Experiment: KB with Trimethoprim 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1027 and Psyr_1028 are separated by 81 nucleotides Psyr_1028 and Psyr_1029 are separated by 31 nucleotides Psyr_1029 and Psyr_1030 overlap by 4 nucleotides
Psyr_1027: Psyr_1027 - conserved hypothetical protein, at 1,157,518 to 1,158,321
_1027
Psyr_1028: Psyr_1028 - conserved hypothetical protein, at 1,158,403 to 1,159,275
_1028
Psyr_1029: Psyr_1029 - Peptidase aspartic, active site, at 1,159,307 to 1,161,271
_1029
Psyr_1030: Psyr_1030 - bacteriophage N4 adsorption protein B, at 1,161,268 to 1,163,388
_1030
Position (kb) 1159
1160
1161
1162 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1158.366 kb on - strand at 1158.366 kb on - strand at 1158.404 kb on + strand at 1158.404 kb on + strand at 1158.404 kb on + strand at 1158.405 kb on - strand at 1158.405 kb on - strand at 1158.405 kb on - strand at 1158.422 kb on - strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.441 kb on - strand at 1158.441 kb on - strand at 1158.441 kb on - strand at 1158.441 kb on - strand at 1158.461 kb on + strand at 1158.462 kb on - strand at 1158.462 kb on - strand at 1158.608 kb on + strand, within Psyr_1028 at 1158.608 kb on + strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.638 kb on + strand, within Psyr_1028 at 1158.831 kb on - strand, within Psyr_1028 at 1158.831 kb on - strand, within Psyr_1028 at 1159.102 kb on + strand, within Psyr_1028 at 1159.102 kb on + strand, within Psyr_1028 at 1159.103 kb on - strand, within Psyr_1028 at 1159.109 kb on - strand, within Psyr_1028 at 1159.175 kb on + strand, within Psyr_1028 at 1159.175 kb on + strand, within Psyr_1028 at 1159.280 kb on + strand at 1159.280 kb on + strand at 1159.281 kb on - strand at 1159.281 kb on - strand at 1159.395 kb on + strand at 1159.395 kb on + strand at 1159.395 kb on + strand at 1159.395 kb on + strand at 1159.395 kb on + strand at 1159.395 kb on + strand at 1159.396 kb on - strand at 1159.396 kb on - strand at 1159.396 kb on - strand at 1159.396 kb on - strand at 1159.396 kb on - strand at 1159.396 kb on - strand at 1159.413 kb on + strand at 1159.413 kb on + strand at 1159.413 kb on + strand at 1159.413 kb on + strand at 1159.413 kb on + strand at 1159.413 kb on + strand at 1159.413 kb on + strand at 1159.413 kb on + strand at 1159.414 kb on - strand at 1159.414 kb on - strand at 1159.414 kb on - strand at 1159.414 kb on - strand at 1159.414 kb on - strand at 1159.414 kb on - strand at 1159.414 kb on - strand at 1159.417 kb on + strand at 1159.417 kb on + strand at 1159.418 kb on - strand at 1159.418 kb on - strand at 1159.418 kb on - strand at 1159.471 kb on + strand at 1159.647 kb on + strand, within Psyr_1029 at 1159.665 kb on + strand, within Psyr_1029 at 1159.665 kb on + strand, within Psyr_1029 at 1159.665 kb on + strand, within Psyr_1029 at 1159.665 kb on + strand, within Psyr_1029 at 1159.665 kb on + strand, within Psyr_1029 at 1159.666 kb on - strand, within