Experiment: Cadmium acetate 40 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_15965 and IAI46_15970 are separated by 121 nucleotides IAI46_15970 and IAI46_15975 are separated by 47 nucleotides IAI46_15975 and IAI46_15980 are separated by 61 nucleotides IAI46_15980 and IAI46_15985 overlap by 17 nucleotides IAI46_15985 and IAI46_15990 are separated by 210 nucleotides
IAI46_15965: IAI46_15965 - flagellar basal body P-ring formation protein FlgA, at 3,349,587 to 3,350,240
_15965
IAI46_15970: IAI46_15970 - anti-sigma-28 factor FlgM, at 3,350,362 to 3,350,688
_15970
IAI46_15975: IAI46_15975 - flagellar export chaperone FlgN, at 3,350,736 to 3,351,170
_15975
IAI46_15980: IAI46_15980 - translesion error-prone DNA polymerase V subunit UmuC, at 3,351,232 to 3,352,494
_15980
IAI46_15985: IAI46_15985 - translesion error-prone DNA polymerase V autoproteolytic subunit, at 3,352,478 to 3,352,912
_15985
IAI46_15990: IAI46_15990 - flagellar protein FlhE, at 3,353,123 to 3,353,527
_15990
Position (kb)
3351
3352
3353 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3350.318 kb on - strand at 3350.367 kb on + strand at 3350.544 kb on + strand, within IAI46_15970 at 3350.699 kb on - strand at 3350.898 kb on + strand, within IAI46_15975 at 3350.898 kb on + strand, within IAI46_15975 at 3350.899 kb on - strand, within IAI46_15975 at 3350.899 kb on - strand, within IAI46_15975 at 3350.992 kb on + strand, within IAI46_15975 at 3351.032 kb on + strand, within IAI46_15975 at 3351.032 kb on + strand, within IAI46_15975 at 3351.112 kb on - strand, within IAI46_15975 at 3351.189 kb on + strand at 3351.189 kb on + strand at 3351.190 kb on - strand at 3351.190 kb on - strand at 3351.190 kb on - strand at 3351.190 kb on - strand at 3351.267 kb on + strand at 3351.267 kb on + strand at 3351.477 kb on + strand, within IAI46_15980 at 3351.478 kb on - strand, within IAI46_15980 at 3351.478 kb on - strand, within IAI46_15980 at 3351.478 kb on - strand, within IAI46_15980 at 3351.543 kb on + strand, within IAI46_15980 at 3351.588 kb on - strand, within IAI46_15980 at 3351.625 kb on - strand, within IAI46_15980 at 3351.690 kb on - strand, within IAI46_15980 at 3351.693 kb on + strand, within IAI46_15980 at 3351.712 kb on - strand, within IAI46_15980 at 3351.844 kb on - strand, within IAI46_15980 at 3351.887 kb on - strand, within IAI46_15980 at 3352.162 kb on - strand, within IAI46_15980 at 3352.162 kb on - strand, within IAI46_15980 at 3352.183 kb on - strand, within IAI46_15980 at 3352.183 kb on - strand, within IAI46_15980 at 3352.203 kb on - strand, within IAI46_15980 at 3352.203 kb on - strand, within IAI46_15980 at 3352.204 kb on + strand, within IAI46_15980 at 3352.205 kb on - strand, within IAI46_15980 at 3352.205 kb on - strand, within IAI46_15980 at 3352.211 kb on - strand, within IAI46_15980 at 3352.232 kb on - strand, within IAI46_15980 at 3352.265 kb on + strand, within IAI46_15980 at 3352.266 kb on - strand, within IAI46_15980 at 3352.266 kb on - strand, within IAI46_15980 at 3352.331 kb on + strand, within IAI46_15980 at 3352.332 kb on - strand, within IAI46_15980 at 3352.429 kb on - strand at 3352.463 kb on + strand at 3352.463 kb on + strand at 3352.513 kb on + strand