.947 kb on + strand, within IAI46_01740 at 375.948 kb on - strand, within IAI46_01740 at 375.948 kb on - strand, within IAI46_01740 at 375.948 kb on - strand, within IAI46_01740 at 375.948 kb on - strand, within IAI46_01740 at 375.951 kb on + strand, within IAI46_01740 at 375.951 kb on + strand, within IAI46_01740 at 375.952 kb on - strand, within IAI46_01740 at 375.952 kb on - strand, within IAI46_01740 at 375.952 kb on - strand, within IAI46_01740 at 375.978 kb on + strand, within IAI46_01740 at 375.978 kb on + strand, within IAI46_01740 at 375.979 kb on - strand, within IAI46_01740 at 375.979 kb on - strand, within IAI46_01740 at 376.018 kb on - strand, within IAI46_01740 at 376.092 kb on + strand, within IAI46_01740 at 376.093 kb on - strand, within IAI46_01740 at 376.093 kb on - strand, within IAI46_01740 at 376.117 kb on - strand, within IAI46_01740 at 376.118 kb on + strand, within IAI46_01740 at 376.119 kb on - strand, within IAI46_01740 at 376.119 kb on - strand, within IAI46_01740 at 376.237 kb on + strand, within IAI46_01740 at 376.238 kb on - strand, within IAI46_01740 at 376.240 kb on + strand, within IAI46_01740 at 376.260 kb on - strand, within IAI46_01740 at 376.265 kb on + strand, within IAI46_01740 at 376.265 kb on + strand, within IAI46_01740 at 376.266 kb on - strand, within IAI46_01740 at 376.266 kb on - strand, within IAI46_01740 at 376.409 kb on + strand, within IAI46_01740 at 376.409 kb on + strand, within IAI46_01740 at 376.410 kb on - strand, within IAI46_01740 at 376.410 kb on - strand, within IAI46_01740 at 376.421 kb on + strand, within IAI46_01740 at 376.421 kb on + strand, within IAI46_01740 at 376.421 kb on + strand, within IAI46_01740 at 376.438 kb on - strand, within IAI46_01740 at 376.482 kb on - strand, within IAI46_01740 at 376.487 kb on + strand, within IAI46_01740 at 376.487 kb on + strand, within IAI46_01740 at 376.487 kb on + strand, within IAI46_01740 at 376.487 kb on + strand, within IAI46_01740 at 376.487 kb on + strand, within IAI46_01740 at 376.488 kb on - strand, within IAI46_01740 at 376.488 kb on - strand, within IAI46_01740 at 376.488 kb on - strand, within IAI46_01740 at 376.518 kb on + strand, within IAI46_01740 at 376.542 kb on + strand, within IAI46_01740 at 376.542 kb on + strand, within IAI46_01740 at 376.543 kb on - strand, within IAI46_01740 at 376.613 kb on + strand, within IAI46_01740 at 376.613 kb on + strand, within IAI46_01740 at 376.613 kb on + strand, within IAI46_01740 at 376.614 kb on - strand, within IAI46_01740 at 376.729 kb on + strand, within IAI46_01740 at 376.730 kb on - strand, within IAI46_01740 at 376.770 kb on + strand, within IAI46_01740 at 376.850 kb on + strand, within IAI46_01740 at 376.850 kb on + strand, within IAI46_01740 at 376.851 kb on - strand, within IAI46_01740 at 376.853 kb on + strand, within IAI46_01740 at 376.853 kb on + strand, within IAI46_01740 at 376.854 kb on - strand, within IAI46_01740 at 376.880 kb on + strand, within IAI46_01740 