Experiment: an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12680 and AMB_RS12685 are separated by 14 nucleotides AMB_RS12685 and AMB_RS12690 are separated by 10 nucleotides AMB_RS12690 and AMB_RS12695 are separated by 6 nucleotides AMB_RS12695 and AMB_RS12700 are separated by 90 nucleotides AMB_RS12700 and AMB_RS12705 are separated by 30 nucleotides
AMB_RS12680: AMB_RS12680 - serine--tRNA ligase, at 2,702,881 to 2,704,149
_RS12680
AMB_RS12685: AMB_RS12685 - twin-arginine translocase subunit TatC, at 2,704,164 to 2,704,988
_RS12685
AMB_RS12690: AMB_RS12690 - twin-arginine translocase subunit TatB, at 2,704,999 to 2,705,370
_RS12690
AMB_RS12695: AMB_RS12695 - twin-arginine translocase TatA/TatE family subunit, at 2,705,377 to 2,705,616
_RS12695
AMB_RS12700: AMB_RS12700 - DUF454 domain-containing protein, at 2,705,707 to 2,706,060
_RS12700
AMB_RS12705: AMB_RS12705 - SMC-Scp complex subunit ScpB, at 2,706,091 to 2,706,741
_RS12705
Position (kb)
2704
2705
2706 Strain fitness (log2 ratio)
-2
-1
0
1 at 2705.760 kb on + strand, within AMB_RS12700 at 2705.761 kb on - strand, within AMB_RS12700 at 2705.819 kb on + strand, within AMB_RS12700 at 2705.819 kb on + strand, within AMB_RS12700 at 2705.820 kb on - strand, within AMB_RS12700 at 2705.924 kb on + strand, within AMB_RS12700 at 2706.092 kb on + strand at 2706.093 kb on - strand at 2706.174 kb on + strand, within AMB_RS12705
Per-strain Table
Position Strand Gene LocusTag Fraction an_mag_3 remove 2,705,760 + AMB_RS12700 0.15 +0.2 2,705,761 - AMB_RS12700 0.15 -0.3 2,705,819 + AMB_RS12700 0.32 -0.1 2,705,819 + AMB_RS12700 0.32 -0.7 2,705,820 - AMB_RS12700 0.32 -1.4 2,705,924 + AMB_RS12700 0.61 -2.1 2,706,092 + -2.6 2,706,093 - -1.0 2,706,174 + AMB_RS12705 0.13 +0.2
Or see this region's nucleotide sequence