Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS06285 and ABIE40_RS06290 are separated by 133 nucleotides ABIE40_RS06290 and ABIE40_RS06295 are separated by 3 nucleotides ABIE40_RS06295 and ABIE40_RS06300 are separated by 94 nucleotides ABIE40_RS06300 and ABIE40_RS06305 overlap by 4 nucleotides ABIE40_RS06305 and ABIE40_RS06310 are separated by 64 nucleotides
ABIE40_RS06285: ABIE40_RS06285 - PDZ domain-containing protein, at 1,231,564 to 1,232,253
_RS06285
ABIE40_RS06290: ABIE40_RS06290 - LysE family translocator, at 1,232,387 to 1,233,022
_RS06290
ABIE40_RS06295: ABIE40_RS06295 - alanine racemase, at 1,233,026 to 1,234,198
_RS06295
ABIE40_RS06300: ABIE40_RS06300 - transcriptional regulator, at 1,234,293 to 1,234,655
_RS06300
ABIE40_RS06305: ABIE40_RS06305 - DUF6516 family protein, at 1,234,652 to 1,234,930
_RS06305
ABIE40_RS06310: ABIE40_RS06310 - replicative DNA helicase, at 1,234,995 to 1,236,488
_RS06310
Position (kb)
1233
1234
1235 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.065 kb on + strand, within ABIE40_RS06285 at 1232.102 kb on - strand, within ABIE40_RS06285 at 1232.199 kb on + strand at 1232.199 kb on + strand at 1232.200 kb on - strand at 1232.252 kb on - strand at 1232.334 kb on + strand at 1232.367 kb on + strand at 1232.368 kb on - strand at 1232.368 kb on - strand at 1232.368 kb on - strand at 1232.368 kb on - strand at 1232.485 kb on + strand, within ABIE40_RS06290 at 1232.485 kb on + strand, within ABIE40_RS06290 at 1232.486 kb on - strand, within ABIE40_RS06290 at 1232.486 kb on - strand, within ABIE40_RS06290 at 1232.486 kb on - strand, within ABIE40_RS06290 at 1232.486 kb on - strand, within ABIE40_RS06290 at 1232.556 kb on + strand, within ABIE40_RS06290 at 1232.556 kb on + strand, within ABIE40_RS06290 at 1232.556 kb on + strand, within ABIE40_RS06290 at 1232.556 kb on + strand, within ABIE40_RS06290 at 1232.556 kb on + strand, within ABIE40_RS06290 at 1232.557 kb on - strand, within ABIE40_RS06290 at 1232.557 kb on - strand, within ABIE40_RS06290 at 1232.557 kb on - strand, within ABIE40_RS06290 at 1232.557 kb on - strand, within ABIE40_RS06290 at 1232.557 kb on - strand, within ABIE40_RS06290 at 1232.557 kb on - strand, within ABIE40_RS06290 at 1232.639 kb on + strand, within ABIE40_RS06290 at 1232.639 kb on + strand, within ABIE40_RS06290 at 1232.640 kb on - strand, within ABIE40_RS06290 at 1232.640 kb on - strand, within ABIE40_RS06290 at 1232.733 kb on - strand, within ABIE40_RS06290 at 1233.027 kb on + strand at 1233.027 kb on + strand at 1233.027 kb on + strand at 1233.027 kb on + strand at 1233.027 kb on + strand at 1233.028 kb on - strand at 1233.028 kb on - strand at 1233.028 kb on - strand at 1233.028 kb on - strand at 1233.028 kb on - strand at 1233.028 kb on - strand at 1233.028 kb on - strand at 1233.028 kb on - strand at 1233.045 kb on + strand at 1233.046 kb on - strand at 1233.046 kb on - strand at 1233.046 kb on - strand