Experiment: an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13015 and AMB_RS13020 are separated by 54 nucleotides AMB_RS13020 and AMB_RS13025 overlap by 1 nucleotides AMB_RS13025 and AMB_RS13030 are separated by 57 nucleotides AMB_RS13030 and AMB_RS13035 are separated by 38 nucleotides
AMB_RS13015: AMB_RS13015 - TetR/AcrR family transcriptional regulator, at 2,772,585 to 2,773,262
_RS13015
AMB_RS13020: AMB_RS13020 - KR domain-containing protein, at 2,773,317 to 2,774,087
_RS13020
AMB_RS13025: AMB_RS13025 - acyl-CoA dehydrogenase, at 2,774,087 to 2,775,271
_RS13025
AMB_RS13030: AMB_RS13030 - enoyl-CoA hydratase, at 2,775,329 to 2,776,147
_RS13030
AMB_RS13035: AMB_RS13035 - NAD(P)-dependent oxidoreductase, at 2,776,186 to 2,776,932
_RS13035
Position (kb)
2774
2775
2776 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2773.138 kb on + strand, within AMB_RS13015 at 2773.139 kb on - strand, within AMB_RS13015 at 2773.273 kb on - strand at 2773.297 kb on + strand at 2773.298 kb on - strand at 2773.299 kb on + strand at 2773.300 kb on - strand at 2773.300 kb on - strand at 2773.318 kb on + strand at 2773.319 kb on - strand at 2773.319 kb on - strand at 2773.319 kb on - strand at 2773.496 kb on + strand, within AMB_RS13020 at 2773.497 kb on - strand, within AMB_RS13020 at 2773.533 kb on - strand, within AMB_RS13020 at 2773.534 kb on + strand, within AMB_RS13020 at 2773.535 kb on - strand, within AMB_RS13020 at 2773.535 kb on - strand, within AMB_RS13020 at 2773.535 kb on - strand, within AMB_RS13020 at 2773.643 kb on - strand, within AMB_RS13020 at 2773.643 kb on - strand, within AMB_RS13020 at 2774.090 kb on + strand at 2774.091 kb on - strand at 2774.106 kb on + strand at 2774.106 kb on + strand at 2774.106 kb on + strand at 2774.107 kb on - strand at 2774.107 kb on - strand at 2774.107 kb on - strand at 2774.107 kb on - strand at 2774.107 kb on - strand at 2774.107 kb on - strand at 2774.107 kb on - strand at 2774.107 kb on - strand at 2774.154 kb on + strand at 2774.155 kb on - strand at 2774.155 kb on - strand at 2774.155 kb on - strand at 2774.155 kb on - strand at 2774.155 kb on - strand at 2774.343 kb on + strand, within AMB_RS13025 at 2774.343 kb on + strand, within AMB_RS13025 at 2774.739 kb on + strand, within AMB_RS13025 at 2774.740 kb on - strand, within AMB_RS13025 at 2774.740 kb on - strand, within AMB_RS13025 at 2774.793 kb on + strand, within AMB_RS13025 at 2774.793 kb on + strand, within AMB_RS13025 at 2774.793 kb on + strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.794 kb on - strand, within AMB_RS13025 at 2774.907 kb on + strand, within AMB_RS13025 at 2774.965 kb on - strand, within AMB_RS13025 at 2775.085 kb on + strand, within AMB_RS13025 at 2775.275 kb on - strand at 2775.375 kb on + strand at 2775.375 kb on + strand at 2775.375 kb on + strand at 2775.375 kb on + strand at 2775.375 kb on + strand at 2775.375 kb on + strand at 2775.375 kb on + strand at 2775.376 kb on - strand at 2775.376 kb on - strand at 2775.376 kb on - strand at 2775.376 kb on - strand at 2775.376 kb on - strand at 2775.376 kb on - strand at 2775.576 kb on + strand, within AMB_RS13030 at 2775.576 kb on + strand, within AMB_RS13030 at 2775.576 kb on + strand, within AMB_RS13030 at 2775.576 kb on + strand, within AMB_RS13030 at 2775.576 kb on + strand, within AMB_RS13030 at 2775.577 kb on - strand, within AMB_RS13030 at 2775.577 kb on - strand, within AMB_RS13030 at 2775.577 kb on - strand, within AMB_RS13030 at 2775.577 kb on - strand, within AMB_RS13030 at 2775.577 kb on - strand, within AMB_RS13030 at 2775.577 kb on - strand, within AMB_RS13030 at 2775.577 kb on - strand, within AMB_RS13030 at 2775.579 kb on + strand, within AMB_RS13030 at 2775.579 kb on + strand, within AMB_RS13030 at 2775.624 kb on + strand, within AMB_RS13030 at 2775.624 kb on + strand, within AMB_RS13030 at 2775.625 kb on - strand, within AMB_RS13030 at 2776.123 kb on - strand at 2776.187 kb on + strand at 2776.187 kb on + strand at 2776.187 kb on + strand at 2776.188 kb on - strand at 2776.239 kb on - strand at 2776.239 kb on - strand at 2776.239 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction an_mag_3 remove 2,773,138 + AMB_RS13015 0.82 -1.4 2,773,139 - AMB_RS13015 0.82 -0.8 2,773,273 - -0.8 2,773,297 + +0.2 2,773,298 - +0.2 2,773,299 + -1.4 2,773,300 - -0.8 2,773,300 - +0.2 2,773,318 + -0.6 2,773,319 - -1.4 2,773,319 - -1.4 2,773,319 - -2.6 2,773,496 + AMB_RS13020 0.23 +0.2 2,773,497 - AMB_RS13020 0.23 -2.1 2,773,533 - AMB_RS13020 0.28 -2.4 2,773,534 + AMB_RS13020 0.28 -1.8 2,773,535 - AMB_RS13020 0.28 -1.8 2,773,535 - AMB_RS13020 0.28 -2.1 2,773,535 - AMB_RS13020 0.28 -1.8 2,773,643 - AMB_RS13020 0.42 -2.6 2,773,643 - AMB_RS13020 0.42 -1.8 2,774,090 + -1.8 2,774,091 - +0.2 2,774,106 + -0.8 2,774,106 + -3.1 2,774,106 + -1.8 2,774,107 - -0.8 2,774,107 - -0.3 2,774,107 - -1.4 2,774,107 - +0.2 2,774,107 - +0.2 2,774,107 - -0.8 2,774,107 - -0.7 2,774,107 - -0.4 2,774,154 + -0.8 2,774,155 - -2.6 2,774,155 - -1.4 2,774,155 - -0.2 2,774,155 - -4.4 2,774,155 - -4.1 2,774,343 + AMB_RS13025 0.22 +0.2 2,774,343 + AMB_RS13025 0.22 -1.8 2,774,739 + AMB_RS13025 0.55 +0.2 2,774,740 - AMB_RS13025 0.55 -2.4 2,774,740 - AMB_RS13025 0.55 +0.8 2,774,793 + AMB_RS13025 0.60 -2.8 2,774,793 + AMB_RS13025 0.60 -0.8 2,774,793 + AMB_RS13025 0.60 -0.8 2,774,794 - AMB_RS13025 0.60 -1.4 2,774,794 - AMB_RS13025 0.60 +0.2 2,774,794 - AMB_RS13025 0.60 -0.2 2,774,794 - AMB_RS13025 0.60 -1.6 2,774,794 - AMB_RS13025 0.60 -0.8 2,774,794 - AMB_RS13025 0.60 -0.8 2,774,794 - AMB_RS13025 0.60 -3.4 2,774,794 - AMB_RS13025 0.60 -2.8 2,774,794 - AMB_RS13025 0.60 +0.2 2,774,907 + AMB_RS13025 0.69 -2.6 2,774,965 - AMB_RS13025 0.74 +0.4 2,775,085 + AMB_RS13025 0.84 -2.1 2,775,275 - +0.2 2,775,375 + +0.2 2,775,375 + +0.2 2,775,375 + -1.3 2,775,375 + -0.8 2,775,375 + -1.2 2,775,375 + +0.2 2,775,375 + +0.2 2,775,376 - +0.2 2,775,376 - +0.2 2,775,376 - -3.1 2,775,376 - -3.3 2,775,376 - -2.3 2,775,376 - -1.3 2,775,576 + AMB_RS13030 0.30 -1.8 2,775,576 + AMB_RS13030 0.30 +0.2 2,775,576 + AMB_RS13030 0.30 -2.6 2,775,576 + AMB_RS13030 0.30 -0.8 2,775,576 + AMB_RS13030 0.30 +0.2 2,775,577 - AMB_RS13030 0.30 +0.2 2,775,577 - AMB_RS13030 0.30 +0.2 2,775,577 - AMB_RS13030 0.30 -0.0 2,775,577 - AMB_RS13030 0.30 +0.2 2,775,577 - AMB_RS13030 0.30 -0.7 2,775,577 - AMB_RS13030 0.30 -2.1 2,775,577 - AMB_RS13030 0.30 -0.4 2,775,579 + AMB_RS13030 0.31 -3.7 2,775,579 + AMB_RS13030 0.31 -0.8 2,775,624 + AMB_RS13030 0.36 -1.8 2,775,624 + AMB_RS13030 0.36 -1.1 2,775,625 - AMB_RS13030 0.36 +0.2 2,776,123 - +0.2 2,776,187 + -0.8 2,776,187 + -0.7 2,776,187 + -0.4 2,776,188 - -0.8 2,776,239 - -2.6 2,776,239 - -1.3 2,776,239 - -0.8
Or see this region's nucleotide sequence