Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_2467

Experiment: KB with Trimethoprim 15.6 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2465 and Psyr_2466 are separated by 70 nucleotidesPsyr_2466 and Psyr_2467 are separated by 96 nucleotidesPsyr_2467 and Psyr_2468 overlap by 4 nucleotidesPsyr_2468 and Psyr_2469 are separated by 14 nucleotides Psyr_2465: Psyr_2465 - Acyltransferase 3, at 2,857,032 to 2,858,054 _2465 Psyr_2466: Psyr_2466 - HesB/YadR/YfhF:Nitrogen-fixing NifU, C-terminal, at 2,858,125 to 2,858,718 _2466 Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764 _2467 Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636 _2468 Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258 _2469 Position (kb) 2858 2859 2860 2861Strain fitness (log2 ratio) -2 -1 0 1 2at 2857.836 kb on + strand, within Psyr_2465at 2857.837 kb on - strand, within Psyr_2465at 2857.845 kb on + strand, within Psyr_2465at 2857.846 kb on - strand, within Psyr_2465at 2857.869 kb on + strand, within Psyr_2465at 2858.062 kb on - strandat 2858.710 kb on + strandat 2858.711 kb on - strandat 2858.711 kb on - strandat 2858.713 kb on + strandat 2858.713 kb on + strandat 2858.713 kb on + strandat 2858.714 kb on - strandat 2858.716 kb on + strandat 2858.717 kb on - strandat 2859.105 kb on - strand, within Psyr_2467at 2859.105 kb on - strand, within Psyr_2467at 2859.112 kb on - strand, within Psyr_2467at 2859.191 kb on - strand, within Psyr_2467at 2859.605 kb on + strand, within Psyr_2467at 2859.680 kb on + strand, within Psyr_2467at 2859.849 kb on - strand, within Psyr_2467at 2860.149 kb on - strand, within Psyr_2467at 2860.149 kb on - strand, within Psyr_2467at 2860.149 kb on - strand, within Psyr_2467at 2860.352 kb on + strand, within Psyr_2467at 2860.575 kb on - strandat 2860.675 kb on - strandat 2861.140 kb on + strand, within Psyr_2468at 2861.348 kb on - strand, within Psyr_2468at 2861.392 kb on + strand, within Psyr_2468at 2861.392 kb on + strand, within Psyr_2468at 2861.392 kb on + strand, within Psyr_2468at 2861.393 kb on - strand, within Psyr_2468at 2861.393 kb on - strand, within Psyr_2468at 2861.496 kb on + strand, within Psyr_2468at 2861.496 kb on + strand, within Psyr_2468at 2861.496 kb on + strand, within Psyr_2468at 2861.610 kb on + strandat 2861.610 kb on + strandat 2861.610 kb on + strandat 2861.677 kb on + strandat 2861.677 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Trimethoprim 15.6 ug/ml
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2,857,836 + Psyr_2465 0.79 +0.3
2,857,837 - Psyr_2465 0.79 +0.1
2,857,845 + Psyr_2465 0.79 -0.9
2,857,846 - Psyr_2465 0.80 -1.1
2,857,869 + Psyr_2465 0.82 -0.1
2,858,062 - -0.6
2,858,710 + +0.1
2,858,711 - +0.2
2,858,711 - -0.7
2,858,713 + -0.5
2,858,713 + +0.7
2,858,713 + +0.4
2,858,714 - +1.5
2,858,716 + +0.2
2,858,717 - -1.9
2,859,105 - Psyr_2467 0.15 -1.3
2,859,105 - Psyr_2467 0.15 -0.1
2,859,112 - Psyr_2467 0.15 +0.7
2,859,191 - Psyr_2467 0.19 +1.2
2,859,605 + Psyr_2467 0.41 -0.1
2,859,680 + Psyr_2467 0.44 +1.7
2,859,849 - Psyr_2467 0.53 -0.4
2,860,149 - Psyr_2467 0.68 +0.7
2,860,149 - Psyr_2467 0.68 +2.7
2,860,149 - Psyr_2467 0.68 +0.9
2,860,352 + Psyr_2467 0.79 -0.5
2,860,575 - -0.1
2,860,675 - +0.7
2,861,140 + Psyr_2468 0.43 -1.3
2,861,348 - Psyr_2468 0.67 -1.7
2,861,392 + Psyr_2468 0.72 -0.8
2,861,392 + Psyr_2468 0.72 +2.7
2,861,392 + Psyr_2468 0.72 -0.4
2,861,393 - Psyr_2468 0.72 -1.1
2,861,393 - Psyr_2468 0.72 -0.7
2,861,496 + Psyr_2468 0.84 -1.3
2,861,496 + Psyr_2468 0.84 +0.3
2,861,496 + Psyr_2468 0.84 -0.7
2,861,610 + -0.7
2,861,610 + +0.6
2,861,610 + -0.4
2,861,677 + -1.7
2,861,677 + -0.3

Or see this region's nucleotide sequence