Experiment: KB with Trimethoprim 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2428 and Psyr_RNA42 are separated by 578 nucleotides Psyr_RNA42 and Psyr_2429 are separated by 134 nucleotides Psyr_2429 and Psyr_2430 are separated by 89 nucleotides Psyr_2430 and Psyr_2431 are separated by 302 nucleotides
Psyr_2428: Psyr_2428 - GCN5-related N-acetyltransferase, at 2,809,850 to 2,810,326
_2428
Psyr_RNA42: Psyr_RNA42 - tRNA-Val, at 2,810,905 to 2,810,981
_RNA42
Psyr_2429: Psyr_2429 - conserved hypothetical protein, at 2,811,116 to 2,812,177
_2429
Psyr_2430: Psyr_2430 - NUDIX hydrolase, at 2,812,267 to 2,812,665
_2430
Psyr_2431: Psyr_2431 - Zinc-containing alcohol dehydrogenase superfamily, at 2,812,968 to 2,814,092
_2431
Position (kb)
2811
2812
2813 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2810.132 kb on - strand, within Psyr_2428 at 2810.151 kb on - strand, within Psyr_2428 at 2810.153 kb on + strand, within Psyr_2428 at 2810.154 kb on - strand, within Psyr_2428 at 2810.320 kb on + strand at 2810.373 kb on + strand at 2810.373 kb on + strand at 2810.374 kb on - strand at 2810.416 kb on + strand at 2810.485 kb on - strand at 2810.491 kb on - strand at 2810.647 kb on + strand at 2810.654 kb on + strand at 2810.654 kb on + strand at 2810.655 kb on - strand at 2810.655 kb on - strand at 2810.713 kb on - strand at 2810.722 kb on - strand at 2810.722 kb on - strand at 2810.725 kb on + strand at 2810.726 kb on - strand at 2810.837 kb on - strand at 2810.837 kb on - strand at 2811.060 kb on + strand at 2811.061 kb on - strand at 2811.601 kb on + strand, within Psyr_2429 at 2811.616 kb on - strand, within Psyr_2429 at 2811.698 kb on - strand, within Psyr_2429 at 2811.698 kb on - strand, within Psyr_2429 at 2811.745 kb on - strand, within Psyr_2429 at 2811.747 kb on + strand, within Psyr_2429 at 2811.747 kb on + strand, within Psyr_2429 at 2811.748 kb on - strand, within Psyr_2429 at 2811.748 kb on - strand, within Psyr_2429 at 2811.837 kb on + strand, within Psyr_2429 at 2811.837 kb on + strand, within Psyr_2429 at 2811.838 kb on - strand, within Psyr_2429 at 2812.178 kb on - strand at 2812.205 kb on + strand at 2812.205 kb on + strand at 2812.205 kb on + strand at 2812.205 kb on + strand at 2812.206 kb on - strand at 2812.206 kb on - strand at 2812.256 kb on + strand at 2812.268 kb on + strand at 2812.319 kb on + strand, within Psyr_2430 at 2812.319 kb on + strand, within Psyr_2430 at 2812.320 kb on - strand, within Psyr_2430 at 2812.479 kb on - strand, within Psyr_2430 at 2812.570 kb on + strand, within Psyr_2430 at 2812.571 kb on - strand, within Psyr_2430 at 2812.571 kb on - strand, within Psyr_2430 at 2812.571 kb on - strand, within Psyr_2430 at 2812.571 kb on - strand, within Psyr_2430 at 2812.616 kb on + strand, within Psyr_2430 at 2812.617 kb on - strand, within Psyr_2430 at 2812.617 kb on - strand, within Psyr_2430 at 2812.666 kb on - strand at 2812.682 kb on - strand at 2812.701 kb on + strand at 2812.701 kb on + strand at 2812.701 kb on + strand at 2812.702 kb on - strand at 2812.702 kb on - strand at 2812.738 kb on - strand at 2812.762 kb on - strand at 2812.769 kb on - strand at 2812.870 kb on + strand at 2812.870 kb on + strand at 2812.871 kb on - strand at 2812.875 kb on + strand at 2812.924 kb on + strand at 2813.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 15.6 ug/ml remove 2,810,132 - Psyr_2428 0.59 +0.9 2,810,151 - Psyr_2428 0.63 +0.4 2,810,153 + Psyr_2428 0.64 -0.6 2,810,154 - Psyr_2428 0.64 -0.2 2,810,320 + -0.6 2,810,373 + +0.1 2,810,373 + +0.5 2,810,374 - -1.5 2,810,416 + -0.2 2,810,485 - +0.5 2,810,491 - +0.7 2,810,647 + -0.3 2,810,654 + +0.3 2,810,654 + +1.4 2,810,655 - -0.5 2,810,655 - +1.0 2,810,713 - +0.1 2,810,722 - -0.3 2,810,722 - +0.7 2,810,725 + +0.4 2,810,726 - +1.1 2,810,837 - -0.8 2,810,837 - -0.3 2,811,060 + -0.7 2,811,061 - -1.7 2,811,601 + Psyr_2429 0.46 -0.4 2,811,616 - Psyr_2429 0.47 +1.3 2,811,698 - Psyr_2429 0.55 +0.8 2,811,698 - Psyr_2429 0.55 +2.2 2,811,745 - Psyr_2429 0.59 +1.8 2,811,747 + Psyr_2429 0.59 +0.9 2,811,747 + Psyr_2429 0.59 -0.8 2,811,748 - Psyr_2429 0.60 -0.1 2,811,748 - Psyr_2429 0.60 -0.5 2,811,837 + Psyr_2429 0.68 +1.5 2,811,837 + Psyr_2429 0.68 +0.2 2,811,838 - Psyr_2429 0.68 -0.7 2,812,178 - +1.0 2,812,205 + -0.6 2,812,205 + -0.3 2,812,205 + +0.5 2,812,205 + +0.3 2,812,206 - -0.4 2,812,206 - +0.1 2,812,256 + +1.0 2,812,268 + +0.7 2,812,319 + Psyr_2430 0.13 -0.5 2,812,319 + Psyr_2430 0.13 -0.5 2,812,320 - Psyr_2430 0.13 -0.3 2,812,479 - Psyr_2430 0.53 -1.7 2,812,570 + Psyr_2430 0.76 +0.8 2,812,571 - Psyr_2430 0.76 -0.4 2,812,571 - Psyr_2430 0.76 -1.3 2,812,571 - Psyr_2430 0.76 -1.6 2,812,571 - Psyr_2430 0.76 -0.6 2,812,616 + Psyr_2430 0.87 +0.2 2,812,617 - Psyr_2430 0.88 +0.4 2,812,617 - Psyr_2430 0.88 -3.0 2,812,666 - -0.1 2,812,682 - -1.9 2,812,701 + +0.3 2,812,701 + +0.1 2,812,701 + +0.5 2,812,702 - +0.7 2,812,702 - +0.7 2,812,738 - +0.0 2,812,762 - -0.2 2,812,769 - -1.7 2,812,870 + +0.7 2,812,870 + +0.3 2,812,871 - +0.2 2,812,875 + +1.0 2,812,924 + +0.2 2,813,033 - -0.1
Or see this region's nucleotide sequence