at 3352.513 kb on + strand at 3352.513 kb on + strand at 3352.573 kb on - strand, within IAI46_15985 at 3352.583 kb on - strand, within IAI46_15985 at 3352.600 kb on + strand, within IAI46_15985 at 3352.601 kb on - strand, within IAI46_15985 at 3352.700 kb on + strand, within IAI46_15985 at 3352.758 kb on + strand, within IAI46_15985 at 3352.759 kb on - strand, within IAI46_15985 at 3352.790 kb on - strand, within IAI46_15985 at 3352.790 kb on - strand, within IAI46_15985 at 3352.815 kb on + strand, within IAI46_15985 at 3352.816 kb on - strand, within IAI46_15985 at 3352.874 kb on + strand at 3352.875 kb on - strand at 3352.913 kb on - strand at 3352.980 kb on - strand at 3353.031 kb on - strand at 3353.130 kb on + strand at 3353.196 kb on + strand, within IAI46_15990 at 3353.348 kb on + strand, within IAI46_15990 at 3353.384 kb on - strand, within IAI46_15990 at 3353.427 kb on + strand, within IAI46_15990
Per-strain Table
Position Strand Gene LocusTag Fraction Cadmium acetate 40 uM, aerobic remove 3,350,318 - +0.1 3,350,367 + +0.9 3,350,544 + IAI46_15970 0.56 -1.6 3,350,699 - -2.7 3,350,898 + IAI46_15975 0.37 +0.5 3,350,898 + IAI46_15975 0.37 -3.9 3,350,899 - IAI46_15975 0.37 -1.9 3,350,899 - IAI46_15975 0.37 -0.2 3,350,992 + IAI46_15975 0.59 -0.7 3,351,032 + IAI46_15975 0.68 +1.1 3,351,032 + IAI46_15975 0.68 +0.1 3,351,112 - IAI46_15975 0.86 +0.7 3,351,189 + +1.3 3,351,189 + -1.2 3,351,190 - +0.6 3,351,190 - +0.6 3,351,190 - -0.3 3,351,190 - +0.8 3,351,267 + -1.9 3,351,267 + +0.6 3,351,477 + IAI46_15980 0.19 +2.5 3,351,478 - IAI46_15980 0.19 +1.8 3,351,478 - IAI46_15980 0.19 -0.2 3,351,478 - IAI46_15980 0.19 -1.2 3,351,543 + IAI46_15980 0.25 +1.7 3,351,588 - IAI46_15980 0.28 +1.0 3,351,625 - IAI46_15980 0.31 +1.1 3,351,690 - IAI46_15980 0.36 +1.4 3,351,693 + IAI46_15980 0.37 -1.1 3,351,712 - IAI46_15980 0.38 -0.0 3,351,844 - IAI46_15980 0.48 -1.7 3,351,887 - IAI46_15980 0.52 +1.7 3,352,162 - IAI46_15980 0.74 +1.0 3,352,162 - IAI46_15980 0.74 -0.9 3,352,183 - IAI46_15980 0.75 -0.3 3,352,183 - IAI46_15980 0.75 +0.5 3,352,203 - IAI46_15980 0.77 +0.5 3,352,203 - IAI46_15980 0.77 -1.9 3,352,204 + IAI46_15980 0.77 -1.2 3,352,205 - IAI46_15980 0.77 +1.2 3,352,205 - IAI46_15980 0.77 -0.5 3,352,211 - IAI46_15980 0.78 +0.3 3,352,232 - IAI46_15980 0.79 +1.9 3,352,265 + IAI46_15980 0.82 +0.6 3,352,266 - IAI46_15980 0.82 +1.4 3,352,266 - IAI46_15980 0.82 +1.1 3,352,331 + IAI46_15980 0.87 +0.4 3,352,332 - IAI46_15980 0.87 +0.0 3,352,429 - -1.6 3,352,463 + -1.2 3,352,463 + -0.7 3,352,513 + +0.1 3,352,513 + -1.5 3,352,513 + +1.7 3,352,573 - IAI46_15985 0.22 -2.7 3,352,583 - IAI46_15985 0.24 -1.9 3,352,600 + IAI46_15985 0.28 -0.0 3,352,601 - IAI46_15985 0.28 +2.6 3,352,700 + IAI46_15985 0.51 -1.6 3,352,758 + IAI46_15985 0.64 +1.2 3,352,759 - IAI46_15985 0.65 +1.5 3,352,790 - IAI46_15985 0.72 +0.1 3,352,790 - IAI46_15985 0.72 -1.7 3,352,815 + IAI46_15985 0.77 -1.1 3,352,816 - IAI46_15985 0.78 +1.5 3,352,874 + -0.2 3,352,875 - -1.7 3,352,913 - -0.5 3,352,980 - -2.1 3,353,031 - +0.1 3,353,130 + +0.3 3,353,196 + IAI46_15990 0.18 -0.1 3,353,348 + IAI46_15990 0.56 -0.5 3,353,384 - IAI46_15990 0.64 -0.9 3,353,427 + IAI46_15990 0.75 -2.6
Or see this region's nucleotide sequence