at 1233.244 kb on - strand, within ABIE40_RS06295 at 1233.244 kb on - strand, within ABIE40_RS06295 at 1233.321 kb on + strand, within ABIE40_RS06295 at 1233.321 kb on + strand, within ABIE40_RS06295 at 1233.321 kb on + strand, within ABIE40_RS06295 at 1233.322 kb on - strand, within ABIE40_RS06295 at 1233.334 kb on - strand, within ABIE40_RS06295 at 1233.390 kb on + strand, within ABIE40_RS06295 at 1233.390 kb on + strand, within ABIE40_RS06295 at 1233.390 kb on + strand, within ABIE40_RS06295 at 1233.498 kb on + strand, within ABIE40_RS06295 at 1233.499 kb on - strand, within ABIE40_RS06295 at 1233.591 kb on + strand, within ABIE40_RS06295 at 1233.592 kb on - strand, within ABIE40_RS06295 at 1233.592 kb on - strand, within ABIE40_RS06295 at 1233.592 kb on - strand, within ABIE40_RS06295 at 1233.740 kb on + strand, within ABIE40_RS06295 at 1233.740 kb on + strand, within ABIE40_RS06295 at 1233.741 kb on - strand, within ABIE40_RS06295 at 1233.741 kb on - strand, within ABIE40_RS06295 at 1233.741 kb on - strand, within ABIE40_RS06295 at 1233.741 kb on - strand, within ABIE40_RS06295 at 1233.741 kb on - strand, within ABIE40_RS06295 at 1233.793 kb on - strand, within ABIE40_RS06295 at 1233.937 kb on - strand, within ABIE40_RS06295 at 1233.997 kb on + strand, within ABIE40_RS06295 at 1233.997 kb on + strand, within ABIE40_RS06295 at 1233.997 kb on + strand, within ABIE40_RS06295 at 1233.999 kb on + strand, within ABIE40_RS06295 at 1233.999 kb on + strand, within ABIE40_RS06295 at 1233.999 kb on + strand, within ABIE40_RS06295 at 1233.999 kb on + strand, within ABIE40_RS06295 at 1233.999 kb on + strand, within ABIE40_RS06295 at 1233.999 kb on + strand, within ABIE40_RS06295 at 1234.000 kb on - strand, within ABIE40_RS06295 at 1234.000 kb on - strand, within ABIE40_RS06295 at 1234.000 kb on - strand, within ABIE40_RS06295 at 1234.000 kb on - strand, within ABIE40_RS06295 at 1234.000 kb on - strand, within ABIE40_RS06295 at 1234.000 kb on - strand, within ABIE40_RS06295 at 1234.000 kb on - strand, within ABIE40_RS06295 at 1234.035 kb on + strand, within ABIE40_RS06295 at 1234.035 kb on + strand, within ABIE40_RS06295 at 1234.035 kb on + strand, within ABIE40_RS06295 at 1234.206 kb on - strand at 1234.235 kb on + strand at 1234.235 kb on + strand at 1234.235 kb on + strand at 1234.236 kb on - strand at 1234.236 kb on - strand at 1234.305 kb on - strand at 1234.673 kb on + strand at 1234.674 kb on - strand at 1234.674 kb on - strand at 1234.674 kb on - strand at 1234.785 kb on + strand, within ABIE40_RS06305 at 1234.810 kb on - strand, within ABIE40_RS06305 at 1234.815 kb on + strand, within ABIE40_RS06305 at 1234.816 kb on - strand, within ABIE40_RS06305 at 1234.816 kb on - strand, within ABIE40_RS06305 at 1234.816 kb on - strand, within ABIE40_RS06305 at 1234.816 kb on - strand, within ABIE40_RS06305 at 1234.820 kb on + strand, within ABIE40_RS06305 at 1234.820 kb on + strand, within