Experiment: KB with Trimethoprim 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2467 and Psyr_2468 overlap by 4 nucleotides Psyr_2468 and Psyr_2469 are separated by 14 nucleotides Psyr_2469 and Psyr_2470 are separated by 13 nucleotides
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636
_2468
Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258
_2469
Psyr_2470: Psyr_2470 - isovaleryl-CoA dehydrogenase, at 2,863,272 to 2,864,435
_2470
Position (kb)
2861
2862
2863
2864 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2860.675 kb on - strand at 2861.140 kb on + strand, within Psyr_2468 at 2861.348 kb on - strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.677 kb on + strand at 2861.677 kb on + strand at 2861.784 kb on + strand at 2861.784 kb on + strand at 2861.784 kb on + strand at 2861.806 kb on - strand at 2861.957 kb on + strand, within Psyr_2469 at 2861.957 kb on + strand, within Psyr_2469 at 2861.958 kb on - strand, within Psyr_2469 at 2861.973 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.106 kb on - strand, within Psyr_2469 at 2862.391 kb on - strand, within Psyr_2469 at 2862.837 kb on + strand, within Psyr_2469 at 2862.838 kb on - strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.393 kb on + strand, within Psyr_2470 at 2863.393 kb on + strand, within Psyr_2470 at 2863.456 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.578 kb on + strand, within Psyr_2470 at 2863.578 kb on + strand, within Psyr_2470 at 2863.630 kb on + strand, within Psyr_2470 at 2863.631 kb on - strand, within Psyr_2470 at 2863.686 kb on + strand, within Psyr_2470 at 2863.915 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2864.092 kb on + strand, within Psyr_2470
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 15.6 ug/ml remove 2,860,675 - +0.7 2,861,140 + Psyr_2468 0.43 -1.3 2,861,348 - Psyr_2468 0.67 -1.7 2,861,392 + Psyr_2468 0.72 -0.8 2,861,392 + Psyr_2468 0.72 +2.7 2,861,392 + Psyr_2468 0.72 -0.4 2,861,393 - Psyr_2468 0.72 -1.1 2,861,393 - Psyr_2468 0.72 -0.7 2,861,496 + Psyr_2468 0.84 -1.3 2,861,496 + Psyr_2468 0.84 +0.3 2,861,496 + Psyr_2468 0.84 -0.7 2,861,610 + -0.7 2,861,610 + +0.6 2,861,610 + -0.4 2,861,677 + -1.7 2,861,677 + -0.3 2,861,784 + -0.1 2,861,784 + +0.4 2,861,784 + +0.5 2,861,806 - -1.4 2,861,957 + Psyr_2469 0.19 +0.7 2,861,957 + Psyr_2469 0.19 -1.2 2,861,958 - Psyr_2469 0.19 +1.8 2,861,973 + Psyr_2469 0.20 -0.9 2,862,105 + Psyr_2469 0.28 -1.5 2,862,105 + Psyr_2469 0.28 +1.7 2,862,105 + Psyr_2469 0.28 -0.3 2,862,106 - Psyr_2469 0.28 -0.8 2,862,391 - Psyr_2469 0.46 -0.1 2,862,837 + Psyr_2469 0.74 -0.3 2,862,838 - Psyr_2469 0.74 -0.6 2,863,016 + Psyr_2469 0.85 -0.6 2,863,016 + Psyr_2469 0.85 +1.1 2,863,016 + Psyr_2469 0.85 +0.1 2,863,016 + Psyr_2469 0.85 +0.2 2,863,016 + Psyr_2469 0.85 -0.7 2,863,017 - Psyr_2469 0.85 +0.7 2,863,017 - Psyr_2469 0.85 -1.7 2,863,017 - Psyr_2469 0.85 +1.1 2,863,017 - Psyr_2469 0.85 -0.5 2,863,393 + Psyr_2470 0.10 -0.5 2,863,393 + Psyr_2470 0.10 -0.7 2,863,456 + Psyr_2470 0.16 -1.3 2,863,555 + Psyr_2470 0.24 -0.7 2,863,555 + Psyr_2470 0.24 -0.2 2,863,555 + Psyr_2470 0.24 +0.9 2,863,555 + Psyr_2470 0.24 -0.9 2,863,556 - Psyr_2470 0.24 +0.6 2,863,556 - Psyr_2470 0.24 +1.0 2,863,556 - Psyr_2470 0.24 -0.3 2,863,556 - Psyr_2470 0.24 -1.8 2,863,556 - Psyr_2470 0.24 -0.5 2,863,556 - Psyr_2470 0.24 -0.9 2,863,556 - Psyr_2470 0.24 +0.9 2,863,578 + Psyr_2470 0.26 -0.7 2,863,578 + Psyr_2470 0.26 +0.7 2,863,630 + Psyr_2470 0.31 +2.7 2,863,631 - Psyr_2470 0.31 -0.3 2,863,686 + Psyr_2470 0.36 -2.5 2,863,915 + Psyr_2470 0.55 -0.7 2,863,924 + Psyr_2470 0.56 -1.2 2,863,924 + Psyr_2470 0.56 +0.1 2,863,924 + Psyr_2470 0.56 -0.3 2,863,924 + Psyr_2470 0.56 +1.8 2,863,924 + Psyr_2470 0.56 -0.1 2,863,924 + Psyr_2470 0.56 -0.9 2,863,925 - Psyr_2470 0.56 -0.1 2,863,925 - Psyr_2470 0.56 -0.7 2,863,925 - Psyr_2470 0.56 -0.1 2,863,925 - Psyr_2470 0.56 +0.7 2,864,092 + Psyr_2470 0.70 +1.8
Or see this region's nucleotide sequence