at 376.881 kb on - strand, within IAI46_01740 at 376.938 kb on + strand, within IAI46_01740 at 376.939 kb on - strand, within IAI46_01740 at 376.955 kb on + strand, within IAI46_01740 at 376.956 kb on - strand, within IAI46_01740 at 376.989 kb on + strand, within IAI46_01740 at 376.989 kb on + strand, within IAI46_01740 at 377.052 kb on - strand, within IAI46_01740 at 377.052 kb on - strand, within IAI46_01740 at 377.063 kb on - strand, within IAI46_01740 at 377.063 kb on - strand, within IAI46_01740 at 377.067 kb on + strand, within IAI46_01740 at 377.067 kb on + strand, within IAI46_01740 at 377.068 kb on - strand, within IAI46_01740 at 377.068 kb on - strand, within IAI46_01740 at 377.068 kb on - strand, within IAI46_01740 at 377.068 kb on - strand, within IAI46_01740 at 377.109 kb on + strand, within IAI46_01740 at 377.114 kb on + strand, within IAI46_01740 at 377.115 kb on - strand, within IAI46_01740 at 377.115 kb on - strand, within IAI46_01740 at 377.166 kb on + strand, within IAI46_01740 at 377.167 kb on - strand, within IAI46_01740 at 377.167 kb on - strand, within IAI46_01740 at 377.169 kb on - strand, within IAI46_01740 at 377.205 kb on - strand, within IAI46_01740 at 377.252 kb on + strand, within IAI46_01740 at 377.274 kb on + strand, within IAI46_01740 at 377.275 kb on - strand, within IAI46_01740 at 377.275 kb on - strand, within IAI46_01740 at 377.275 kb on - strand, within IAI46_01740 at 377.276 kb on + strand, within IAI46_01740 at 377.276 kb on + strand, within IAI46_01740 at 377.277 kb on - strand, within IAI46_01740 at 377.277 kb on - strand, within IAI46_01740 at 377.319 kb on + strand, within IAI46_01740 at 377.319 kb on + strand, within IAI46_01740 at 377.320 kb on - strand, within IAI46_01740 at 377.320 kb on - strand, within IAI46_01740 at 377.321 kb on + strand, within IAI46_01740 at 377.321 kb on + strand, within IAI46_01740 at 377.321 kb on + strand, within IAI46_01740 at 377.321 kb on + strand, within IAI46_01740 at 377.321 kb on + strand, within IAI46_01740 at 377.321 kb on + strand, within IAI46_01740 at 377.321 kb on + strand, within IAI46_01740 at 377.322 kb on - strand, within IAI46_01740 at 377.368 kb on + strand, within IAI46_01740 at 377.369 kb on - strand, within IAI46_01740 at 377.369 kb on - strand, within IAI46_01740 at 377.445 kb on + strand at 377.453 kb on + strand at 377.454 kb on - strand at 377.454 kb on - strand at 377.616 kb on + strand at 377.633 kb on + strand at 377.634 kb on - strand at 377.638 kb on - strand at 377.638 kb on - strand at 377.744 kb on + strand at 377.745 kb on - strand at 377.752 kb on + strand at 377.753 kb on - strand at 377.761 kb on + strand at 377.761 kb on + strand at 377.780 kb on + strand at 377.781 kb on - strand at 377.788 kb on + strand at 377.788 kb on + strand at 377.788 kb on + strand at 377.789 kb on - strand at 377.789 kb on - strand at 378.210 kb on - strand, within IAI46_01745 at 378.410 kb on - strand, within IAI46_01745 at 378.528 kb on - strand at 378.528 kb on - strand at 378.529 kb on + strand at 378.530 kb on - strand at 378.549 kb on + strand at 378.