Psyr_1029 at 1159.692 kb on - strand, within Psyr_1029 at 1160.041 kb on - strand, within Psyr_1029 at 1160.146 kb on - strand, within Psyr_1029 at 1160.167 kb on - strand, within Psyr_1029 at 1160.270 kb on - strand, within Psyr_1029 at 1160.310 kb on + strand, within Psyr_1029 at 1160.310 kb on + strand, within Psyr_1029 at 1160.310 kb on + strand, within Psyr_1029 at 1160.310 kb on + strand, within Psyr_1029 at 1160.310 kb on + strand, within Psyr_1029 at 1160.310 kb on + strand, within Psyr_1029 at 1160.311 kb on - strand, within Psyr_1029 at 1160.311 kb on - strand, within Psyr_1029 at 1160.311 kb on - strand, within Psyr_1029 at 1160.315 kb on + strand, within Psyr_1029 at 1160.315 kb on + strand, within Psyr_1029 at 1160.407 kb on - strand, within Psyr_1029 at 1160.407 kb on - strand, within Psyr_1029 at 1160.407 kb on - strand, within Psyr_1029 at 1160.615 kb on + strand, within Psyr_1029 at 1160.710 kb on - strand, within Psyr_1029 at 1160.947 kb on - strand, within Psyr_1029 at 1160.947 kb on - strand, within Psyr_1029 at 1161.000 kb on + strand, within Psyr_1029 at 1161.027 kb on + strand, within Psyr_1029 at 1161.028 kb on - strand, within Psyr_1029 at 1161.049 kb on + strand, within Psyr_1029 at 1161.050 kb on - strand, within Psyr_1029 at 1161.138 kb on + strand at 1161.138 kb on + strand at 1161.147 kb on + strand at 1161.147 kb on + strand at 1161.147 kb on + strand at 1161.147 kb on + strand at 1161.147 kb on + strand at 1161.147 kb on + strand at 1161.147 kb on + strand at 1161.147 kb on + strand at 1161.148 kb on - strand at 1161.148 kb on - strand at 1161.148 kb on - strand at 1161.148 kb on - strand at 1161.148 kb on - strand at 1161.168 kb on + strand at 1161.169 kb on - strand at 1161.272 kb on - strand at 1161.272 kb on - strand at 1161.272 kb on - strand at 1161.371 kb on + strand at 1161.372 kb on - strand at 1162.097 kb on + strand, within Psyr_1030 at 1162.115 kb on + strand, within Psyr_1030 at 1162.115 kb on + strand, within Psyr_1030 at 1162.116 kb on - strand, within Psyr_1030 at 1162.116 kb on - strand, within Psyr_1030 at 1162.116 kb on - strand, within Psyr_1030 at 1162.169 kb on + strand, within Psyr_1030 at 1162.170 kb on - strand, within Psyr_1030 at 1162.170 kb on - strand, within Psyr_1030 at 1162.170 kb on - strand, within Psyr_1030 at 1162.213 kb on - strand, within Psyr_1030 at 1162.213 kb on - strand, within Psyr_1030
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 15.6 ug/ml remove 1,158,366 - +0.3 1,158,366 - -1.0 1,158,404 + -1.2 1,158,404 + -0.3 1,158,404 + +0.6 1,158,405 - +0.5 1,158,405 - +0.0 1,158,405 - -0.1 1,158,422 - +1.0 1,158,440 + -0.7 1,158,440 + -0.7 1,158,440 + -0.2 1,158,440 + -0.1 1,158,440 + +0.8 1,158,440 + -0.1 1,158,440 + -0.1 1,158,440 + +0.6 1,158,441 - +0.3 1,158,441 - +1.5 1,158,441 - +0.5 1,158,441 - -0.5 1,158,461 + -0.6 1,158,462 - -0.7 1,158,462 - -0.4 1,158,608 + Psyr_1028 0.23 +0.9 1,158,608 + Psyr_1028 0.23 -0.3 1,158,609 - Psyr_1028 