Experiment: KB with Trimethoprim 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2465 and Psyr_2466 are separated by 70 nucleotides Psyr_2466 and Psyr_2467 are separated by 96 nucleotides Psyr_2467 and Psyr_2468 overlap by 4 nucleotides Psyr_2468 and Psyr_2469 are separated by 14 nucleotides
Psyr_2465: Psyr_2465 - Acyltransferase 3, at 2,857,032 to 2,858,054
_2465
Psyr_2466: Psyr_2466 - HesB/YadR/YfhF:Nitrogen-fixing NifU, C-terminal, at 2,858,125 to 2,858,718
_2466
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636
_2468
Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258
_2469
Position (kb)
2858
2859
2860
2861 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2857.836 kb on + strand, within Psyr_2465 at 2857.837 kb on - strand, within Psyr_2465 at 2857.845 kb on + strand, within Psyr_2465 at 2857.846 kb on - strand, within Psyr_2465 at 2857.869 kb on + strand, within Psyr_2465 at 2858.062 kb on - strand at 2858.710 kb on + strand at 2858.711 kb on - strand at 2858.711 kb on - strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.714 kb on - strand at 2858.716 kb on + strand at 2858.717 kb on - strand at 2859.105 kb on - strand, within Psyr_2467 at 2859.105 kb on - strand, within Psyr_2467 at 2859.112 kb on - strand, within Psyr_2467 at 2859.191 kb on - strand, within Psyr_2467 at 2859.605 kb on + strand, within Psyr_2467 at 2859.680 kb on + strand, within Psyr_2467 at 2859.849 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.352 kb on + strand, within Psyr_2467 at 2860.575 kb on - strand at 2860.675 kb on - strand at 2861.140 kb on + strand, within Psyr_2468 at 2861.348 kb on - strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.677 kb on + strand at 2861.677 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 15.6 ug/ml remove 2,857,836 + Psyr_2465 0.79 +0.3 2,857,837 - Psyr_2465 0.79 +0.1 2,857,845 + Psyr_2465 0.79 -0.9 2,857,846 - Psyr_2465 0.80 -1.1 2,857,869 + Psyr_2465 0.82 -0.1 2,858,062 - -0.6 2,858,710 + +0.1 2,858,711 - +0.2 2,858,711 - -0.7 2,858,713 + -0.5 2,858,713 + +0.7 2,858,713 + +0.4 2,858,714 - +1.5 2,858,716 + +0.2 2,858,717 - -1.9 2,859,105 - Psyr_2467 0.15 -1.3 2,859,105 - Psyr_2467 0.15 -0.1 2,859,112 - Psyr_2467 0.15 +0.7 2,859,191 - Psyr_2467 0.19 +1.2 2,859,605 + Psyr_2467 0.41 -0.1 2,859,680 + Psyr_2467 0.44 +1.7 2,859,849 - Psyr_2467 0.53 -0.4 2,860,149 - Psyr_2467 0.68 +0.7 2,860,149 - Psyr_2467 0.68 +2.7 2,860,149 - Psyr_2467 0.68 +0.9 2,860,352 + Psyr_2467 0.79 -0.5 2,860,575 - -0.1 2,860,675 - +0.7 2,861,140 + Psyr_2468 0.43 -1.3 2,861,348 - Psyr_2468 0.67 -1.7 2,861,392 + Psyr_2468 0.72 -0.8 2,861,392 + Psyr_2468 0.72 +2.7 2,861,392 + Psyr_2468 0.72 -0.4 2,861,393 - Psyr_2468 0.72 -1.1 2,861,393 - Psyr_2468 0.72 -0.7 2,861,496 + Psyr_2468 0.84 -1.3 2,861,496 + Psyr_2468 0.84 +0.3 2,861,496 + Psyr_2468 0.84 -0.7 2,861,610 + -0.7 2,861,610 + +0.6 2,861,610 + -0.4 2,861,677 + -1.7 2,861,677 + -0.3
Or see this region's nucleotide sequence