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 374,701 + IAI46_01730 0.84 -0.5 374,701 + IAI46_01730 0.84 -0.0 374,701 + IAI46_01730 0.84 +0.4 374,701 + IAI46_01730 0.84 -0.3 374,701 + IAI46_01730 0.84 +1.3 374,701 + IAI46_01730 0.84 +0.7 374,702 - IAI46_01730 0.84 -0.9 374,702 - IAI46_01730 0.84 -0.5 374,702 - IAI46_01730 0.84 -0.8 374,702 - IAI46_01730 0.84 +0.6 374,703 + IAI46_01730 0.84 +1.1 374,704 - IAI46_01730 0.84 +1.9 374,848 - +0.2 374,917 + IAI46_01735 0.11 +0.5 374,918 - IAI46_01735 0.11 -0.6 374,937 + IAI46_01735 0.14 +0.1 374,937 + IAI46_01735 0.14 -0.6 374,937 + IAI46_01735 0.14 -0.5 374,937 + IAI46_01735 0.14 +0.0 374,937 + IAI46_01735 0.14 -0.4 374,938 - IAI46_01735 0.14 +0.1 374,954 - IAI46_01735 0.16 +2.3 375,274 + IAI46_01735 0.64 +0.4 375,274 + IAI46_01735 0.64 +1.4 375,274 + IAI46_01735 0.64 +0.5 375,274 + IAI46_01735 0.64 +0.5 375,275 - IAI46_01735 0.64 +0.8 375,277 - IAI46_01735 0.65 -0.9 375,277 - IAI46_01735 0.65 -1.0 375,277 - IAI46_01735 0.65 +0.3 375,277 - IAI46_01735 0.65 +0.2 375,305 - IAI46_01735 0.69 +1.2 375,305 - IAI46_01735 0.69 +0.1 375,316 - IAI46_01735 0.71 -0.3 375,316 - IAI46_01735 0.71 +0.4 375,316 - IAI46_01735 0.71 +0.1 375,316 - IAI46_01735 0.71 -1.1 375,382 + IAI46_01735 0.80 -1.3 375,470 - +0.2 375,499 + +0.5 375,504 + -0.1 375,525 + -0.0 375,535 - +0.2 375,632 + +0.4 375,639 + +0.3 375,720 + -0.4 375,721 - -0.2 375,763 - -0.1 375,824 - +0.1 375,856 - IAI46_01740 0.12 +0.6 375,856 - IAI46_01740 0.12 +0.2 375,856 - IAI46_01740 0.12 -1.0 375,857 + IAI46_01740 0.12 +0.4 375,858 - IAI46_01740 0.12 +0.5 375,903 + IAI46_01740 0.14 +0.3 375,904 - IAI46_01740 0.14 +0.6 375,947 + IAI46_01740 0.16 -1.3 375,948 - IAI46_01740 0.16 +0.2 375,948 - IAI46_01740 0.16 -0.2 375,948 - IAI46_01740 0.16 +0.2 375,948 - IAI46_01740 0.16 +0.1 375,951 + IAI46_01740 0.16 +1.0 375,951 + IAI46_01740 0.16 +0.9 375,952 - IAI46_01740 0.16 -0.1 375,952 - IAI46_01740 0.16 -0.1 375,952 - IAI46_01740 0.16 +0.3 375,978 + IAI46_01740 0.18 +0.5 375,978 + IAI46_01740 0.18 -0.4 375,979 - IAI46_01740 0.18 +0.9 375,979 - IAI46_01740 0.18 +1.8 376,018 - IAI46_01740 0.20 -0.4 376,092 + IAI46_01740 0.24 +0.0 376,093 - IAI46_01740 0.24 -0.3 376,093 - IAI46_01740 0.24 +0.2 376,117 - IAI46_01740 0.25 -1.2 376,118 + IAI46_01740 0.25 +1.2 376,119 - IAI46_01740 0.25 +1.1 376,119 - IAI46_01740 0.25 +0.0 376,237 + IAI46_01740 0.31 +0.6 376,238 - IAI46_01740 0.31 +0.9 376,240 + IAI46_01740 0.31 +0.6 376,260 - IAI46_01740 0.32 +0.8 376,265 + IAI46_01740 0.32 +0.2 376,265 + IAI46_01740 0.32 +0.0 376,266 - IAI46_01740 0.33 +1.0 376,266 - IAI46_01740 0.33 -1.1 376,409 + IAI46_01740 0.40 +0.0 376,409 + IAI46_01740 0.40 -1.3 376,410 - IAI46_01740 0.40 +0.6 376,410 - IAI46_01740 0.40 +0.1 376,421 + IAI46_01740 0.40 -1.9 376,421 + IAI46_01740 0.40 -0.4 376,421 + IAI46_01740 0.40 -1.1 376,438 - IAI46_01740 0.41 -0.5 376,482 - IAI46_01740 0.44 +0.7 376,487 + IAI46_01740 0.44 -0.6 376,487 + IAI46_01740 0.44 +0.4 376,487 + IAI46_01740 0.44 +0.3 376,487 + IAI46_01740 0.44 -0.3 376,487 + IAI46_01740 0.44 +0.2 376,488 - IAI46_01740 0.44 +0.6 376,488 - IAI46_01740 0.44 +0.2 376,488 - IAI46_01740 0.44 +0.5 376,518 + IAI46_01740 0.45 +0.4 376,542 + IAI46_01740 0.47 +0.3 376,542 + IAI46_01740 0.47 -0.7 376,543 - IAI46_01740 0.47 -0.8 376,613 + IAI46_01740 0.50 +0.4 376,613 + IAI46_01740 0.50 +0.4 376,613 + IAI46_01740 0.50 +0.1 376,614 - IAI46_01740 0.50 +0.2 376,729 + IAI46_01740 0.56 +0.6 376,730 - IAI46_01740 0.56 +0.0 376,770 + IAI46_01740 0.58 +1.0 376,850 + IAI46_01740 0.62 +0.9 376,850 + IAI46_01740 0.62 +0.8 376,851 - IAI46_01740 0.62 +1.1 376,853 + IAI46_01740 0.63 -0.2 376,853 + IAI46_01740 0.63 +0.3 376,854 - IAI46_01740 0.63 +0.2 376,880 + IAI46_01740 0.64 -0.8 376,881 - IAI46_01740 0.64 +0.5 376,938 + IAI46_01740 0.67 +0.8 376,939 - IAI46_01740 0.67 +0.6 376,955 + IAI46_01740 0.68 -0.5 376,956 - IAI46_01740 0.68 -0.5 376,989 + IAI46_01740 0.70 +1.2 376,989 + IAI46_01740 0.70 +1.4 377,052 - IAI46_01740 0.73 -0.4 377,052 - IAI46_01740 0.73 +0.3 377,063 - IAI46_01740 0.73 +0.3 377,063 - IAI46_01740 0.73 +0.4 377,067 + IAI46_01740 0.74 -0.1 377,067 + IAI46_01740 0.74 -0.6 377,068 - IAI46_01740 0.74 +0.6 377,068 - IAI46_01740 0.74 +1.1 377,068 - IAI46_01740 0.74 -0.7 377,068 - IAI46_01740 0.74 -0.3 377,109 + IAI46_01740 0.76 -1.2 377,114 + IAI46_01740 0.76 +1.1 377,115 - IAI46_01740 0.76 +0.0 377,115 - IAI46_01740 0.76 +1.6 377,166 + IAI46_01740 0.79 +1.8 377,167 - IAI46_01740 0.79 -0.4 377,167 - IAI46_01740 0.79 +0.8 377,169 - IAI46_01740 0.79 -0.8 377,205 - IAI46_01740 0.81 +0.3 377,252 + IAI46_01740 0.83 -0.6 377,274 + IAI46_01740 0.84 +0.9 377,275 - IAI46_01740 0.84 -0.5 377,275 - IAI46_01740 0.84 -0.7 377,275 - IAI46_01740 0.84 +0.8 377,276 + IAI46_01740 0.84 -0.8 377,276 + IAI46_01740 0.84 -0.3 377,277 - IAI46_01740 0.84 +0.4 377,277 - IAI46_01740 0.84 +0.3 377,319 + IAI46_01740 0.86 -0.4 377,319 + IAI46_01740 0.86 -0.2 377,320 - IAI46_01740 0.86 +0.8 377,320 - IAI46_01740 0.86 +1.1 377,321 + IAI46_01740 0.87 +0.0 377,321 + IAI46_01740 0.87 -1.0 377,321 + IAI46_01740 0.87 +0.7 377,321 + IAI46_01740 0.87 +0.2 377,321 + IAI46_01740 0.87 -0.1 377,321 + IAI46_01740 0.87 -0.5 377,321 + IAI46_01740 0.87 -0.3 377,322 - IAI46_01740 0.87 -0.5 377,368 + IAI46_01740 0.89 +1.5 377,369 - IAI46_01740 0.89 -0.4 377,369 - IAI46_01740 0.89 +0.7 377,445 + -0.3 377,453 + +0.4 377,454 - -0.2 377,454 - +0.2 377,616 + +0.7 377,633 + -0.4 377,634 - +0.8 377,638 - -0.3 377,638 - +0.9 377,744 + +0.6 377,745 - -0.7 377,752 + +0.2 377,753 - +0.2 377,761 + +0.3 377,761 + -0.4 377,780 + +0.1 377,781 - +0.5 377,788 + +0.2 377,788 + +0.6 377,788 + -0.3 377,789 - -1.5 377,789 - -0.8 378,210 - IAI46_01745 0.50 -0.3 378,410 - IAI46_01745 0.77 -0.6 378,528 - -1.3 378,528 - -1.0 378,529 + -0.7 378,530 - -0.4 378,549 + -0.7 378,579 - -0.1
Or see this region's nucleotide sequence