0.24 +0.3 1,158,609 - Psyr_1028 0.24 -0.2 1,158,609 - Psyr_1028 0.24 -1.2 1,158,609 - Psyr_1028 0.24 +1.2 1,158,609 - Psyr_1028 0.24 +0.1 1,158,638 + Psyr_1028 0.27 +0.8 1,158,831 - Psyr_1028 0.49 +0.7 1,158,831 - Psyr_1028 0.49 -0.4 1,159,102 + Psyr_1028 0.80 -1.1 1,159,102 + Psyr_1028 0.80 -0.6 1,159,103 - Psyr_1028 0.80 -0.1 1,159,109 - Psyr_1028 0.81 -2.0 1,159,175 + Psyr_1028 0.88 -0.4 1,159,175 + Psyr_1028 0.88 -0.1 1,159,280 + +1.5 1,159,280 + +0.9 1,159,281 - +0.5 1,159,281 - -0.5 1,159,395 + +0.2 1,159,395 + +0.9 1,159,395 + +0.9 1,159,395 + +0.4 1,159,395 + +1.6 1,159,395 + +0.1 1,159,396 - +2.2 1,159,396 - +0.5 1,159,396 - +0.3 1,159,396 - +0.6 1,159,396 - +0.9 1,159,396 - +1.6 1,159,413 + +1.1 1,159,413 + +0.1 1,159,413 + +0.4 1,159,413 + -0.1 1,159,413 + -0.3 1,159,413 + -0.1 1,159,413 + +0.1 1,159,413 + -1.3 1,159,414 - -0.5 1,159,414 - -0.3 1,159,414 - +1.9 1,159,414 - -0.3 1,159,414 - -0.7 1,159,414 - -0.4 1,159,414 - +1.5 1,159,417 + -0.5 1,159,417 + -0.2 1,159,418 - +0.2 1,159,418 - +0.9 1,159,418 - +0.5 1,159,471 + +0.4 1,159,647 + Psyr_1029 0.17 +0.9 1,159,665 + Psyr_1029 0.18 +0.5 1,159,665 + Psyr_1029 0.18 -3.0 1,159,665 + Psyr_1029 0.18 -0.1 1,159,665 + Psyr_1029 0.18 +0.3 1,159,665 + Psyr_1029 0.18 +0.3 1,159,666 - Psyr_1029 0.18 +0.3 1,159,692 - Psyr_1029 0.20 +0.1 1,160,041 - Psyr_1029 0.37 -0.4 1,160,146 - Psyr_1029 0.43 +0.4 1,160,167 - Psyr_1029 0.44 +0.7 1,160,270 - Psyr_1029 0.49 -1.7 1,160,310 + Psyr_1029 0.51 -0.9 1,160,310 + Psyr_1029 0.51 +0.8 1,160,310 + Psyr_1029 0.51 -1.1 1,160,310 + Psyr_1029 0.51 +0.8 1,160,310 + Psyr_1029 0.51 -0.1 1,160,310 + Psyr_1029 0.51 -0.4 1,160,311 - Psyr_1029 0.51 -0.1 1,160,311 - Psyr_1029 0.51 +1.5 1,160,311 - Psyr_1029 0.51 +0.0 1,160,315 + Psyr_1029 0.51 +0.1 1,160,315 + Psyr_1029 0.51 -0.6 1,160,407 - Psyr_1029 0.56 -1.4 1,160,407 - Psyr_1029 0.56 +0.5 1,160,407 - Psyr_1029 0.56 +0.9 1,160,615 + Psyr_1029 0.67 +0.5 1,160,710 - Psyr_1029 0.71 +1.9 1,160,947 - Psyr_1029 0.83 +0.0 1,160,947 - Psyr_1029 0.83 -0.9 1,161,000 + Psyr_1029 0.86 +1.1 1,161,027 + Psyr_1029 0.88 -0.7 1,161,028 - Psyr_1029 0.88 -0.5 1,161,049 + Psyr_1029 0.89 +0.6 1,161,050 - Psyr_1029 0.89 +0.1 1,161,138 + +1.2 1,161,138 + -1.1 1,161,147 + +0.1 1,161,147 + +0.5 1,161,147 + -0.3 1,161,147 + -1.4 1,161,147 + -0.1 1,161,147 + -0.4 1,161,147 + -0.2 1,161,147 + -0.4 1,161,148 - -0.1 1,161,148 - -0.3 1,161,148 - -0.0 1,161,148 - +0.9 1,161,148 - +0.4 1,161,168 + +0.3 1,161,169 - -1.0 1,161,272 - +0.1 1,161,272 - -0.3 1,161,272 - +1.6 1,161,371 + -0.4 1,161,372 - +0.6 1,162,097 + Psyr_1030 0.39 +0.1 1,162,115 + Psyr_1030 0.40 -0.3 1,162,115 + Psyr_1030 0.40 -0.3 1,162,116 - Psyr_1030 0.40 +0.1 1,162,116 - Psyr_1030 0.40 -1.8 1,162,116 - Psyr_1030 0.40 +0.8 1,162,169 + Psyr_1030 0.42 -0.4 1,162,170 - Psyr_1030 0.43 -0.4 1,162,170 - Psyr_1030 0.43 +0.6 1,162,170 - Psyr_1030 0.43 -0.4 1,162,213 - Psyr_1030 0.45 +0.1 1,162,213 - Psyr_1030 0.45 -0.4
Or see this region's nucleotide sequence