Experiment: an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01955 and AMB_RS01960 overlap by 4 nucleotides AMB_RS01960 and AMB_RS01965 are separated by 6 nucleotides AMB_RS01965 and AMB_RS01970 are separated by 10 nucleotides AMB_RS01970 and AMB_RS01975 are separated by 4 nucleotides AMB_RS01975 and AMB_RS01980 are separated by 2 nucleotides
AMB_RS01955: AMB_RS01955 - replication protein, at 412,712 to 415,702
_RS01955
AMB_RS01960: AMB_RS01960 - hypothetical protein, at 415,699 to 416,196
_RS01960
AMB_RS01965: AMB_RS01965 - hypothetical protein, at 416,203 to 417,072
_RS01965
AMB_RS01970: AMB_RS01970 - hypothetical protein, at 417,083 to 417,493
_RS01970
AMB_RS01975: AMB_RS01975 - hypothetical protein, at 417,498 to 418,064
_RS01975
AMB_RS01980: AMB_RS01980 - hypothetical protein, at 418,067 to 419,410
_RS01980
Position (kb) 416
417
418 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 415.331 kb on + strand, within AMB_RS01955 at 415.331 kb on + strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.332 kb on - strand, within AMB_RS01955 at 415.447 kb on + strand at 415.694 kb on + strand at 415.745 kb on - strand at 415.745 kb on - strand at 415.753 kb on + strand, within AMB_RS01960 at 416.008 kb on + strand, within AMB_RS01960 at 416.008 kb on + strand, within AMB_RS01960 at 416.009 kb on - strand, within AMB_RS01960 at 416.155 kb on - strand at 416.155 kb on - strand at 416.155 kb on - strand at 416.155 kb on - strand at 416.350 kb on - strand, within AMB_RS01965 at 416.478 kb on + strand, within AMB_RS01965 at 416.479 kb on - strand, within AMB_RS01965 at 417.043 kb on + strand at 417.044 kb on - strand at 417.101 kb on + strand at 417.243 kb on - strand, within AMB_RS01970 at 417.243 kb on - strand, within AMB_RS01970 at 417.288 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.301 kb on - strand, within AMB_RS01970 at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.480 kb on - strand at 417.792 kb on + strand, within AMB_RS01975 at 417.792 kb on + strand, within AMB_RS01975 at 417.792 kb on + strand, within AMB_RS01975 at 417.876 kb on + strand, within AMB_RS01975 at 417.876 kb on + strand, within AMB_RS01975 at 417.902 kb on + strand, within AMB_RS01975 at 417.903 kb on - strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.939 kb on + strand, within AMB_RS01975 at 417.940 kb on - strand, within AMB_RS01975 at 417.940 kb on - strand, within AMB_RS01975 at 417.940 kb on - strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 417.999 kb on + strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.000 kb on - strand, within AMB_RS01975 at 418.012 kb on - strand at 418.023 kb on + strand at 418.023 kb on + strand at 418.023 kb on + strand at 418.025 kb on + strand at 418.052 kb on + strand at 418.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction an_mag_3 remove 415,331 + AMB_RS01955 0.88 -1.2 415,331 + AMB_RS01955 0.88 -1.2 415,332 - AMB_RS01955 0.88 -2.0 415,332 - AMB_RS01955 0.88 -1.2 415,332 - AMB_RS01955 0.88 -1.1 415,332 - AMB_RS01955 0.88 -1.0 415,332 - AMB_RS01955 0.88 +0.4 415,332 - AMB_RS01955 0.88 -1.6 415,332 - AMB_RS01955 0.88 -0.6 415,332 - AMB_RS01955 0.88 -0.2 415,447 + -0.1 415,694 + +0.4 415,745 - -0.5 415,745 - -1.1 415,753 + AMB_RS01960 0.11 -1.2 416,008 + AMB_RS01960 0.62 -3.6 416,008 + AMB_RS01960 0.62 -2.8 416,009 - AMB_RS01960 0.62 -1.2 416,155 - -0.6 416,155 - -1.5 416,155 - -0.6 416,155 - -0.3 416,350 - AMB_RS01965 0.17 -1.6 416,478 + AMB_RS01965 0.32 +0.4 416,479 - AMB_RS01965 0.32 -0.6 417,043 + -1.2 417,044 - -0.3 417,101 + +0.4 417,243 - AMB_RS01970 0.39 -1.2 417,243 - AMB_RS01970 0.39 -0.6 417,288 - AMB_RS01970 0.50 -1.2 417,301 - AMB_RS01970 0.53 +0.4 417,301 - AMB_RS01970 0.53 +0.4 417,301 - AMB_RS01970 0.53 +0.4 417,301 - AMB_RS01970 0.53 -1.6 417,480 - -0.6 417,480 - -0.6 417,480 - -0.6 417,480 - -2.2 417,480 - -1.6 417,480 - -0.0 417,792 + AMB_RS01975 0.52 +0.4 417,792 + AMB_RS01975 0.52 -2.2 417,792 + AMB_RS01975 0.52 -0.1 417,876 + AMB_RS01975 0.67 -1.2 417,876 + AMB_RS01975 0.67 -0.3 417,902 + AMB_RS01975 0.71 +0.4 417,903 - AMB_RS01975 0.71 +0.4 417,939 + AMB_RS01975 0.78 -3.2 417,939 + AMB_RS01975 0.78 -4.0 417,939 + AMB_RS01975 0.78 +0.4 417,939 + AMB_RS01975 0.78 -0.6 417,940 - AMB_RS01975 0.78 -0.6 417,940 - AMB_RS01975 0.78 -1.2 417,940 - AMB_RS01975 0.78 -1.4 417,999 + AMB_RS01975 0.88 +0.4 417,999 + AMB_RS01975 0.88 -0.5 417,999 + AMB_RS01975 0.88 -0.6 417,999 + AMB_RS01975 0.88 -0.6 417,999 + AMB_RS01975 0.88 +1.4 418,000 - AMB_RS01975 0.89 +0.0 418,000 - AMB_RS01975 0.89 +0.4 418,000 - AMB_RS01975 0.89 -1.2 418,000 - AMB_RS01975 0.89 -2.8 418,012 - +0.4 418,023 + -1.2 418,023 + -2.1 418,023 + -2.6 418,025 + +0.4 418,052 + +0.4 418,053 - -0.2
Or see this region's nucleotide sequence