ABIE40_RS06305 at 1234.820 kb on + strand, within ABIE40_RS06305 at 1234.820 kb on + strand, within ABIE40_RS06305 at 1234.820 kb on + strand, within ABIE40_RS06305 at 1234.821 kb on - strand, within ABIE40_RS06305 at 1234.821 kb on - strand, within ABIE40_RS06305 at 1234.821 kb on - strand, within ABIE40_RS06305 at 1234.821 kb on - strand, within ABIE40_RS06305 at 1234.827 kb on + strand, within ABIE40_RS06305 at 1234.827 kb on + strand, within ABIE40_RS06305 at 1234.827 kb on + strand, within ABIE40_RS06305 at 1234.827 kb on + strand, within ABIE40_RS06305 at 1234.827 kb on + strand, within ABIE40_RS06305 at 1234.828 kb on - strand, within ABIE40_RS06305 at 1234.828 kb on - strand, within ABIE40_RS06305 at 1234.828 kb on - strand, within ABIE40_RS06305 at 1234.828 kb on - strand, within ABIE40_RS06305 at 1234.828 kb on - strand, within ABIE40_RS06305 at 1234.828 kb on - strand, within ABIE40_RS06305 at 1234.833 kb on + strand, within ABIE40_RS06305 at 1234.833 kb on + strand, within ABIE40_RS06305 at 1234.833 kb on + strand, within ABIE40_RS06305 at 1234.833 kb on + strand, within ABIE40_RS06305 at 1234.834 kb on - strand, within ABIE40_RS06305
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 1,232,065 + ABIE40_RS06285 0.73 +0.4 1,232,065 + ABIE40_RS06285 0.73 -1.3 1,232,065 + ABIE40_RS06285 0.73 +0.3 1,232,065 + ABIE40_RS06285 0.73 -1.1 1,232,065 + ABIE40_RS06285 0.73 +0.3 1,232,065 + ABIE40_RS06285 0.73 -0.4 1,232,065 + ABIE40_RS06285 0.73 -0.3 1,232,065 + ABIE40_RS06285 0.73 +1.2 1,232,102 - ABIE40_RS06285 0.78 +1.3 1,232,199 + +0.5 1,232,199 + +1.3 1,232,200 - +1.0 1,232,252 - +0.5 1,232,334 + +0.5 1,232,367 + -0.0 1,232,368 - +1.5 1,232,368 - +1.0 1,232,368 - -0.8 1,232,368 - -0.4 1,232,485 + ABIE40_RS06290 0.15 -0.0 1,232,485 + ABIE40_RS06290 0.15 +0.0 1,232,486 - ABIE40_RS06290 0.16 +1.1 1,232,486 - ABIE40_RS06290 0.16 -1.5 1,232,486 - ABIE40_RS06290 0.16 -0.7 1,232,486 - ABIE40_RS06290 0.16 +1.9 1,232,556 + ABIE40_RS06290 0.27 +1.9 1,232,556 + ABIE40_RS06290 0.27 +0.2 1,232,556 + ABIE40_RS06290 0.27 -0.5 1,232,556 + ABIE40_RS06290 0.27 +0.8 1,232,556 + ABIE40_RS06290 0.27 +0.8 1,232,557 - ABIE40_RS06290 0.27 +1.5 1,232,557 - ABIE40_RS06290 0.27 -1.2 1,232,557 - ABIE40_RS06290 0.27 -0.0 1,232,557 - ABIE40_RS06290 0.27 -2.1 1,232,557 - ABIE40_RS06290 0.27 -1.1 1,232,557 - ABIE40_RS06290 0.27 +0.5 1,232,639 + ABIE40_RS06290 0.40 -0.7 1,232,639 + ABIE40_RS06290 0.40 -0.5 1,232,640 - ABIE40_RS06290 0.40 +0.6 1,232,640 - ABIE40_RS06290 0.40 -1.1 1,232,733 - ABIE40_RS06290 0.54 +0.0 1,233,027 + -2.9 1,233,027 + -0.3 1,233,027 + -1.6 1,233,027 + -0.1 1,233,027 + -0.1 1,233,028 - +0.1 1,233,028 - -0.5 1,233,028 - +0.7 1,233,028 - +0.2 1,233,028 - +0.2 1,233,028 - +0.7 1,233,028 - -1.0 1,233,028 - +0.8 1,233,045 + +0.7 1,233,046 - +0.2 1,233,046 - -0.4 1,233,046 - +0.2 1,233,244 - ABIE40_RS06295 0.19 -2.1 1,233,244 - ABIE40_RS06295 0.19 -0.5 1,233,321 + ABIE40_RS06295 0.25 -1.5 1,233,321 + ABIE40_RS06295 0.25 +3.0 1,233,321 + ABIE40_RS06295 0.25 +0.1 1,233,322 - ABIE40_RS06295 0.25 -2.4 1,233,334 - ABIE40_RS06295 0.26 -0.5 1,233,390 + ABIE40_RS06295 0.31 +1.2 1,233,390 + ABIE40_RS06295 0.31 +0.4 1,233,390 + ABIE40_RS06295 0.31 -0.7 1,233,498 + ABIE40_RS06295 0.40 +1.2 1,233,499 - ABIE40_RS06295 0.40 -0.1 1,233,591 + ABIE40_RS06295 0.48 +0.7 1,233,592 - ABIE40_RS06295 0.48 +0.8 1,233,592 - ABIE40_RS06295 0.48 +0.3 1,233,592 - ABIE40_RS06295 0.48 -1.3 1,233,740 + ABIE40_RS06295 0.61 +0.4 1,233,740 + ABIE40_RS06295 0.61 -0.8 1,233,741 - ABIE40_RS06295 0.61 -0.1 1,233,741 - ABIE40_RS06295 0.61 +0.3 1,233,741 - ABIE40_RS06295 0.61 +1.0 1,233,741 - ABIE40_RS06295 0.61 -0.0 1,233,741 - ABIE40_RS06295 0.61 +0.5 1,233,793 - ABIE40_RS06295 0.65 -3.1 1,233,937 - ABIE40_RS06295 0.78 -1.4 1,233,997 + ABIE40_RS06295 0.83 +1.0 1,233,997 + ABIE40_RS06295 0.83 -0.4 1,233,997 + ABIE40_RS06295 0.83 +0.6 1,233,999 + ABIE40_RS06295 0.83 +2.7 1,233,999 + ABIE40_RS06295 0.83 +0.0 1,233,999 + ABIE40_RS06295 0.83 +0.4 1,233,999 + ABIE40_RS06295 0.83 +0.2 1,233,999 + ABIE40_RS06295 0.83 +0.5 1,233,999 + ABIE40_RS06295 0.83 -0.4 1,234,000 - ABIE40_RS06295 0.83 -1.0 1,234,000 - ABIE40_RS06295 0.83 +0.6 1,234,000 - ABIE40_RS06295 0.83 -0.9 1,234,000 - ABIE40_RS06295 0.83 -0.3 1,234,000 - ABIE40_RS06295 0.83 +1.0 1,234,000 - ABIE40_RS06295 0.83 +0.0 1,234,000 - ABIE40_RS06295 0.83 -0.3 1,234,035 + ABIE40_RS06295 0.86 +0.2 1,234,035 + ABIE40_RS06295 0.86 -0.6 1,234,035 + ABIE40_RS06295 0.86 -0.9 1,234,206 - +0.2 1,234,235 + +0.7 1,234,235 + +0.6 1,234,235 + -1.0 1,234,236 - -0.5 1,234,236 - +0.2 1,234,305 - -0.6 1,234,673 + -0.7 1,234,674 - +0.6 1,234,674 - +0.3 1,234,674 - +1.5 1,234,785 + ABIE40_RS06305 0.48 +0.6 1,234,810 - ABIE40_RS06305 0.57 +0.7 1,234,815 + ABIE40_RS06305 0.58 -0.0 1,234,816 - ABIE40_RS06305 0.59 -0.5 1,234,816 - ABIE40_RS06305 0.59 -0.4 1,234,816 - ABIE40_RS06305 0.59 +0.6 1,234,816 - ABIE40_RS06305 0.59 -1.1 1,234,820 + ABIE40_RS06305 0.60 +0.4 1,234,820 + ABIE40_RS06305 0.60 -0.6 1,234,820 + ABIE40_RS06305 0.60 +0.4 1,234,820 + ABIE40_RS06305 0.60 -0.0 1,234,820 + ABIE40_RS06305 0.60 -0.3 1,234,821 - ABIE40_RS06305 0.61 -0.4 1,234,821 - ABIE40_RS06305 0.61 +1.3 1,234,821 - ABIE40_RS06305 0.61 -2.0 1,234,821 - ABIE40_RS06305 0.61 -2.7 1,234,827 + ABIE40_RS06305 0.63 -0.3 1,234,827 + ABIE40_RS06305 0.63 +0.3 1,234,827 + ABIE40_RS06305 0.63 -0.2 1,234,827 + ABIE40_RS06305 0.63 +0.3 1,234,827 + ABIE40_RS06305 0.63 -0.0 1,234,828 - ABIE40_RS06305 0.63 -0.0 1,234,828 - ABIE40_RS06305 0.63 -1.7 1,234,828 - ABIE40_RS06305 0.63 -1.5 1,234,828 - ABIE40_RS06305 0.63 -1.6 1,234,828 - ABIE40_RS06305 0.63 +0.9 1,234,828 - ABIE40_RS06305 0.63 +1.9 1,234,833 + ABIE40_RS06305 0.65 +0.9 1,234,833 + ABIE40_RS06305 0.65 -0.7 1,234,833 + ABIE40_RS06305 0.65 -0.2 1,234,833 + ABIE40_RS06305 0.65 -2.1 1,234,834 - ABIE40_RS06305 0.65 +0.6
Or see this region's nucleotide sequence